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Mouse Anti-ISCA1 Recombinant Antibody (2E9) (CBMAB-A4558-LY)

The product is antibody recognizes ISCA1. The antibody 2E9 immunoassay techniques such as: WB, ELISA.
See all ISCA1 antibodies

Summary

Host Animal
Mouse
Specificity
Human
Clone
2E9
Antibody Isotype
IgG2a, κ
Application
WB, ELISA

Basic Information

Immunogen
ISCA1 (AAH02675, 1 a.a. ~ 129 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Specificity
Human
Antibody Isotype
IgG2a, κ
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Purity
> 95% Purity determined by SDS-PAGE.
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name
iron-sulfur cluster assembly 1 homolog (S. cerevisiae)
Introduction
ISCA1 is a mitochondrial protein involved in the biogenesis and assembly of iron-sulfur clusters, which play a role in electron-transfer reactions (Cozar-Castellano et al., 2004 [PubMed 15262227]).[supplied by OMIM
Entrez Gene ID
81689
UniProt ID
Q9BUE6
Alternative Names
HBLD2; ISA1; MGC4276; RP11-507D14.2; hIscA
Function
Involved in the maturation of mitochondrial 4Fe-4S proteins functioning late in the iron-sulfur cluster assembly pathway. Probably involved in the binding of an intermediate of Fe/S cluster assembly.
Biological Process
Iron-sulfur cluster assemblyManual Assertion Based On ExperimentIBA:GO_Central
Protein maturation by iron-sulfur cluster transferManual Assertion Based On ExperimentIBA:GO_Central
Cellular Location
Mitochondrion
Involvement in disease
Multiple mitochondrial dysfunctions syndrome 5 (MMDS5):
An autosomal recessive, severe disorder characterized by early onset neurological deterioration, seizures, cerebral and cerebellar leukodystrophy, dysmyelination, cortical migrational abnormalities, lactic acidosis and early demise.

Du, J., Huang, Z., Li, Y., Ren, X., Zhou, C., Liu, R., ... & Tan, G. (2023). Copper exerts cytotoxicity through inhibition of iron-sulfur cluster biogenesis on ISCA1/ISCA2/ISCU assembly proteins. Free Radical Biology and Medicine, 204, 359-373.

Zhu, Y., Huang, H., Chen, Z., Tao, Y., Liao, L. Y., Gao, S. H., ... & Gao, C. Y. (2023). Intermittent Theta Burst Stimulation Attenuates Cognitive Deficits and Alzheimer’s Disease-Type Pathologies via ISCA1-Mediated Mitochondrial Modulation in APP/PS1 Mice. Neuroscience Bulletin, 1-19.

Ling, Y., Yang, X., Zhang, X., Guan, F., Qi, X., Dong, W., ... & Zhang, L. (2022). Myocardium-specific Isca1 knockout causes iron metabolism disorder and myocardial oncosis in rat. Life Sciences, 297, 120485.

Suraci, D., Saudino, G., Nasta, V., Ciofi-Baffoni, S., & Banci, L. (2021). ISCA1 orchestrates ISCA2 and NFU1 in the maturation of human mitochondrial [4Fe-4S] proteins. Journal of Molecular Biology, 433(10), 166924.

Liu, X., Chen, G., He, J., Wan, G., Shen, D., Xia, A., & Chen, F. (2021). Transcriptomic analysis reveals the inhibition of reproduction in rice brown planthopper, Nilaparvata lugens, after silencing the gene of Mag R (IscA1). Insect Molecular Biology, 30(3), 253-263.

Weiler, B. D., Brück, M. C., Kothe, I., Bill, E., Lill, R., & Mühlenhoff, U. (2020). Mitochondrial [4Fe-4S] protein assembly involves reductive [2Fe-2S] cluster fusion on ISCA1–ISCA2 by electron flow from ferredoxin FDX2. Proceedings of the National Academy of Sciences, 117(34), 20555-20565.

Jain, A., Singh, A., Maio, N., & Rouault, T. A. (2020). Assembly of the [4Fe–4S] cluster of NFU1 requires the coordinated donation of two [2Fe–2S] clusters from the scaffold proteins, ISCU2 and ISCA1. Human Molecular Genetics, 29(19), 3165-3182.

Yang, X., Lu, D., Zhang, X., Chen, W., Gao, S., Dong, W., ... & Zhang, L. (2019). Knockout of ISCA 1 causes early embryonic death in rats. Animal Models and Experimental Medicine, 2(1), 18-24.

Shukla, A., Kaur, P., & Girisha, K. M. (2018). Report of the third family with multiple mitochondrial dysfunctions syndrome 5 caused by the founder variant p.(Glu87Lys) in ISCA1. Journal of pediatric genetics, 7(03), 130-133.

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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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