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  • Rabbit Anti-JARID2 Recombinant Antibody (CBLXJ-033)

Rabbit Anti-JARID2 Recombinant Antibody (CBLXJ-033) (CBMAB-2194CQ)

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Datasheet
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Basic Information

Host Animal
Rabbit
Clone
CBLXJ-033
Application
WB, IP, ChIP
Specificity
Human, Mouse
Antibody Isotype
IgG
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
More Infomation

Target

Full Name
jumonji and AT-rich interaction domain containing 2
Introduction
This gene encodes a Jumonji- and AT-rich interaction domain (ARID)-domain-containing protein. The encoded protein is a DNA-binding protein that functions as a transcriptional repressor. This protein interacts with the Polycomb repressive complex 2 (PRC2) which plays an essential role in regulating gene expression during embryonic development. This protein facilitates the recruitment of the PRC2 complex to target genes. Alternate splicing results in multiple transcript variants. Mutations in this gene are associated with chronic myeloid malignancies. Diseases associated with JARID2 include Reticuloendotheliosis and Spina Bifida Occulta. Among its related pathways are Mesodermal Commitment Pathway and Gene Expression. An important paralog of this gene is KDM5B.
Entrez Gene ID
Human3720
Mouse16468
UniProt ID
HumanQ92833
MouseQ62315
Alternative Names
JARID2; jumonji, AT rich interactive domain 2; JMJ, jumonji (mouse) homolog, Jumonji, AT rich interactive domain 2; protein Jumonji; jumonji homolog; jumonji-like protein; jumonji/ARID domain-containing protein 2; JMJ
Function
Regulator of histone methyltransferase complexes that plays an essential role in embryonic development, including heart and liver development, neural tube fusion process and hematopoiesis (PubMed:20075857).
Acts as an accessory subunit for the core PRC2 (Polycomb repressive complex 2) complex, which mediates histone H3K27 (H3K27me3) trimethylation on chromatin (PubMed:20075857, PubMed:29499137, PubMed:31959557).
Binds DNA and mediates the recruitment of the PRC2 complex to target genes in embryonic stem cells, thereby playing a key role in stem cell differentiation and normal embryonic development (PubMed:20075857).
In cardiac cells, it is required to repress expression of cyclin-D1 (CCND1) by activating methylation of 'Lys-9' of histone H3 (H3K9me) by the GLP1/EHMT1 and G9a/EHMT2 histone methyltransferases (By similarity).
Also acts as a transcriptional repressor of ANF via its interaction with GATA4 and NKX2-5 (By similarity).
Participates in the negative regulation of cell proliferation signaling (By similarity).
Does not have histone demethylase activity (By similarity).
Biological Process
Cellular response to leukemia inhibitory factorIEA:Ensembl
Central nervous system developmentManual Assertion Based On ExperimentTAS:ProtInc
Chromatin remodelingManual Assertion Based On ExperimentIBA:GO_Central
Dosage compensation by inactivation of X chromosomeIEA:Ensembl
Liver developmentIEA:Ensembl
Negative regulation of cardiac muscle cell proliferationIEA:Ensembl
Negative regulation of cardiac muscle hypertrophyIEA:Ensembl
Negative regulation of histone methylationISS:UniProtKB
Negative regulation of transcription by RNA polymerase IIIEA:Ensembl
Negative regulation of transcription, DNA-templatedISS:UniProtKB
Positive regulation of histone H3-K9 methylationISS:UniProtKB
Protein localization to pericentric heterochromatinIEA:Ensembl
Spleen developmentIEA:Ensembl
Stem cell differentiationISS:UniProtKB
Thymus developmentIEA:Ensembl
Cellular Location
Nucleus. Colocalizes with the PRC2 complex on chromatin.

Verberne, E. A., van der Laan, L., Haghshenas, S., Rooney, K., Levy, M. A., Alders, M., ... & van Haelst, M. M. (2022). DNA methylation signature for JARID2-neurodevelopmental syndrome. International Journal of Molecular Sciences, 23(14), 8001.

Zhang, H., Du, Y., Xin, P., & Man, X. (2022). The LINC00852/miR-29a-3p/JARID2 axis regulates the proliferation and invasion of prostate cancer cell. BMC cancer, 22(1), 1269.

Kasinath, V., Beck, C., Sauer, P., Poepsel, S., Kosmatka, J., Faini, M., ... & Nogales, E. (2021). JARID2 and AEBP2 regulate PRC2 in the presence of H2AK119ub1 and other histone modifications. Science, 371(6527), eabc3393.

Verberne, E. A., Goh, S., England, J., van Ginkel, M., Rafael-Croes, L., Maas, S., ... & Campeau, P. M. (2021). JARID2 haploinsufficiency is associated with a clinically distinct neurodevelopmental syndrome. Genetics in Medicine, 23(2), 374-383.

Al‐Raawi, D., Jones, R., Wijesinghe, S., Halsall, J., Petric, M., Roberts, S., ... & Kanhere, A. (2019). A novel form of JARID2 is required for differentiation in lineage‐committed cells. The EMBO journal, 38(3), e98449.

Wang, X., Lyu, J., Ji, A., Zhang, Q., & Liao, G. (2019). Jarid2 enhances the progression of bladder cancer through regulating PTEN/AKT signaling. Life sciences, 230, 162-168.

Adhikari, A., Mainali, P., & Davie, J. K. (2019). JARID2 and the PRC2 complex regulate the cell cycle in skeletal muscle. Journal of Biological Chemistry, 294(51), 19451-19464.

Celik, H., Koh, W. K., Kramer, A. C., Ostrander, E. L., Mallaney, C., Fisher, D. A., ... & Challen, G. A. (2018). JARID2 functions as a tumor suppressor in myeloid neoplasms by repressing self-renewal in hematopoietic progenitor cells. Cancer cell, 34(5), 741-756.

Kasinath, V., Faini, M., Poepsel, S., Reif, D., Feng, X. A., Stjepanovic, G., ... & Nogales, E. (2018). Structures of human PRC2 with its cofactors AEBP2 and JARID2. Science, 359(6378), 940-944.

Adhikari, A., & Davie, J. (2018). JARID2 and the PRC2 complex regulate skeletal muscle differentiation through regulation of canonical Wnt signaling. Epigenetics & Chromatin, 11, 1-20.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

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For research use only. Not intended for any clinical use.

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