Rat Anti-ITGB4 Recombinant Antibody (346-11A) (CBMAB-C1007-LY)

Name: Rat Anti-ITGB4 Recombinant Antibody (346-11A)
SKU: CBMAB-C1007-LY
Rating: 5 (1 reviews)

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Datasheet

SDS

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Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Rat

Clone

346-11A

Application

Specificity

Mouse

Antibody Isotype

IgG2a, κ

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Concentration

0.2 mg/ml

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

More Infomation

Target

Full Name

Integrin Subunit Beta 4

Introduction

Integrins are heterodimers comprised of alpha and beta subunits, that are noncovalently associated transmembrane glycoprotein receptors. Different combinations of alpha and beta polypeptides form complexes that vary in their ligand-binding specificities. Integrins mediate cell-matrix or cell-cell adhesion, and transduced signals that regulate gene expression and cell growth. This gene encodes the integrin beta 4 subunit, a receptor for the laminins. This subunit tends to associate with alpha 6 subunit and is likely to play a pivotal role in the biology of invasive carcinoma. Mutations in this gene are associated with epidermolysis bullosa with pyloric atresia. Multiple alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Entrez Gene ID

192897

UniProt ID

A2A863

Function

Integrin alpha-6/beta-4 is a receptor for laminin. Plays a critical structural role in the hemidesmosome of epithelial cells. Is required for the regulation of keratinocyte polarity and motility. ITGA6:ITGB4 binds to NRG1 (via EGF domain) and this binding is essential for NRG1-ERBB signaling (PubMed:20682778).
ITGA6:ITGB4 binds to IGF1 and this binding is essential for IGF1 signaling (PubMed:22351760).
ITGA6:ITGB4 binds to IGF2 and this binding is essential for IGF2 signaling (PubMed:28873464).

Biological Process

AutophagyManual Assertion Based On ExperimentIMP:UniProtKB
Cell adhesion1 PublicationNAS:ProtInc
Cell adhesion mediated by integrinManual Assertion Based On ExperimentIBA:GO_Central
Cell migrationManual Assertion Based On ExperimentIBA:GO_Central
Cell motilityManual Assertion Based On ExperimentIMP:UniProtKB
Cell-matrix adhesionManual Assertion Based On ExperimentIMP:UniProtKB
Filopodium assemblyIEA:Ensembl
Hemidesmosome assemblyManual Assertion Based On ExperimentIDA:UniProtKB
Integrin-mediated signaling pathwayManual Assertion Based On ExperimentIBA:GO_Central
Mesodermal cell differentiationManual Assertion Based On ExperimentIEP:UniProtKB
Nail developmentManual Assertion Based On ExperimentIMP:UniProtKB
Peripheral nervous system myelin formationIEA:Ensembl
Response to woundingManual Assertion Based On ExperimentIDA:UniProtKB
Skin morphogenesisManual Assertion Based On ExperimentIMP:UniProtKB
Trophoblast cell migrationIEA:Ensembl

Cellular Location

Cell membrane; Cell junction, hemidesmosome. Colocalizes with DST at the leading edge of migrating keratinocytes.

Involvement in disease

Epidermolysis bullosa letalis, with pyloric atresia (EB-PA):
An autosomal recessive, frequently lethal, epidermolysis bullosa with variable involvement of skin, nails, mucosa, and with variable effects on the digestive system. It is characterized by mucocutaneous fragility, aplasia cutis congenita, and gastrointestinal atresia, which most commonly affects the pylorus. Pyloric atresia is a primary manifestation rather than a scarring process secondary to epidermolysis bullosa.
Generalized atrophic benign epidermolysis bullosa (GABEB):
A non-lethal, adult form of junctional epidermolysis bullosa characterized by life-long blistering of the skin, associated with hair and tooth abnormalities.
Epidermolysis bullosa simplex, Weber-Cockayne type (WC-EBS):
A form of intraepidermal epidermolysis bullosa characterized by blistering limited to palmar and plantar areas of the skin.

Topology

Extracellular: 28-710
Helical: 711-733
Cytoplasmic: 734-1822

PTM

Palmitoylated by DHHC3 at several cysteines of the membrane-proximal region, enhancing stability and cell surface expression. Palmitoylation also promotes secondary association with tertaspanins.

Jiang, X., Wang, J., Wang, M., Xuan, M., Han, S., Li, C., ... & Zhao, Z. (2021). ITGB4 as a novel serum diagnosis biomarker and potential therapeutic target for colorectal cancer. Cancer Medicine, 10(19), 6823-6834.

Huang, W., Fan, L., Tang, Y., Chi, Y., & Li, J. (2021). A pan-cancer analysis of the oncogenic role of integrin Beta4 (ITGB4) in human Tumors. International Journal of General Medicine, 9629-9645.

Sung, J. S., Kang, C. W., Kang, S., Jang, Y., Chae, Y. C., Kim, B. G., & Cho, N. H. (2020). ITGB4-mediated metabolic reprogramming of cancer-associated fibroblasts. Oncogene, 39(3), 664-676.

Meng, X., Liu, P., Wu, Y., Liu, X., Huang, Y., Yu, B., ... & Tan, X. (2020). Integrin beta 4 (ITGB4) and its tyrosine-1510 phosphorylation promote pancreatic tumorigenesis and regulate the MEK1-ERK1/2 signaling pathway. Bosnian Journal of Basic Medical Sciences, 20(1), 106.

Wilkinson, E. J., Woodworth, A. M., Parker, M., Phillips, J. L., Malley, R. C., Dickinson, J. L., & Holloway, A. F. (2020). Epigenetic regulation of the ITGB4 gene in prostate cancer. Experimental cell research, 392(2), 112055.

Li, M., Jiang, X., Wang, G., Zhai, C., Liu, Y., Li, H., ... & Zhao, Z. (2019). ITGB4 is a novel prognostic factor in colon cancer. Journal of Cancer, 10(21), 5223.

Wu, P., Wang, Y., Wu, Y., Jia, Z., Song, Y., & Liang, N. (2019). Expression and prognostic analyses of ITGA11, ITGB4 and ITGB8 in human non-small cell lung cancer. PeerJ, 7, e8299.

Yuan, L., Du, X., Tang, S., Wu, S., Wang, L., Xiang, Y., ... & Liu, C. (2019). ITGB 4 deficiency induces senescence of airway epithelial cells through p53 activation. The FEBS Journal, 286(6), 1191-1203.

Hong, D., Zhang, X., Li, R., Yu, J., Lou, Y., He, Q., ... & Chen, Y. (2019). Deletion of TMEM268 inhibits growth of gastric cancer cells by downregulating the ITGB4 signaling pathway. Cell Death & Differentiation, 26(8), 1453-1466.

Liu, C., Yuan, L., Zou, Y., Yang, M., Chen, Y., Qu, X., ... & Qin, X. (2018). ITGB4 is essential for containing HDM-induced airway inflammation and airway hyperresponsiveness. Journal of leukocyte biology, 103(5), 897-908.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
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For research use only. Not intended for any clinical use.

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