Mouse Anti-KBTBD7 Recombinant Antibody (1G3) (CBMAB-K0461-LY)

This product is antibody recognizes KBTBD7. The antibody 1G3 immunoassay techniques such as: IF, WB.
See all KBTBD7 antibodies

Published Data

Fig.1 Immunoblot analysis of KBTBD7 protein expression in heart tissues of remote area.

Datasheet

Summary

Host Animal

Mouse

Specificity

Human

Clone

1G3

Antibody Isotype

IgG2b

Application

IF, WB

Basic Information

Specificity

Human

Antibody Isotype

IgG2b

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

1% BSA, 50% glycerol

Preservative

0.02% sodium azide

Purity

> 95% Purity determined by SDS-PAGE.

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name

kelch repeat and BTB (POZ) domain containing 7

Introduction

The protein encoded by this gene is a transcriptional activator, having been shown to increase the transcription of activator protein-1 and serum response element. The encoded protein can also form a complex with KBTBD6 and CUL3, which regulates the ubiquitylation and degradation of TIAM1, which is a regulator of RAC1. [provided by RefSeq, Jul 2016]

Entrez Gene ID

84078

UniProt ID

Q8WVZ9

Alternative Names

Kelch Repeat And BTB Domain Containing 7; Kelch Repeat And BTB (POZ) Domain Containing 7;

Function

As part of the CUL3(KBTBD6/7) E3 ubiquitin ligase complex functions as a substrate adapter for the RAC1 guanine exchange factor (GEF) TIAM1, mediating its 'Lys-48' ubiquitination and proteasomal degradation (PubMed:25684205).
By controlling this ubiquitination, regulates RAC1 signal transduction and downstream biological processes including the organization of the cytoskeleton, cell migration and cell proliferation (PubMed:25684205).
Ubiquitination of TIAM1 requires the membrane-associated protein GABARAP which may restrict locally the activity of the complex (PubMed:25684205).

Biological Process

Negative regulation of signal transductionIEA:UniProtKB-KW
Proteasome-mediated ubiquitin-dependent protein catabolic processManual Assertion Based On ExperimentIMP:UniProtKB
Protein K48-linked ubiquitinationManual Assertion Based On ExperimentIDA:UniProtKB
Regulation of Rac protein signal transductionManual Assertion Based On ExperimentIMP:UniProtKB

Cellular Location

Cytoplasm; Nucleus

References

Yuan, C., Chen, Z., & Zhou, Q. (2023). Crocin inhibits KBTBD7 to prevent excessive inflammation and cardiac dysfunction following myocardial infarction. Molecular Medicine Reports, 27(1), 1-11.

Zou, Z., Zhang, B., Li, Z., Lei, L., Sun, G., Jiang, X., ... & Li, Q. (2022). KBTBD7 promotes non‐small cell lung carcinoma progression by enhancing ubiquitin‐dependent degradation of PTEN. Cancer Medicine, 11(23), 4544-4554.

Feng, D., Wang, J., Yang, W., Li, J., Lin, X., Zha, F., ... & Gao, B. (2021). Regulation of Wnt/PCP signaling through p97/VCP-KBTBD7–mediated Vangl ubiquitination and endoplasmic reticulum–associated degradation. Science advances, 7(20), eabg2099.

Song, J., Wu, Q., Jiang, J., Sun, D., Wang, F., Xin, B., & Cui, Q. (2020). Berberine reduces inflammation of human dental pulp fibroblast via miR-21/KBTBD7 axis. Archives of Oral Biology, 110, 104630.

Feng, D. (2020). KBTBD7 regulates Wnt/PCP signaling by targeting Vangl proteins for endoplasmic reticulum-associated degradation via Vangl-p97/VCP direct interaction. HKU Theses Online (HKUTO).

Yang, L., Wang, B., Zhou, Q., Wang, Y., Liu, X., Liu, Z., & Zhan, Z. (2018). MicroRNA-21 prevents excessive inflammation and cardiac dysfunction after myocardial infarction through targeting KBTBD7. Cell death & disease, 9(7), 769.

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Protocols

Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Associated Products

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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