Mouse Anti-KCNJ3 Recombinant Antibody (3E11) (CBMAB-K0580-LY)

Name: Mouse Anti-KCNJ3 Recombinant Antibody (3E11)
SKU: CBMAB-K0580-LY
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

3E11

Application

IF, FC, IHC, WB

Immunogen

KCNJ3 antibody was raised in Mouse using Human recombinant protein fragment corresponding to amino acids 279-501 of human KCNJ3 produced in E.coli as the immunogen

Specificity

Human, Monkey, Mouse

Antibody Isotype

IgG2b

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

1% BSA, 50% glycerol

Preservative

0.02% sodium azide

Concentration

0.5-1 mg/ml

Purity

> 95% Purity determined by SDS-PAGE.

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

More Infomation

Target

Full Name

Potassium Voltage-Gated Channel Subfamily J Member 3

Introduction

Potassium channels are present in most mammalian cells, where they participate in a wide range of physiologic responses. The protein encoded by this gene is an integral membrane protein and inward-rectifier type potassium channel. The encoded protein, which has a greater tendency to allow potassium to flow into a cell rather than out of a cell, is controlled by G-proteins and plays an important role in regulating heartbeat. It associates with three other G-protein-activated potassium channels to form a heteromultimeric pore-forming complex that also couples to neurotransmitter receptors in the brain and whereby channel activation can inhibit action potential firing by hyperpolarizing the plasma membrane. These multimeric G-protein-gated inwardly-rectifying potassium (GIRK) channels may play a role in the pathophysiology of epilepsy, addiction, Down's syndrome, ataxia, and Parkinson's disease. Alternative splicing results in multiple transcript variants encoding distinct proteins. [provided by RefSeq, May 2012]

Entrez Gene ID

Human	3760
Mouse	16519
Monkey	696473

UniProt ID

Human	P48549
Mouse	P63250
Monkey	F7ERG6

Alternative Names

Potassium Voltage-Gated Channel Subfamily J Member 3; Potassium Inwardly-Rectifying Channel; Subfamily J; Member 3; Potassium Channel; Inwardly Rectifying Subfamily J Member 3; Inward Rectifier K(+) Channel Kir3.1; GIRK-1; GIRK1;

Function

This potassium channel is controlled by G proteins. Inward rectifier potassium channels are characterized by a greater tendency to allow potassium to flow into the cell rather than out of it. Their voltage dependence is regulated by the concentration of extracellular potassium; as external potassium is raised, the voltage range of the channel opening shifts to more positive voltages. The inward rectification is mainly due to the blockage of outward current by internal magnesium. This receptor plays a crucial role in regulating the heartbeat.

Biological Process

Membrane repolarization during atrial cardiac muscle cell action potential1 PublicationIC:BHF-UCL
Potassium ion import across plasma membraneManual Assertion Based On ExperimentIDA:BHF-UCL
Potassium ion transportManual Assertion Based On ExperimentTAS:ProtInc
Regulation of heart rate by cardiac conduction1 PublicationIC:BHF-UCL
Regulation of ion transmembrane transportManual Assertion Based On ExperimentIBA:GO_Central
Response to electrical stimulusIEA:Ensembl
Ventricular cardiac muscle cell membrane repolarization1 PublicationIC:BHF-UCL

Cellular Location

Membrane

Topology

Cytoplasmic: 1-80
Helical: 81-105
Extracellular: 106-129
Helical: 130-141
Pore-forming: 142-148
Extracellular: 149-157
Helical: 158-179
Cytoplasmic: 180-501

Yamada, N., Asano, Y., Fujita, M., Yamazaki, S., Inanobe, A., Matsuura, N., ... & Takashima, S. (2019). Mutant KCNJ3 and KCNJ5 potassium channels as novel molecular targets in bradyarrhythmias and atrial fibrillation. Circulation, 139(18), 2157-2169.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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