Mouse Anti-KCNC3 Recombinant Antibody (6F7) (CBMAB-K0518-LY)

Name: Mouse Anti-KCNC3 Recombinant Antibody (6F7)
SKU: CBMAB-K0518-LY
Rating: 5 (1 reviews)

See all KCNC3 antibodies

Compare Online Inquiry

Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

6F7

Application

ELISA, WB

Immunogen

KCNC3 (671 a.a. ~ 757 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.Immunogen sequence: ALAHEDCPAI DQPAMSPEDK SPITPGSRGR YSRDRACFLL TDYAPSPDGS IRKATGAPPL PPQDWRKPGP PSFLPDLNAN AAAWISP

Specificity

Human

Antibody Isotype

IgG2b, κ

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Purity

> 95% Purity determined by SDS-PAGE.

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

More Infomation

Target

Full Name

potassium voltage-gated channel, Shaw-related subfamily, member 3

Introduction

The Shaker gene family of Drosophila encodes components of voltage-gated potassium channels and is comprised of four subfamilies. Based on sequence similarity, this gene is similar to one of these subfamilies, namely the Shaw subfamily. The protein encoded by this gene belongs to the delayed rectifier class of channel proteins and is an integral membrane protein that mediates the voltage-dependent potassium ion permeability of excitable membranes. Alternate splicing results in several transcript variants. [provided by RefSeq, Mar 2014]

Entrez Gene ID

3748

UniProt ID

Q14003

Alternative Names

Potassium Voltage-Gated Channel Subfamily C Member 3; Potassium Channel; Voltage Gated Shaw Related Subfamily C; Member 3; Potassium Voltage-Gated Channel; Shaw-Related Subfamily; Member 3; Voltage-Gated Potassium Channel Subunit Kv3.3; KSHIIID; Shaw-Related Voltage-Gated Potassium Channel Protein 3;

Function

Voltage-gated potassium channel that plays an important role in the rapid repolarization of fast-firing brain neurons. The channel opens in response to the voltage difference across the membrane, forming a potassium-selective channel through which potassium ions pass in accordance with their electrochemical gradient. The channel displays rapid activation and inactivation kinetics (PubMed:10712820, PubMed:26997484, PubMed:22289912, PubMed:23734863, PubMed:16501573, PubMed:19953606, PubMed:21479265, PubMed:25756792).
It plays a role in the regulation of the frequency, shape and duration of action potentials in Purkinje cells. Required for normal survival of cerebellar neurons, probably via its role in regulating the duration and frequency of action potentials that in turn regulate the activity of voltage-gated Ca(2+) channels and cellular Ca(2+) homeostasis (By similarity).
Required for normal motor function (PubMed:23734863, PubMed:16501573, PubMed:19953606, PubMed:21479265, PubMed:25756792).
Plays a role in the reorganization of the cortical actin cytoskeleton and the formation of actin veil structures in neuronal growth cones via its interaction with HAX1 and the Arp2/3 complex (PubMed:26997484).

Biological Process

Potassium ion transmembrane transportManual Assertion Based On ExperimentIMP:UniProtKB
Protein homooligomerizationIEA:InterPro
Protein tetramerizationManual Assertion Based On ExperimentIDA:UniProtKB
Regulation of ion transmembrane transportIEA:UniProtKB-KW

Cellular Location

Cell membrane; Cell junction, synapse, presynaptic cell membrane; Perikaryon; Cell projection, axon; Cell projection, dendrite; Cell projection, dendritic spine membrane; Cytoplasm, cell cortex; Cytoplasm, cytoskeleton. Detected on Purkinje cell dendritic spines, positioned perisynaptically but also in extrasynaptic positions along the spine membranes (By similarity).
Detected at presynaptic calices of Held (By similarity).
Colocalizes with the cortical actin cytoskeleton and the Arp2/3 complex (PubMed:26997484).

Involvement in disease

Spinocerebellar ataxia 13 (SCA13):
Spinocerebellar ataxia is a clinically and genetically heterogeneous group of cerebellar disorders. Patients show progressive incoordination of gait and often poor coordination of hands, speech and eye movements, due to degeneration of the cerebellum with variable involvement of the brainstem and spinal cord. SCA13 is an autosomal dominant cerebellar ataxia (ADCA) characterized by slow progression and variable age at onset, ranging from childhood to late adulthood. Mental retardation can be present in some patients.

Topology

Cytoplasmic: 1-290
Helical: 291-309
Extracellular: 351-370
Helical: 371-379
Cytoplasmic: 380-398
Helical: 412-434
Extracellular: 435-447
Helical: 448-469
Helical: 518-539
Cytoplasmic: 540-757

PTM

N-glycosylated.

Li, S., Shang, D., Du, Y., Li, Y., & Liu, R. (2023). Epilepsy as the symptom of a spinocerebellar ataxia 13 in a patient presenting with a mutation in the KCNC3 gene. BMC Neurology, 23(1), 1-5.

Li, Q., Duan, H., Sun, P., Lu, B., Cai, Y., & Shi, W. (2023). KCNC3 acts as a potential biomarker that is involved in the prognosis and tumor immune microenvironment in colorectal cancer.

Xu, P., Shimomura, K., Lee, C., Gao, X., Simpson, E. H., Huang, G., ... & Takahashi, J. S. (2022). A missense mutation in Kcnc3 causes hippocampal learning deficits in mice. Proceedings of the National Academy of Sciences, 119(31), e2204901119.

Sun, L., He, X., Zhang, T., Tao, G., & Wang, X. (2021). Knockdown of lnc-KCNC3-3: 1 alleviates the development of atherosclerosis via downregulation of JAK1/STAT3 signaling pathway. Frontiers in Cardiovascular Medicine, 8, 701058.

Pomarino, D., Thren, J. R., Thren, A., Rostasy, K., & Schoenfeldt, J. (2021). Toe Walking as the Initial Symptom of a Spinocerebellar Ataxia 13 in a Patient Presenting with a Mutation in the KCNC3 Gene. Global Medical Genetics, 9(01), 051-053.

Khare, S., Galeano, K., Zhang, Y., Nick, J. A., Nick, H. S., Subramony, S. H., ... & Waters, M. F. (2018). C-terminal proline deletions in KCNC3 cause delayed channel inactivation and an adult-onset progressive SCA13 with spasticity. The Cerebellum, 17, 692-697.

Ask a question We look forward to hearing from you.

0 reviews or Q&As

Purchasing FAQs
Technical FAQs

Have you used Mouse Anti-KCNC3 Recombinant Antibody (6F7)?
Submit a review and get a Coupon or an Amazon gift card. 20% off Coupon

$30 eGift Card
Submit a review

Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Related Products

Mouse Anti-KCNC3 Recombinant Antibody (1C1) (CAT#: CBMAB-K0516-LY)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

Online Inquiry

Request bulk or custom quote Second antibody and isotype control

Contact us

Tel: (USA)
(UK)
Fax:

Global Locations

Email:

Online Inquiry