Rabbit Anti-IREB2 Recombinant Antibody (CBYY-I1680) (CBMAB-I2850-YY)

Name: Rabbit Anti-IREB2 Recombinant Antibody (CBYY-I1680)
SKU: CBMAB-I2850-YY
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Rabbit

Clone

CBYY-I1680

Application

Specificity

Human

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

10 mM sodium HEPES, pH 7.5, 100 ug/ml BSA, 150 mM NaCl, 50% glycerol

Preservative

0.02% sodium azide

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

More Infomation

Target

Full Name

Iron Responsive Element Binding Protein 2

Introduction

The protein encoded by this gene is an RNA-binding protein that acts to regulate iron levels in the cells by regulating the translation and stability of mRNAs that affect iron homeostasis under conditions when iron is depleted. When iron levels are low, this protein binds to iron-responsive elements (IRES), stem-loop structures located either in the 5' or 3' UTRs. Binding to the 5' UTR represses translation, while binding to the 3' UTR inhibits mRNA degradation. When iron is found in the cell, this protein is degraded in a F-box and leucine rich repeat protein 5-dependent manner. Variants in this gene have been associated with lung cancer and chronic obstructive pulmonary disease (COPD). Alternative splicing results in multiple transcript variants encoding different isoforms.

Entrez Gene ID

3658

UniProt ID

P48200

Alternative Names

Iron Responsive Element Binding Protein 2

Function

RNA-binding protein that binds to iron-responsive elements (IRES), which are stem-loop structures found in the 5'-UTR of ferritin, and delta aminolevulinic acid synthase mRNAs, and in the 3'-UTR of transferrin receptor mRNA. Binding to the IRE element in ferritin results in the repression of its mRNA translation. Binding of the protein to the transferrin receptor mRNA inhibits the degradation of this otherwise rapidly degraded mRNA.

Biological Process

Cellular iron ion homeostasisIEA:Ensembl
Citrate metabolic processManual Assertion Based On ExperimentIBA:GO_Central
Erythrocyte homeostasisIEA:Ensembl
Intestinal absorptionIEA:Ensembl
Iron ion homeostasisManual Assertion Based On ExperimentIMP:UniProtKB
Iron ion transportManual Assertion Based On ExperimentTAS:UniProtKB
Osteoclast differentiationIEA:Ensembl
Post-embryonic developmentIEA:Ensembl
Protoporphyrinogen IX biosynthetic processIEA:Ensembl
Tricarboxylic acid cycleManual Assertion Based On ExperimentIBA:GO_Central

Cellular Location

Cytoplasm

Involvement in disease

Neurodegeneration, early-onset, with choreoathetoid movements and microcytic anemia (NDCAMA):
An autosomal recessive disorder characterized by severe neurological and extra-neurological manifestations. Clinical features include early-onset global developmental delay, absent speech, dystonia, spasticity, choreoathetoid movement disorder, seizures, and microcytic hypochromic anaemia unresponsive to iron supplementation.

PTM

Ubiquitinated and degraded by the proteasome in presence of high level of iron and oxygen. Ubiquitinated by a SCF complex containing FBXL5. Upon iron and oxygen depletion FBXL5 is degraded, preventing ubiquitination and allowing its RNA-binding activity.

Xia, H., Wu, Y., Zhao, J., Cheng, C., Lin, J., Yang, Y., ... & Liu, Q. (2023). N6-methyladenosine-modified circSAV1 triggers ferroptosis in COPD through recruiting YTHDF1 to facilitate the translation of IREB2. Cell Death & Differentiation, 30(5), 1293-1304.

Zhu, T., Xiao, Z., Yuan, H., Tian, H., Chen, T., Chen, Q., ... & Zhou, J. (2022). ACO1 and IREB2 downregulation confer poor prognosis and correlate with autophagy-related ferroptosis and immune infiltration in KIRC. Frontiers in oncology, 12, 929838.

Fan, H., Ai, R., Mu, S., Niu, X., Guo, Z., & Liu, L. (2022). MiR-19a suppresses ferroptosis of colorectal cancer cells by targeting IREB2. Bioengineered, 13(5), 12021-12029.

Mishima, E. (2022). The E2F1-IREB2 axis regulates neuronal ferroptosis in cerebral ischemia. Hypertension Research, 45(6), 1085-1086.

Maio, N., Saneto, R. P., Steet, R., Sotero de Menezes, M. A., Skinner, C., & Rouault, T. A. (2022). Disruption of cellular iron homeostasis by IREB2 missense variants causes severe neurodevelopmental delay, dystonia and seizures. Brain Communications, 4(3), fcac102.

Zeng, Q., Chen, Q., Zou, D., Guo, R., Xiao, D., Jiang, S., ... & Ma, G. (2020). Different associations between the IREB2 variants and chronic obstructive pulmonary disease susceptibility. Frontiers in Genetics, 11, 598053.

Cooper, M. S., Stark, Z., Lunke, S., Zhao, T., & Amor, D. J. (2019). IREB2-associated neurodegeneration. Brain, 142(8), e40-e40.

Liu, F., Chen, Y., Chen, B., Liu, C., & Xing, J. (2019). RETRACTED ARTICLE: MiR-935 Promotes Clear Cell Renal Cell Carcinoma Migration and Invasion by Targeting IREB2. Cancer management and research, 10891-10900.

Maio, N., Ghosh, M. C., Costain, G., Carnevale, A., Si, Y., Yoon, G., & Rouault, T. A. (2019). Reply: IREB2-associated neurodegeneration. Brain, 142(8), e41-e41.

Zhang, Y., Qiu, J., Zhang, P., Zhang, J., Jiang, M., & Ma, Z. (2018). Genetic variants in FAM13A and IREB2 are associated with the susceptibility to COPD in a Chinese rural population: a case-control study. International journal of chronic obstructive pulmonary disease, 1735-1745.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
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Isotype control

For research use only. Not intended for any clinical use.

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