Mouse Anti-IRF9 Antibody (2B7) (CBMAB-0554-YC)

Name: Mouse Anti-IRF9 Antibody (2B7)
SKU: CBMAB-0554-YC
Rating: 5 (1 reviews)

See all IRF9 antibodies

Compare Online Inquiry

Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

2B7

Application

IP, MA

Immunogen

Recombinant protein

Specificity

Human

Antibody Isotype

IgG2b

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Supernatant

Storage

Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

More Infomation

Target

Full Name

IRF9

Introduction

IRF9 (interferon regulatory factor 9) is a protein that in humans is encoded by the IRF9 gene, previously known as ISGF3G. IRF9 has been shown to interact with STAT2 and STAT1.

Entrez Gene ID

10379

UniProt ID

Q00978

Alternative Names

p48; IRF-9; ISGF3; ISGF3G

Function

Transcription factor that plays an essential role in anti-viral immunity. It mediates signaling by type I IFNs (IFN-alpha and IFN-beta). Following type I IFN binding to cell surface receptors, Jak kinases (TYK2 and JAK1) are activated, leading to tyrosine phosphorylation of STAT1 and STAT2. IRF9/ISGF3G associates with the phosphorylated STAT1:STAT2 dimer to form a complex termed ISGF3 transcription factor, that enters the nucleus. ISGF3 binds to the IFN stimulated response element (ISRE) to activate the transcription of interferon stimulated genes, which drive the cell in an antiviral state.

Biological Process

Cell surface receptor signaling pathwayManual Assertion Based On ExperimentTAS:ProtInc
Defense response to virusIEA:UniProtKB-KW
Immune system processManual Assertion Based On ExperimentIBA:GO_Central
Positive regulation of transcription by RNA polymerase IIManual Assertion Based On ExperimentIDA:ComplexPortal
Regulation of transcription by RNA polymerase IIManual Assertion Based On ExperimentIBA:GO_Central
Transcription by RNA polymerase IIManual Assertion Based On ExperimentTAS:ProtInc

Cellular Location

Cytoplasm; Nucleus. Translocated into the nucleus upon activation by IFN-alpha/beta.

Involvement in disease

Immunodeficiency 65 (IMD65):
An autosomal recessive immunologic disorder characterized by recurrent viral infections from early infancy. Clinical consequences are pneumonia, bronchiectasis, and septic shock. Affected individuals have lymphopenia or hypogammaglobulinemia, particularly during infection, and impaired cellular type I interferon response. Patients may have adverse response to vaccination with live attenuated vaccines.

PTM

(Microbial infection) Ubiquitinated by Herpes simplex virus 2 E3 ubiquitin ligase ICP22.

Drury, S., Claussen, G., Zetterman, A., Moriyama, H., Moriyama, E. N., & Zhang, L. (2023). Evolution and emergence of primate‐specific interferon regulatory factor 9. Journal of Medical Virology, 95(2), e28521.

Paul, A., Ismail, M. N., Tang, T. H., & Ng, S. K. (2023). Phosphorylation of interferon regulatory factor 9 (IRF9). Molecular biology reports, 50(4), 3909-3917.

Li, L., Fu, J., Li, J., Guo, S., Chen, Q., Zhang, Y., ... & Wang, X. (2022). African swine fever virus pI215L inhibits type I interferon signaling by targeting interferon regulatory factor 9 for Autophagic degradation. Journal of Virology, 96(17), e00944-22.

Gothe, F., Spegarova, J. S., Hatton, C. F., Griffin, H., Sargent, T., Cowley, S. A., ... & Hambleton, S. (2022). Aberrant inflammatory responses to type I interferon in STAT2 or IRF9 deficiency. Journal of Allergy and Clinical Immunology, 150(4), 955-964.

Brunn, D., Turkowski, K., Günther, S., Weigert, A., Muley, T., Kriegsmann, M., ... & Savai, R. (2021). Interferon regulatory factor 9 promotes lung cancer progression via regulation of versican. Cancers, 13(2), 208.

Li, W., Deng, P., Wang, J., Li, Z., & Zhang, H. (2020). MiR-17 knockdown promotes vascular smooth muscle cell phenotypic modulation through upregulated interferon regulator Factor 9 expression. American Journal of Hypertension, 33(12), 1119-1126.

Zhu, Y., Shan, S., Feng, H., Jiang, L., An, L., Yang, G., & Li, H. (2019). Molecular characterization and functional analysis of interferon regulatory factor 9 (irf9) in common carp Cyprinus carpio: a pivotal molecule in the Ifn response against pathogens. Journal of Fish Biology, 95(2), 510-519.

Paul, A., Tang, T. H., & Ng, S. K. (2018). Interferon regulatory factor 9 structure and regulation. Frontiers in immunology, 9, 1831.

Ask a question We look forward to hearing from you.

0 reviews or Q&As

Purchasing FAQs
Technical FAQs

Have you used Mouse Anti-IRF9 Antibody (2B7)?
Submit a review and get a Coupon or an Amazon gift card. 20% off Coupon

$30 eGift Card
Submit a review

Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Related Products

Rabbit Anti-IRF9 Recombinant Antibody (D8G7H) (CAT#: CBMAB-CP1216-LY)

Rabbit Anti-IRF9 Recombinant Antibody (D2T8M) (CAT#: CBMAB-CP1215-LY)

Mouse Anti-IRF9 Recombinant Antibody (CBYY-I0991) (CAT#: CBMAB-I2159-YY)

Mouse Anti-IRF9 Recombinant Antibody (CBYY-I0001) (CAT#: CBMAB-I1493-YY)

Rabbit Anti-IRF9 Recombinant Antibody (D9I5H) (CAT#: CBMAB-CP1220-LY)

Mouse Anti-IRF9 Recombinant Antibody (1C10) (CAT#: CBMAB-I0704-YY)

Mouse Anti-IRF9 Antibody (2F8) (CAT#: CBMAB-0555-YC)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

Online Inquiry

Request bulk or custom quote Second antibody and isotype control

Contact us

Tel: (USA)
(UK)
Fax:

Global Locations

Email:

Online Inquiry