Mouse Anti-INPP5A Recombinant Antibody (3D8) (CBMAB-A4488-LY)

The product is antibody recognizes INPP5A. The antibody 3D8 immunoassay techniques such as: WB, ELISA.
See all INPP5A antibodies

Datasheet

Summary

Host Animal

Mouse

Specificity

Human

Clone

3D8

Antibody Isotype

IgG2a, κ

Application

WB, ELISA

Basic Information

Immunogen

INPP5A (NP_005530, 288 a.a. ~ 387 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.

Specificity

Human

Antibody Isotype

IgG2a, κ

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Purity

> 95% Purity determined by SDS-PAGE.

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name

inositol polyphosphate-5-phosphatase, 40kDa

Introduction

The protein encoded by this gene is a membrane-associated type I inositol 1,4,5-trisphosphate (InsP3) 5-phosphatase. InsP3 5-phosphatases hydrolyze Ins(1,4,5)P3, which mobilizes intracellular calcium and acts as a second messenger mediating cell responses to various stimulation. [provided by RefSeq]

Entrez Gene ID

3632

UniProt ID

Q14642

Alternative Names

5PTASE; DKFZp434A1721; MGC116947; MGC116949

Function

Phosphatase that specifically hydrolyzes the 5-phosphate of inositol 1,4,5-trisphosphate to inositol 1,4-bisphosphate, and inositol 1,3,4,5-tetrasphosphate to inositol 1,3,4-trisphosphate (PubMed:8013665, PubMed:8769125, PubMed:8626616).
Plays a crucial role in the survival of cerebellar Purkinje cells (By similarity).

Biological Process

Inositol phosphate dephosphorylationManual Assertion Based On ExperimentIDA:MGI
Inositol phosphate metabolic processTAS:Reactome
Inositol phosphate-mediated signalingManual Assertion Based On ExperimentIBA:GO_Central
Negative regulation of neuron deathISS:UniProtKB
Phosphatidylinositol dephosphorylationIEA:InterPro

PTM

Isoprenylation at Cys-409 is required for localization at the membrane.

References

Ardalan Khales, S., Aarabi, A., Abbaszadegan, M. R., & Forghanifard, M. M. (2022). INPP5A/HLA-G1/IL-10/MMP-21 Axis in Progression of Esophageal Squamous Cell Carcinoma. Iranian Biomedical Journal, 26(6), 440-453.

Golyan, F. F., & Forghanifard, M. M. (2021). A new gene panel as a marker for ESCC poor prognosis; INPP5A, TWIST1, MMP2, and EGFR. Advances in Medical Sciences, 66(2), 231-236.

Fernandez, A., Drozd, M., Thümmler, S., Bardoni, B., Askenazy, F., & Capovilla, M. (2021). A novel microduplication in INPP5A segregates with schizophrenia spectrum disorder in the family of a patient with both childhood onset schizophrenia and autism spectrum disorder. American Journal of Medical Genetics Part A, 185(6), 1841-1847.

Liu, Q., Huang, S., Yin, P., Yang, S., Zhang, J., Jing, L., ... & Li, S. (2020). Cerebellum-enriched protein INPP5A contributes to selective neuropathology in mouse model of spinocerebellar ataxias type 17. Nature communications, 11(1), 1101.

Liang, H. J., DiCaudo, D. J., Schmidt, J. E., Pittelkow, M. R., Sekulic, A., & Mangold, A. R. (2018). INPP5a expression as a prognostic marker in cutaneous squamous cell carcinoma (cSCC).

Yang, M., Zhai, X., Ge, T., Yang, C., & Lou, G. (2018). miR-181a-5p promotes proliferation and invasion and inhibits apoptosis of cervical cancer cells via regulating inositol polyphosphate-5-phosphatase A (INPP5A). Oncology Research Featuring Preclinical and Clinical Cancer Therapeutics, 26(5), 703-712.

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Protocols

Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Associated Products

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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