Rabbit Anti-IRF8 Recombinant Antibody (CBYY-I0547) (CBMAB-I1231-YY)

Name: Rabbit Anti-IRF8 Recombinant Antibody (CBYY-I0547)
SKU: CBMAB-I1231-YY
Rating: 5 (1 reviews)

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Datasheet

SDS

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Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Rabbit

Clone

CBYY-I0547

Application

WB, IP, ChIP

Specificity

Human, Mouse, Rat, Monkey, Frog, Cattle

Antibody Isotype

IgG

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

More Infomation

Target

Full Name

IRF8

Introduction

Interferon consensus sequence-binding protein (ICSBP) is a transcription factor of the interferon (IFN) regulatory factor (IRF) family. Proteins of this family are composed of a conserved DNA-binding domain in the N-terminal region and a divergent C-terminal region that serves as the regulatory domain. The IRF family proteins bind to the IFN-stimulated response element (ISRE) and regulate expression of genes stimulated by type I IFNs, namely IFN-alpha and IFN-beta. IRF family proteins also control expression of IFN-alpha and IFN-beta-regulated genes that are induced by viral infection.

Entrez Gene ID

Human	3394
Mouse	15900
Rat	292060
Monkey	693933
Frog	100496217
Cattle	614909

UniProt ID

Human	Q02556
Mouse	P23611
Rat	Q5NUI4
Monkey	A0A0D9S209
Frog	Q6DE53
Cattle	A0A3S5ZP50

Alternative Names

Interferon Regulatory Factor 8

Function

Transcription factor that specifically binds to the upstream regulatory region of type I interferon (IFN) and IFN-inducible MHC class I genes (the interferon consensus sequence (ICS)) (PubMed:25122610).
Can both act as a transcriptional activator or repressor (By similarity).
Plays a negative regulatory role in cells of the immune system (By similarity).
Involved in CD8(+) dendritic cell differentiation by forming a complex with the BATF-JUNB heterodimer in immune cells, leading to recognition of AICE sequence (5'-TGAnTCA/GAAA-3'), an immune-specific regulatory element, followed by cooperative binding of BATF and IRF8 and activation of genes (By similarity).
Required for the development of plasmacytoid dendritic cells (pDCs), which produce most of the type I IFN in response to viral infection (By similarity).
Positively regulates macroautophagy in dendritic cells (PubMed:29434592).

Biological Process

AutophagyIEA:UniProtKB-KW
Cellular response to interferon-gammaManual Assertion Based On ExperimentIDA:UniProtKB
Cellular response to lipopolysaccharideIEA:Ensembl
Defense response to bacteriumIEA:Ensembl
Defense response to protozoanIEA:Ensembl
Dendritic cell differentiationISS:UniProtKB
Follicular B cell differentiationIEA:Ensembl
Germinal center B cell differentiationIEA:Ensembl
Immune responseManual Assertion Based On ExperimentTAS:ProtInc
Immune system processManual Assertion Based On ExperimentIBA:GO_Central
Myeloid cell differentiationIEA:Ensembl
Negative regulation of transcription by RNA polymerase IIManual Assertion Based On ExperimentIDA:UniProtKB
PhagocytosisIEA:Ensembl
Plasmacytoid dendritic cell differentiationISS:UniProtKB
Positive regulation of interferon-gamma productionIEA:Ensembl
Positive regulation of interleukin-12 productionIEA:Ensembl
Positive regulation of transcription by RNA polymerase IIManual Assertion Based On ExperimentIDA:UniProtKB
Regulation of transcription by RNA polymerase IIManual Assertion Based On ExperimentIBA:GO_Central
Regulation of type I interferon productionISS:UniProtKB

Cellular Location

Cytoplasm; Nucleus. In resting macrophages, localizes in the cytoplasm. Translocated in the nucleus upon IFN-gamma induction.

