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Mouse Anti-IRF8 Recombinant Antibody (CBYY-I0297) (CBMAB-I0825-YY)

This product is Mouse antibody that recognizes IRF8. The antibody CBYY-I0297 can be used for immunoassay techniques such as: WB, FC, IF
See all IRF8 antibodies

Summary

Host Animal
Mouse
Specificity
Human
Clone
CBYY-I0297
Antibody Isotype
IgG1
Application
WB, FC, IF

Basic Information

Specificity
Human
Antibody Isotype
IgG1
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Concentration
1 mg/ml
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
IRF8
Introduction
Interferon consensus sequence-binding protein (ICSBP) is a transcription factor of the interferon (IFN) regulatory factor (IRF) family. Proteins of this family are composed of a conserved DNA-binding domain in the N-terminal region and a divergent C-terminal region that serves as the regulatory domain. The IRF family proteins bind to the IFN-stimulated response element (ISRE) and regulate expression of genes stimulated by type I IFNs, namely IFN-alpha and IFN-beta. IRF family proteins also control expression of IFN-alpha and IFN-beta-regulated genes that are induced by viral infection.
Entrez Gene ID
3394
UniProt ID
Q02556
Alternative Names
Interferon Regulatory Factor 8
Function
Transcription factor that specifically binds to the upstream regulatory region of type I interferon (IFN) and IFN-inducible MHC class I genes (the interferon consensus sequence (ICS)) (PubMed:25122610).
Can both act as a transcriptional activator or repressor (By similarity).
Plays a negative regulatory role in cells of the immune system (By similarity).
Involved in CD8(+) dendritic cell differentiation by forming a complex with the BATF-JUNB heterodimer in immune cells, leading to recognition of AICE sequence (5'-TGAnTCA/GAAA-3'), an immune-specific regulatory element, followed by cooperative binding of BATF and IRF8 and activation of genes (By similarity).
Required for the development of plasmacytoid dendritic cells (pDCs), which produce most of the type I IFN in response to viral infection (By similarity).
Positively regulates macroautophagy in dendritic cells (PubMed:29434592).
Biological Process
AutophagyIEA:UniProtKB-KW
Cellular response to interferon-gammaManual Assertion Based On ExperimentIDA:UniProtKB
Cellular response to lipopolysaccharideIEA:Ensembl
Defense response to bacteriumIEA:Ensembl
Defense response to protozoanIEA:Ensembl
Dendritic cell differentiationISS:UniProtKB
Follicular B cell differentiationIEA:Ensembl
Germinal center B cell differentiationIEA:Ensembl
Immune responseManual Assertion Based On ExperimentTAS:ProtInc
Immune system processManual Assertion Based On ExperimentIBA:GO_Central
Myeloid cell differentiationIEA:Ensembl
Negative regulation of transcription by RNA polymerase IIManual Assertion Based On ExperimentIDA:UniProtKB
PhagocytosisIEA:Ensembl
Plasmacytoid dendritic cell differentiationISS:UniProtKB
Positive regulation of interferon-gamma productionIEA:Ensembl
Positive regulation of interleukin-12 productionIEA:Ensembl
Positive regulation of transcription by RNA polymerase IIManual Assertion Based On ExperimentIDA:UniProtKB
Regulation of transcription by RNA polymerase IIManual Assertion Based On ExperimentIBA:GO_Central
Regulation of type I interferon productionISS:UniProtKB
Cellular Location
Cytoplasm; Nucleus. In resting macrophages, localizes in the cytoplasm. Translocated in the nucleus upon IFN-gamma induction.
Involvement in disease
Immunodeficiency 32A (IMD32A):
An immunologic disorder characterized by abnormal peripheral blood myeloid phenotype with a marked loss of CD11C-positive/CD1C dendritic cells, resulting in selective susceptibility to mycobacterial infections.
Immunodeficiency 32B (IMD32B):
An autosomal recessive primary immunodeficiency characterized by monocyte and dendritic cell deficiency, myeloproliferation, and susceptibility to severe opportunistic infections, including disseminated BCG infection and oral candidiasis.
PTM
Ubiquitinated (PubMed:25122610).
Ubiquitination by TRIM21 in macrophages, a process that is strongly increased upon interferon gamma stimulation, leds to the enhanced transcriptional activity of target cytokine genes (By similarity).
Ubiquitination leads to its degradation by the proteasome (PubMed:25122610).
Sumoylated with SUMO3. Desumoylated by SENP1.

Friebus-Kardash, J., Kuang, F., Peitz, T., Hamdan, T. A., Eisenberger, U., Boss, K., ... & Jahn, M. (2023). Expression of Interferon Regulatory Factor 8 (IRF8) and Its Association with Infections in Dialysis Patients. Cells, 12(14), 1892.

Feinberg, P. A., Becker, S. C., Chung, L., Ferrari, L., Stellwagen, D., Anaclet, C., ... & Schafer, D. P. (2022). Elevated TNF-α leads to neural circuit instability in the absence of Interferon Regulatory Factor 8. Journal of Neuroscience, 42(32), 6171-6185.

Wu, H., Li, Y., Shi, G., Du, S., Wang, X., Ye, W., ... & Yang, Y. (2022). Hepatic interferon regulatory factor 8 expression suppresses hepatocellular carcinoma progression and enhances the response to anti–programmed cell death protein‐1 therapy. Hepatology, 76(6), 1602-1616.

Tripathi, A., Singh Rawat, B., Addya, S., Surjit, M., Tailor, P., Vrati, S., & Banerjee, A. (2021). Lack of Interferon (IFN) regulatory factor 8 associated with restricted IFN-γ response augmented Japanese encephalitis virus replication in the mouse brain. Journal of Virology, 95(21), 10-1128.

Shi, G., Zhang, Z., Ma, S., Li, Y., Du, S., Chu, Y., ... & Wu, H. (2021). Hepatic interferon regulatory factor 8 expression mediates liver ischemia/reperfusion injury in mice. Biochemical Pharmacology, 192, 114728.

Xia, X., Wang, W., Yin, K., & Wang, S. (2020). Interferon regulatory factor 8 governs myeloid cell development. Cytokine & Growth Factor Reviews, 55, 48-57.

Twum, D. Y., Colligan, S. H., Hoffend, N. C., Katsuta, E., Gomez, E. C., Hensen, M. L., ... & Abrams, S. I. (2019). IFN regulatory factor–8 expression in macrophages governs an antimetastatic program. Jci Insight, 4(3).

Muhitch, J. B., Hoffend, N. C., Azabdaftari, G., Miller, A., Bshara, W., Morrison, C. D., ... & Abrams, S. I. (2019). Tumor-associated macrophage expression of interferon regulatory Factor-8 (IRF8) is a predictor of progression and patient survival in renal cell carcinoma. Journal for immunotherapy of cancer, 7(1), 1-9.

Clément, M., Haddad, Y., Raffort, J., Lareyre, F., Newland, S. A., Master, L., ... & Mallat, Z. (2018). Deletion of IRF8 (interferon regulatory factor 8)-dependent dendritic cells abrogates proatherogenic adaptive immunity. Circulation research, 122(6), 813-820.

Lv, D. W., Zhang, K., & Li, R. (2018). Interferon regulatory factor 8 regulates caspase-1 expression to facilitate Epstein-Barr virus reactivation in response to B cell receptor stimulation and chemical induction. PLoS pathogens, 14(1), e1006868.

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For research use only. Not intended for any clinical use.

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