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Mouse Anti-IRF5 Recombinant Antibody (CBYY-I1902) (CBMAB-I3072-YY)

This product is Mouse antibody that recognizes IRF5. The antibody CBYY-I1902 can be used for immunoassay techniques such as: WB
See all IRF5 antibodies

Summary

Host Animal
Mouse
Specificity
Human
Clone
CBYY-I1902
Antibody Isotype
IgG1
Application
WB

Basic Information

Immunogen
Full length human recombinant protein of human IRF5(NP_116032)
Specificity
Human
Antibody Isotype
IgG1
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
PBS, PH 7.3, 1% BSA, 50% glycerol
Preservative
0.02% sodium azide
Concentration
1 mg/ml
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
Interferon Regulatory Factor 5
Introduction
This gene encodes a member of the interferon regulatory factor (IRF) family, a group of transcription factors with diverse roles, including virus-mediated activation of interferon, and modulation of cell growth, differentiation, apoptosis, and immune system activity. Members of the IRF family are characterized by a conserved N-terminal DNA-binding domain containing tryptophan (W) repeats. Alternative promoter use and alternative splicing result in multiple transcript variants, and a 30-nt indel polymorphism (SNP rs60344245) can result in loss of a 10-aa segment.
Entrez Gene ID
3663
UniProt ID
Q13568
Alternative Names
Interferon Regulatory Factor 5
Function
Transcription factor that plays a critical role in innate immunity by activating expression of type I interferon (IFN) IFNA and INFB and inflammatory cytokines downstream of endolysosomal toll-like receptors TLR7, TLR8 and TLR9 (PubMed:11303025, PubMed:15695821, PubMed:22412986, PubMed:25326418, PubMed:32433612).
Regulates the transcription of type I IFN genes (IFN-alpha and IFN-beta) and IFN-stimulated genes (ISG) by binding to an interferon-stimulated response element (ISRE) in their promoters (By similarity).
Can efficiently activate both the IFN-beta (IFNB) and the IFN-alpha (IFNA) genes and mediate their induction downstream of the TLR-activated, MyD88-dependent pathway (By similarity).
Key transcription factor regulating the IFN response during SARS-CoV-2 infection (PubMed:33440148).
Biological Process
Cytokine-mediated signaling pathwayManual Assertion Based On ExperimentIDA:UniProtKB
Defense response to virusIEA:UniProtKB-KW
Immune system processManual Assertion Based On ExperimentIBA:GO_Central
Inflammatory responseIEA:UniProtKB-KW
Innate immune responseISS:UniProtKB
Positive regulation of apoptotic processManual Assertion Based On ExperimentIMP:BHF-UCL
Positive regulation of interferon-alpha production1 PublicationIC:BHF-UCL
Positive regulation of interferon-beta production1 PublicationIC:BHF-UCL
Positive regulation of interleukin-12 production1 PublicationIC:BHF-UCL
Positive regulation of transcription by RNA polymerase IIManual Assertion Based On ExperimentIDA:UniProtKB
Positive regulation of type I interferon productionManual Assertion Based On ExperimentIDA:UniProtKB
Regulation of transcription by RNA polymerase IIManual Assertion Based On ExperimentIBA:GO_Central
Response to muramyl dipeptideManual Assertion Based On ExperimentIDA:BHF-UCL
Response to peptidoglycanManual Assertion Based On ExperimentIDA:BHF-UCL
Cellular Location
Cytoplasm; Nucleus. Shuttles between the nucleus and the cytoplasm: upon activation by the TLR adapter MYD88 and subsequent phosphorylation, translocates to the nucleus.
Involvement in disease
Inflammatory bowel disease 14 (IBD14):
A chronic, relapsing inflammation of the gastrointestinal tract with a complex etiology. It is subdivided into Crohn disease and ulcerative colitis phenotypes. Crohn disease may affect any part of the gastrointestinal tract from the mouth to the anus, but most frequently it involves the terminal ileum and colon. Bowel inflammation is transmural and discontinuous; it may contain granulomas or be associated with intestinal or perianal fistulas. In contrast, in ulcerative colitis, the inflammation is continuous and limited to rectal and colonic mucosal layers; fistulas and granulomas are not observed. Both diseases include extraintestinal inflammation of the skin, eyes, or joints.
Systemic lupus erythematosus 10 (SLEB10):
A chronic, relapsing, inflammatory, and often febrile multisystemic disorder of connective tissue, characterized principally by involvement of the skin, joints, kidneys and serosal membranes. It is of unknown etiology, but is thought to represent a failure of the regulatory mechanisms of the autoimmune system. The disease is marked by a wide range of system dysfunctions, an elevated erythrocyte sedimentation rate, and the formation of LE cells in the blood or bone marrow.
Rheumatoid arthritis (RA):
An inflammatory disease with autoimmune features and a complex genetic component. It primarily affects the joints and is characterized by inflammatory changes in the synovial membranes and articular structures, widespread fibrinoid degeneration of the collagen fibers in mesenchymal tissues, and by atrophy and rarefaction of bony structures.
PTM
Phosphorylation of serine and threonine residues by IKBKB in a C-terminal autoinhibitory region, stimulates dimerization, transport into the nucleus, assembly with the coactivator CBP/EP300 and initiation of transcription.
'Lys-63'-linked polyubiquitination by TRAF6 is required for activation.

