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Mouse Anti-KDELR1 Recombinant Antibody (KR-10) (CBMAB-K0068-LY)

This product is antibody recognizes KDELR1. The antibody KR-10 immunoassay techniques such as: WB, IHC-P, IF/ICC, IP.
See all KDELR1 antibodies
Published Data
Fig.1 Western blot analyses were performed with an anti-dFUS antibody using proteins extracted with Low Salt buffer (LS), 2% N-Lauroylsarcosine-containing buffer (SARK), and 8 M Urea-containing buffer (UREA) from the adult heads of 6-day-old flies developed at 28 °C.
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Fig.2 Overexpression of dPARP16 leads to a strong re-arrangement of the ER marked by calnexin that appears to accumulate at the nuclear envelop (arrows) and KDEL receptor that appears to concentrate near the cell cortex.
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Summary

Host Animal
Mouse
Specificity
Cattle, Human, Mouse, Rat, Rabbit, Chicken, Monkey, Pig, Sheep, Hamster, Frog
Clone
KR-10
Antibody Isotype
IgG1
Application
WB, IHC-P, IF/ICC, IP

Basic Information

Immunogen
A 21 residue synthetic peptide based on the bovine KDEL receptor and the peptide coupled to KLH
Specificity
Cattle, Human, Mouse, Rat, Rabbit, Chicken, Monkey, Pig, Sheep, Hamster, Frog
Antibody Isotype
IgG1
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
50% Glycerol
Preservative
0.09% sodium azide
Purity
> 95% Purity determined by SDS-PAGE.
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name
KDEL Endoplasmic Reticulum Protein Retention Receptor 1
Introduction
Retention of resident soluble proteins in the lumen of the endoplasmic reticulum (ER) is achieved in both yeast and animal cells by their continual retrieval from the cis-Golgi, or a pre-Golgi compartment. Sorting of these proteins is dependent on a C-terminal tetrapeptide signal, usually lys-asp-glu-leu (KDEL) in animal cells, and his-asp-glu-leu (HDEL) in S. cerevisiae. This process is mediated by a receptor that recognizes, and binds the tetrapeptide-containing protein, and returns it to the ER. In yeast, the sorting receptor encoded by a single gene, ERD2, which is a seven-transmembrane protein. Unlike yeast, several human homologs of the ERD2 gene, constituting the KDEL receptor gene family, have been described. The protein encoded by this gene was the first member of the family to be identified, and it encodes a protein structurally and functionally similar to the yeast ERD2 gene product. [provided by RefSeq, Jul 2008]
Entrez Gene ID
Human10945
Mouse68137
Rat361577
Cattle618184
Rabbit100349892
Monkey717928
Pig100520962
Sheep101122047
Hamster100750912
Frog395012
UniProt ID
HumanP24390
MouseQ99JH8
RatQ569A6
CattleP33946
MonkeyA0A0D9S2T7
PigF2Z4Z3
SheepW5PQ26
HamsterA0A2Z5WAD6
FrogQ5XHA2
Function
Receptor for the C-terminal sequence motif K-D-E-L that is present on endoplasmic reticulum resident proteins and that mediates their recycling from the Golgi back to the endoplasmic reticulum.
Biological Process
Endoplasmic reticulum to Golgi vesicle-mediated transportManual Assertion Based On ExperimentIBA:GO_Central
Protein retention in ER lumenManual Assertion Based On ExperimentIBA:GO_Central
Protein transportIEA:UniProtKB-KW
Retrograde vesicle-mediated transport, Golgi to endoplasmic reticulumManual Assertion Based On ExperimentIDA:UniProtKB
T cell apoptotic processIEA:Ensembl
T cell differentiationIEA:Ensembl
Cellular Location
Golgi apparatus membrane; Cytoplasmic vesicle, COPI-coated vesicle membrane; Endoplasmic reticulum membrane; Endoplasmic reticulum-Golgi intermediate compartment membrane. Localized in the Golgi in the absence of bound proteins with the sequence motif K-D-E-L. Trafficks back to the endoplasmic reticulum together with cargo proteins containing the sequence motif K-D-E-L.
Topology
Lumenal: 1-4
Helical: 5-24
Cytoplasmic: 25-32
Helical: 33-52
Lumenal: 53-58
Helical: 59-79
Cytoplasmic: 80-92
Helical: 93-110
Lumenal: 111-116
Helical: 117-135
Cytoplasmic: 136-149
Helical: 150-168
Lumenal: 169-178
Helical: 179-199
Cytoplasmic: 200-212
PTM
Phosphorylation by PKA at Ser-209 is required for endoplasmic reticulum retention function.
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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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