Involvement in disease

Immunodeficiency 32A (IMD32A):
An immunologic disorder characterized by abnormal peripheral blood myeloid phenotype with a marked loss of CD11C-positive/CD1C dendritic cells, resulting in selective susceptibility to mycobacterial infections.
Immunodeficiency 32B (IMD32B):
An autosomal recessive primary immunodeficiency characterized by monocyte and dendritic cell deficiency, myeloproliferation, and susceptibility to severe opportunistic infections, including disseminated BCG infection and oral candidiasis.

PTM

Ubiquitinated (PubMed:25122610).
Ubiquitination by TRIM21 in macrophages, a process that is strongly increased upon interferon gamma stimulation, leds to the enhanced transcriptional activity of target cytokine genes (By similarity).
Ubiquitination leads to its degradation by the proteasome (PubMed:25122610).
Sumoylated with SUMO3. Desumoylated by SENP1.

Friebus-Kardash, J., Kuang, F., Peitz, T., Hamdan, T. A., Eisenberger, U., Boss, K., ... & Jahn, M. (2023). Expression of Interferon Regulatory Factor 8 (IRF8) and Its Association with Infections in Dialysis Patients. Cells, 12(14), 1892.

Feinberg, P. A., Becker, S. C., Chung, L., Ferrari, L., Stellwagen, D., Anaclet, C., ... & Schafer, D. P. (2022). Elevated TNF-α leads to neural circuit instability in the absence of Interferon Regulatory Factor 8. Journal of Neuroscience, 42(32), 6171-6185.

Wu, H., Li, Y., Shi, G., Du, S., Wang, X., Ye, W., ... & Yang, Y. (2022). Hepatic interferon regulatory factor 8 expression suppresses hepatocellular carcinoma progression and enhances the response to anti–programmed cell death protein‐1 therapy. Hepatology, 76(6), 1602-1616.

Tripathi, A., Singh Rawat, B., Addya, S., Surjit, M., Tailor, P., Vrati, S., & Banerjee, A. (2021). Lack of Interferon (IFN) regulatory factor 8 associated with restricted IFN-γ response augmented Japanese encephalitis virus replication in the mouse brain. Journal of Virology, 95(21), 10-1128.

Shi, G., Zhang, Z., Ma, S., Li, Y., Du, S., Chu, Y., ... & Wu, H. (2021). Hepatic interferon regulatory factor 8 expression mediates liver ischemia/reperfusion injury in mice. Biochemical Pharmacology, 192, 114728.

Xia, X., Wang, W., Yin, K., & Wang, S. (2020). Interferon regulatory factor 8 governs myeloid cell development. Cytokine & Growth Factor Reviews, 55, 48-57.

Twum, D. Y., Colligan, S. H., Hoffend, N. C., Katsuta, E., Gomez, E. C., Hensen, M. L., ... & Abrams, S. I. (2019). IFN regulatory factor–8 expression in macrophages governs an antimetastatic program. Jci Insight, 4(3).

Muhitch, J. B., Hoffend, N. C., Azabdaftari, G., Miller, A., Bshara, W., Morrison, C. D., ... & Abrams, S. I. (2019). Tumor-associated macrophage expression of interferon regulatory Factor-8 (IRF8) is a predictor of progression and patient survival in renal cell carcinoma. Journal for immunotherapy of cancer, 7(1), 1-9.

Clément, M., Haddad, Y., Raffort, J., Lareyre, F., Newland, S. A., Master, L., ... & Mallat, Z. (2018). Deletion of IRF8 (interferon regulatory factor 8)-dependent dendritic cells abrogates proatherogenic adaptive immunity. Circulation research, 122(6), 813-820.

Lv, D. W., Zhang, K., & Li, R. (2018). Interferon regulatory factor 8 regulates caspase-1 expression to facilitate Epstein-Barr virus reactivation in response to B cell receptor stimulation and chemical induction. PLoS pathogens, 14(1), e1006868.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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For research use only. Not intended for any clinical use.

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