Edsfeldt, A., Swart, M., Singh, P., Dib, L., Sun, J., Cole, J. E., ... & Monaco, C. (2022). Interferon regulatory factor-5-dependent CD11c+ macrophages contribute to the formation of rupture–prone atherosclerotic plaques. European heart journal, 43(19), 1864-1877.

Posadas-Sánchez, R., Cardoso-Saldaña, G., Fragoso, J. M., & Vargas-Alarcón, G. (2021). Interferon regulatory factor 5 (IRF5) gene haplotypes are associated with premature coronary artery disease. Association of the IRF5 polymorphisms with cardiometabolic parameters. The genetics of atherosclerotic disease (GEA) Mexican study. Biomolecules, 11(3), 443.

Wang, Q., Fang, P., He, R., Li, M., Yu, H., Zhou, L., ... & Liu, S. (2020). O-GlcNAc transferase promotes influenza A virus–induced cytokine storm by targeting interferon regulatory factor–5. Science Advances, 6(16), eaaz7086.

Fan, Z., Zhao, S., Zhu, Y., Li, Z., Liu, Z., Yan, Y., ... & Zhang, B. (2020). Interferon regulatory factor 5 mediates lipopolysaccharide-induced neuroinflammation. Frontiers in Immunology, 11, 600479.

Sindhu, S., Thomas, R., Kochumon, S., Wilson, A., Abu-Farha, M., Bennakhi, A., ... & Ahmad, R. (2019). Increased adipose tissue expression of interferon regulatory factor (IRF)-5 in obesity: association with metabolic inflammation. Cells, 8(11), 1418.

Guo, L., Hao, R., Tian, F., An, N., & Wang, K. (2018). Interferon regulatory factor 5 (IRF5) regulates the expression of matrix metalloproteinase-3 (MMP-3) in human chondrocytes. International Immunopharmacology, 55, 231-236.

Ai, K., Luo, K., Xia, L., Gao, W., Hu, W., Qi, Z., & Xu, Q. (2018). Functional characterization of interferon regulatory factor 5 and its role in the innate antiviral immune response. Fish & Shellfish Immunology, 72, 31-36.

Wang, X., Guo, J., Wang, Y., Xiao, Y., Wang, L., & Hua, S. (2018). Expression levels of interferon regulatory factor 5 (IRF5) and related inflammatory cytokines associated with severity, prognosis, and causative pathogen in patients with community-acquired pneumonia. Medical science monitor: international medical journal of experimental and clinical research, 24, 3620.

Ban, T., Sato, G. R., & Tamura, T. (2018). Regulation and role of the transcription factor IRF5 in innate immune responses and systemic lupus erythematosus. International Immunology, 30(11), 529-536.

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For research use only. Not intended for any clinical use.

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