Mouse Anti-IST1 Recombinant Antibody (CBFYH-2598) (CBMAB-H3624-FY)

This product is mouse antibody that recognizes IST1. The antibody CBFYH-2598 can be used for immunoassay techniques such as: ELISA, WB.
See all IST1 antibodies

Datasheet

Summary

Host Animal

Mouse

Specificity

Human

Clone

CBFYH-2598

Antibody Isotype

IgG1

Application

ELISA, WB

Basic Information

Immunogen

Anti-hIST1 recognizes hIST1 expression in H520 whole cell lysate

Specificity

Human

Antibody Isotype

IgG1

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Concentration

1 mg/mL

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name

IST1, ESCRT-III Associated Factor

Introduction

This gene encodes a protein with MIT-interacting motifs that interacts with components of endosomal sorting complexes required for transport (ESCRT). ESCRT functions in vesicle budding, such as that which occurs during membrane abscission in cytokinesis. There is a pseudogene for this gene on chromosome 19. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene.

Entrez Gene ID

9798

UniProt ID

P53990

Alternative Names

IST1, ESCRT-III Associated Factor

Function

ESCRT-III-like protein involved in cytokinesis, nuclear envelope reassembly and endosomal tubulation (PubMed:19129479, PubMed:26040712, PubMed:28242692).
Is required for efficient abscission during cytokinesis (PubMed:19129479).
Involved in recruiting VPS4A and/or VPS4B to the midbody of dividing cells (PubMed:19129480, PubMed:19129479).
During late anaphase, involved in nuclear envelope reassembly and mitotic spindle disassembly together with the ESCRT-III complex: IST1 acts by mediating the recruitment of SPAST to the nuclear membrane, leading to microtubule severing (PubMed:26040712).
Recruited to the reforming nuclear envelope (NE) during anaphase by LEMD2 (PubMed:28242692).
Regulates early endosomal tubulation together with the ESCRT-III complex by mediating the recruitment of SPAST (PubMed:23897888).

Biological Process

AbscissionManual Assertion Based On ExperimentIDA:UniProtKB
Cell divisionManual Assertion Based On ExperimentIMP:UniProtKB
Cytoskeleton-dependent cytokinesisManual Assertion Based On ExperimentIMP:UniProtKB
ESCRT III complex disassembly1 PublicationNAS:ParkinsonsUK-UCL
Establishment of protein localizationManual Assertion Based On ExperimentIMP:UniProtKB
Multivesicular body assembly1 PublicationNAS:ParkinsonsUK-UCL
Positive regulation of collateral sproutingIEA:Ensembl
Positive regulation of proteolysisManual Assertion Based On ExperimentIDA:UniProtKB
Protein localizationManual Assertion Based On ExperimentIMP:UniProtKB
Protein transportIEA:InterPro
Viral capsid secondary envelopmentManual Assertion Based On ExperimentIDA:UniProtKB
Viral release from host cellManual Assertion Based On ExperimentIDA:UniProtKB

Cellular Location

Cytoplasmic vesicle; Cytoplasm, cytoskeleton, microtubule organizing center, centrosome; Midbody; Nucleus envelope. Localizes to centrosome and midbody of dividing cells (PubMed:19129480, PubMed:19129479, PubMed:20719964).
Colocalized with SPART to the ends of Flemming bodies during cytokinesis (PubMed:20719964).
Localizes to the reforming nuclear envelope on chromatin disks during late anaphase

References

Wenzel, D. M., Mackay, D. R., Skalicky, J. J., Paine, E. L., Miller, M. S., Ullman, K. S., & Sundquist, W. I. (2022). Comprehensive analysis of the human ESCRT-III-MIT domain interactome reveals new cofactors for cytokinetic abscission. Elife, 11, e77779.

Merigliano, C., Burla, R., La Torre, M., Del Giudice, S., Teo, H., Liew, C. W., ... & Saggio, I. (2021). AKTIP interacts with ESCRT I and is needed for the recruitment of ESCRT III subunits to the midbody. PLoS Genetics, 17(8), e1009757.

Feng, Q., Luo, Y., Zhang, X. N., Yang, X. F., Hong, X. Y., Sun, D. S., ... & Wang, J. Z. (2020). MAPT/Tau accumulation represses autophagy flux by disrupting IST1-regulated ESCRT-III complex formation: a vicious cycle in Alzheimer neurodegeneration. Autophagy, 16(4), 641-658.

Brune, T., Kunze-Schumacher, H., & Kölling, R. (2019). Interactions in the ESCRT-III network of the yeast Saccharomyces cerevisiae. Current genetics, 65, 607-619.

McCullough, J., Frost, A., & Sundquist, W. I. (2018). Structures, functions, and dynamics of ESCRT-III/Vps4 membrane remodeling and fission complexes. Annual review of cell and developmental biology, 34, 85-109.

Talledge, N., McCullough, J., Wenzel, D., Nguyen, H. C., Lalonde, M. S., Bajorek, M., ... & Sundqust, W. I. (2018). The ESCRT-III proteins IST1 and CHMP1B assemble around nucleic acids. Biorxiv, 386532.

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Protocols

Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Associated Products

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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