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Rat Anti-IL6R Recombinant Antibody (15A7) (CBMAB-1042-LY)

This product is rat antibody recognizes mouse IL-6R. The antibody 15A7 immunoassay techniques such as: Blocking.
See all IL6R antibodies

Summary

Host Animal
Rat
Specificity
Mouse
Clone
15A7
Antibody Isotype
IgG2b
Application
Blocking

Basic Information

Specificity
Mouse
Antibody Isotype
IgG2b
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Purity
> 95% Purity determined by SDS-PAGE.
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name
Interleukin 6 Receptor
Introduction
Part of the receptor for interleukin 6. Binds to IL6 with low affinity, but does not transduce a signal. Signal activation necessitate an association with IL6ST. Activation may lead to the regulation of the immune response, acute-phase reactions and hematopoiesis.
Entrez Gene ID
16194
UniProt ID
P22272
Alternative Names
Il6r; CD126; IL-6R; IL-6RA; IL-6R-alpha
Function
Part of the receptor for interleukin 6. Binds to IL6 with low affinity, but does not transduce a signal (PubMed:28265003).
Signal activation necessitate an association with IL6ST. Activation leads to the regulation of the immune response, acute-phase reactions and hematopoiesis (PubMed:30995492, PubMed:31235509).
The interaction with membrane-bound IL6R and IL6ST stimulates 'classic signaling', the restricted expression of the IL6R limits classic IL6 signaling to only a few tissues such as the liver and some cells of the immune system. Whereas the binding of IL6 and soluble IL6R to IL6ST stimulates 'trans-signaling'. Alternatively, 'cluster signaling' occurs when membrane-bound IL6:IL6R complexes on transmitter cells activate IL6ST receptors on neighboring receiver cells (Probable).
Isoform 1
Signaling via the membrane-bound IL6R is mostly regenerative and anti-inflammatory (Probable). Drives naive CD4(+) T cells to the Th17 lineage, through 'cluster signaling' by dendritic cells (By similarity).
Isoform 2
Soluble form of IL6 receptor (sIL6R) that acts as an agonist of IL6 activity (PubMed:21990364).
The IL6:sIL6R complex (hyper-IL6) binds to IL6ST/gp130 on cell surfaces and induces signaling also on cells that do not express membrane-bound IL6R in a process called IL6 'trans-signaling'. sIL6R is causative for the proinflammatory properties of IL6 and an important player in the development of chronic inflammatory diseases (PubMed:21990364).
In complex with IL6, is required for induction of VEGF production (PubMed:12794819).
Plays a protective role during liver injury, being required for maintenance of tissue regeneration (By similarity).
'Trans-signaling' in central nervous system regulates energy and glucose homeostasis (By similarity).
Soluble interleukin-6 receptor subunit alpha
Soluble form of IL6 receptor (sIL6R) that acts as an agonist of IL6 activity (PubMed:21990364).
The IL6:sIL6R complex (hyper-IL6) binds to IL6ST/gp130 on cell surfaces and induces signaling also on cells that do not express membrane-bound IL6R in a process called IL6 'trans-signaling'. sIL6R is causative for the proinflammatory properties of IL6 and an important player in the development of chronic inflammatory diseases (PubMed:21990364).
In complex with IL6, is required for induction of VEGF production (PubMed:12794819).
Plays a protective role during liver injury, being required for maintenance of tissue regeneration (By similarity).
'Trans-signaling' in central nervous system regulates energy and glucose homeostasis (By similarity).
Biological Process
Acute-phase responseManual Assertion Based On ExperimentTAS:BHF-UCL
Ciliary neurotrophic factor-mediated signaling pathwayManual Assertion Based On ExperimentIMP:BHF-UCL
Cytokine-mediated signaling pathwayManual Assertion Based On ExperimentIDA:BHF-UCL
Defense response to Gram-negative bacteriumManual Assertion Based On ExperimentTAS:BHF-UCL
Defense response to Gram-positive bacteriumNAS:BHF-UCL
Endocrine pancreas developmentManual Assertion Based On ExperimentIMP:BHF-UCL
Extrinsic apoptotic signaling pathwayManual Assertion Based On ExperimentTAS:BHF-UCL
Hepatic immune responseManual Assertion Based On ExperimentTAS:BHF-UCL
Interleukin-6-mediated signaling pathwayManual Assertion Based On ExperimentIDA:ARUK-UCL
Monocyte chemotaxis1 PublicationIC:BHF-UCL
Negative regulation of collagen biosynthetic processIDA:BHF-UCL
Negative regulation of interleukin-8 productionNAS:BHF-UCL
Neutrophil mediated immunityTAS:BHF-UCL
Positive regulation of activation of Janus kinase activityManual Assertion Based On ExperimentIDA:BHF-UCL
Positive regulation of cell population proliferationManual Assertion Based On ExperimentIDA:BHF-UCL
Positive regulation of chemokine productionManual Assertion Based On ExperimentIDA:BHF-UCL
Positive regulation of glomerular mesangial cell proliferationManual Assertion Based On ExperimentIMP:ARUK-UCL
Positive regulation of interleukin-6 productionManual Assertion Based On ExperimentIDA:BHF-UCL
Positive regulation of leukocyte chemotaxisManual Assertion Based On ExperimentTAS:BHF-UCL
Positive regulation of MAPK cascadeIDA:BHF-UCL
Positive regulation of osteoblast differentiationManual Assertion Based On ExperimentTAS:BHF-UCL
Positive regulation of peptidyl-tyrosine phosphorylationManual Assertion Based On ExperimentIDA:BHF-UCL
Positive regulation of platelet aggregationIDA:ARUK-UCL
Positive regulation of smooth muscle cell proliferationManual Assertion Based On ExperimentIDA:BHF-UCL
Positive regulation of tyrosine phosphorylation of STAT proteinManual Assertion Based On ExperimentIMP:BHF-UCL
Response to cytokineManual Assertion Based On ExperimentIDA:BHF-UCL
T-helper 17 cell lineage commitmentIEA:Ensembl
Vascular endothelial growth factor productionManual Assertion Based On ExperimentIDA:UniProtKB
Cellular Location
Isoform 1: Cell membrane
Isoform 2: Secreted
Soluble interleukin-6 receptor subunit alpha: Secreted
Involvement in disease
Hyper-IgE recurrent infection syndrome 5, autosomal recessive (HIES5):
An immunologic disorder characterized by recurrent sinopulmonary and deep skin infections, mostly caused by bacteria, including H. influenza and Staphylococcus aureus. Additional features include asthma, atopic dermatitis, and impaired inflammatory responses during infection. Disease onset is in early infancy.
Topology
Extracellular: 20-365
Helical: 366-386
Cytoplasmic: 387-468
PTM
A short soluble form is released from the membrane by proteolysis (PubMed:26876177).
The sIL6R is formed mostly by limited proteolysis of membrane-bound receptors, a process referred to as ectodomain shedding, but is also directly secreted from the cells after alternative mRNA splicing (PubMed:26876177, PubMed:28060820).
mIL6R is cleaved by the proteases ADAM10 and ADAM17 (PubMed:26876177, PubMed:28060820).
Glycosylated. Glycosylation is dispensable for transport, signaling, and cell-surface turnover. Glycosylation at Asn-55 is a protease-regulatory exosite. Glycosylation is required for ADAM17-mediated proteolysis.

