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Rabbit Anti-IREB2 Recombinant Antibody (D6E6W) (CBMAB-CP1203-LY)

The product is antibody recognizes IREB2. The antibody D6E6W immunoassay techniques such as: WB.
See all IREB2 antibodies

Summary

Host Animal
Rabbit
Specificity
Human
Clone
D6E6W
Antibody Isotype
IgG
Application
WB

Basic Information

Immunogen
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro731 of human IRP2 protein.
Specificity
Human
Antibody Isotype
IgG
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
100 µg/ml BSA, 50% glycerol
Preservative
0.02% sodium azide
Purity
> 95% Purity determined by SDS-PAGE.
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name
Iron Responsive Element Binding Protein 2
Introduction
The protein encoded by this gene is an RNA-binding protein that acts to regulate iron levels in the cells by regulating the translation and stability of mRNAs that affect iron homeostasis under conditions when iron is depleted. When iron levels are low, this protein binds to iron-responsive elements (IRES), stem-loop structures located either in the 5' or 3' UTRs. Binding to the 5' UTR represses translation, while binding to the 3' UTR inhibits mRNA degradation. When iron is found in the cell, this protein is degraded in a F-box and leucine rich repeat protein 5-dependent manner. Variants in this gene have been associated with lung cancer and chronic obstructive pulmonary disease (COPD). Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Aug 2017]
Entrez Gene ID
3658
UniProt ID
P48200
Alternative Names
Iron Responsive Element Binding Protein 2
Function
RNA-binding protein that binds to iron-responsive elements (IRES), which are stem-loop structures found in the 5'-UTR of ferritin, and delta aminolevulinic acid synthase mRNAs, and in the 3'-UTR of transferrin receptor mRNA. Binding to the IRE element in ferritin results in the repression of its mRNA translation. Binding of the protein to the transferrin receptor mRNA inhibits the degradation of this otherwise rapidly degraded mRNA.
Biological Process
Cellular iron ion homeostasisIEA:Ensembl
Citrate metabolic processManual Assertion Based On ExperimentIBA:GO_Central
Erythrocyte homeostasisIEA:Ensembl
Intestinal absorptionIEA:Ensembl
Iron ion homeostasisManual Assertion Based On ExperimentIMP:UniProtKB
Iron ion transportManual Assertion Based On ExperimentTAS:UniProtKB
Osteoclast differentiationIEA:Ensembl
Post-embryonic developmentIEA:Ensembl
Protoporphyrinogen IX biosynthetic processIEA:Ensembl
Tricarboxylic acid cycleManual Assertion Based On ExperimentIBA:GO_Central
Cellular Location
Cytoplasm
Involvement in disease
Neurodegeneration, early-onset, with choreoathetoid movements and microcytic anemia (NDCAMA):
An autosomal recessive disorder characterized by severe neurological and extra-neurological manifestations. Clinical features include early-onset global developmental delay, absent speech, dystonia, spasticity, choreoathetoid movement disorder, seizures, and microcytic hypochromic anaemia unresponsive to iron supplementation.
PTM
Ubiquitinated and degraded by the proteasome in presence of high level of iron and oxygen. Ubiquitinated by a SCF complex containing FBXL5. Upon iron and oxygen depletion FBXL5 is degraded, preventing ubiquitination and allowing its RNA-binding activity.

Xia, H., Wu, Y., Zhao, J., Cheng, C., Lin, J., Yang, Y., ... & Liu, Q. (2023). N6-methyladenosine-modified circSAV1 triggers ferroptosis in COPD through recruiting YTHDF1 to facilitate the translation of IREB2. Cell Death & Differentiation, 30(5), 1293-1304.

Zhu, T., Xiao, Z., Yuan, H., Tian, H., Chen, T., Chen, Q., ... & Zhou, J. (2022). ACO1 and IREB2 downregulation confer poor prognosis and correlate with autophagy-related ferroptosis and immune infiltration in KIRC. Frontiers in oncology, 12, 929838.

Fan, H., Ai, R., Mu, S., Niu, X., Guo, Z., & Liu, L. (2022). MiR-19a suppresses ferroptosis of colorectal cancer cells by targeting IREB2. Bioengineered, 13(5), 12021-12029.

Mishima, E. (2022). The E2F1-IREB2 axis regulates neuronal ferroptosis in cerebral ischemia. Hypertension Research, 45(6), 1085-1086.

Maio, N., Saneto, R. P., Steet, R., Sotero de Menezes, M. A., Skinner, C., & Rouault, T. A. (2022). Disruption of cellular iron homeostasis by IREB2 missense variants causes severe neurodevelopmental delay, dystonia and seizures. Brain Communications, 4(3), fcac102.

Zeng, Q., Chen, Q., Zou, D., Guo, R., Xiao, D., Jiang, S., ... & Ma, G. (2020). Different associations between the IREB2 variants and chronic obstructive pulmonary disease susceptibility. Frontiers in Genetics, 11, 598053.

Cooper, M. S., Stark, Z., Lunke, S., Zhao, T., & Amor, D. J. (2019). IREB2-associated neurodegeneration. Brain, 142(8), e40-e40.

Liu, F., Chen, Y., Chen, B., Liu, C., & Xing, J. (2019). RETRACTED ARTICLE: MiR-935 Promotes Clear Cell Renal Cell Carcinoma Migration and Invasion by Targeting IREB2. Cancer management and research, 10891-10900.

Maio, N., Ghosh, M. C., Costain, G., Carnevale, A., Si, Y., Yoon, G., & Rouault, T. A. (2019). Reply: IREB2-associated neurodegeneration. Brain, 142(8), e41-e41.

Zhang, Y., Qiu, J., Zhang, P., Zhang, J., Jiang, M., & Ma, Z. (2018). Genetic variants in FAM13A and IREB2 are associated with the susceptibility to COPD in a Chinese rural population: a case-control study. International journal of chronic obstructive pulmonary disease, 1735-1745.

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For research use only. Not intended for any clinical use.

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