Hamilton, F. W., Thomas, M., Arnold, D., Palmer, T., Moran, E., Mentzer, A. J., ... & Timpson, N. J. (2023). Therapeutic potential of IL6R blockade for the treatment of sepsis and sepsis-related death: A Mendelian randomisation study. PLoS Medicine, 20(1), e1004174.

Maiti, S., Banerjee, A., Nazmeen, A., Kanwar, M., & Das, S. (2022). Active-site molecular docking of nigellidine with nucleocapsid–NSP2–MPro of COVID-19 and to human IL1R–IL6R and strong antioxidant role of Nigella sativa in experimental rats. Journal of Drug Targeting, 30(5), 511-521.

Teoh, P. J., Chung, T. H., Chng, P. Y., Toh, S. H., & Chng, W. J. (2020). IL6R-STAT3-ADAR1 (P150) interplay promotes oncogenicity in multiple myeloma with 1q21 amplification. haematologica, 105(5), 1391.

Strafella, C., Caputo, V., Termine, A., Barati, S., Caltagirone, C., Giardina, E., & Cascella, R. (2020). Investigation of genetic variations of IL6 and IL6R as potential prognostic and pharmacogenetics biomarkers: implications for COVID-19 and neuroinflammatory disorders. Life, 10(12), 351.

Nouri, B., Nair, N., & Barton, A. (2020). Predicting treatment response to IL6R blockers in rheumatoid arthritis. Rheumatology, 59(12), 3603-3610.

Buonaguro, F. M., Puzanov, I., & Ascierto, P. A. (2020). Anti-IL6R role in treatment of COVID-19-related ARDS. Journal of translational medicine, 18, 1-2.

Jia, C., Wang, G., Wang, T., Fu, B., Zhang, Y., Huang, L., ... & Liu, W. (2020). Cancer-associated Fibroblasts induce epithelial-mesenchymal transition via the Transglutaminase 2-dependent IL-6/IL6R/STAT3 axis in Hepatocellular Carcinoma. International journal of biological sciences, 16(14), 2542.

Deng, N., Li, L., Gao, J., Zhou, J., Wang, Y., Wang, C., & Liu, Y. (2018). Hsa_circ_0009910 promotes carcinogenesis by promoting the expression of miR-449a target IL6R in osteosarcoma. Biochemical and biophysical research communications, 495(1), 189-196.

Tamura, Y., Phan, C., Tu, L., Le Hiress, M., Thuillet, R., Jutant, E. M., ... & Guignabert, C. (2018). Ectopic upregulation of membrane-bound IL6R drives vascular remodeling in pulmonary arterial hypertension. The Journal of clinical investigation, 128(5), 1956-1970.

Zhang, B., Yu, L., Han, N., Hu, Z., Wang, S., Ding, L., & Jiang, J. (2018). LINC01116 targets miR-520a-3p and affects IL6R to promote the proliferation and migration of osteosarcoma cells through the Jak-stat signaling pathway. Biomedicine & Pharmacotherapy, 107, 270-282.

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For research use only. Not intended for any clinical use.

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