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Mouse Anti-KDM3A Recombinant Antibody (1E12) (CBMAB-0094-WJ)

This product is a mouse antibody that recognizes KDM3A. The antibody 1E12 can be used for immunoassay techniques such as: IHC, WB.
See all KDM3A antibodies

Summary

Host Animal
Mouse
Specificity
Human
Clone
1E12
Antibody Isotype
IgG1
Application
IHC, WB

Basic Information

Immunogen
Purified recombinant fragment of human KDM3A.
Specificity
Human
Antibody Isotype
IgG1
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
Lysine Demethylase 3A
Introduction
KDM3A (Lysine Demethylase 3A) encodes a zinc finger protein that contains a jumonji domain and may play a role in hormone-dependent transcriptional activation. Among its related pathways are Coregulation of Androgen receptor activity and Chromatin organization. An important paralog of this gene is KDM3B.
Entrez Gene ID
UniProt ID
Alternative Names
TSGA; JMJD1; JHDM2A; JHMD2A; JMJD1A
Function
Histone demethylase that specifically demethylates 'Lys-9' of histone H3, thereby playing a central role in histone code. Preferentially demethylates mono- and dimethylated H3 'Lys-9' residue, with a preference for dimethylated residue, while it has weak or no activity on trimethylated H3 'Lys-9'. Demethylation of Lys residue generates formaldehyde and succinate. Involved in hormone-dependent transcriptional activation, by participating in recruitment to androgen-receptor target genes, resulting in H3 'Lys-9' demethylation and transcriptional activation. Involved in spermatogenesis by regulating expression of target genes such as PRM1 and TNP1 which are required for packaging and condensation of sperm chromatin. Involved in obesity resistance through regulation of metabolic genes such as PPARA and UCP1.
Biological Process
Androgen receptor signaling pathwayManual Assertion Based On ExperimentIDA:UniProtKB
Cellular response to leukemia inhibitory factorIEA:Ensembl
Chromatin organizationIEA:UniProtKB-KW
Formaldehyde biosynthetic processManual Assertion Based On ExperimentIDA:UniProtKB
Histone H3-K9 demethylationManual Assertion Based On ExperimentIDA:UniProtKB
Histone H3-K9 dimethylationIEA:Ensembl
Hormone-mediated signaling pathwayManual Assertion Based On ExperimentIDA:UniProtKB
Negative regulation of histone H3-K9 methylationIEA:Ensembl
Positive regulation of cold-induced thermogenesisBy SimilarityISS:YuBioLab
Positive regulation of transcription by RNA polymerase IIIEA:Ensembl
Positive regulation of transcription, DNA-templatedManual Assertion Based On ExperimentIMP:UniProtKB
Regulation of stem cell differentiationIEA:Ensembl
Regulation of stem cell population maintenanceIEA:Ensembl
Regulation of transcription by RNA polymerase IIManual Assertion Based On ExperimentIBA:GO_Central
Spermatid nucleus elongationIEA:Ensembl
Cellular Location
Cytoplasm; Nucleus. Nuclear in round spermatids. When spermatids start to elongate, localizes to the cytoplasm where it forms distinct foci which disappear in mature spermatozoa (By similarity).

Zhang, W., Liu, R., Zhang, L., Wang, C., Dong, Z., Feng, J., ... & Wei, Q. (2022). Downregulation of miR-335 exhibited an oncogenic effect via promoting KDM3A/YAP1 networks in clear cell renal cell carcinoma. Cancer Gene Therapy, 29(5), 573-584.

Hou, X., Li, Q., Yang, L., Yang, Z., He, J., Li, Q., & Li, D. (2021). KDM1A and KDM3A promote tumor growth by upregulating cell cycle-associated genes in pancreatic cancer. Experimental Biology and Medicine, 246(17), 1869-1883.

Zhu, Z., Wu, X., Li, Q., Zhang, J., Yu, S., Shen, Q., ... & Hua, J. (2021). Histone demethylase complexes KDM3A and KDM3B cooperate with OCT4/SOX2 to define a pluripotency gene regulatory network. The FASEB Journal, 35(6), e21664.

Yoo, J., Jeon, Y. H., Cho, H. Y., Lee, S. W., Kim, G. W., Lee, D. H., & Kwon, S. H. (2020). Advances in histone demethylase KDM3A as a cancer therapeutic target. Cancers, 12(5), 1098.

Macedo-Silva, C., Miranda-Gonçalves, V., Lameirinhas, A., Lencart, J., Pereira, A., Lobo, J., ... & Jerónimo, C. (2020). JmjC-KDMs KDM3A and KDM6B modulate radioresistance under hypoxic conditions in esophageal squamous cell carcinoma. Cell Death & Disease, 11(12), 1068.

Wang, H. Y., Long, Q. Y., Tang, S. B., Xiao, Q., Gao, C., Zhao, Q. Y., ... & Wu, M. (2019). Histone demethylase KDM3A is required for enhancer activation of hippo target genes in colorectal cancer. Nucleic Acids Research, 47(5), 2349-2364.

Qian, X., Li, X., Shi, Z., Bai, X., Xia, Y., Zheng, Y., ... & Lu, Z. (2019). KDM3A senses oxygen availability to regulate PGC-1α-mediated mitochondrial biogenesis. Molecular cell, 76(6), 885-895.

Liu, J., Liang, T., & Zhangsun, W. (2019). KDM3A is associated with tumor metastasis and modulates colorectal cancer cell migration and invasion. International journal of biological macromolecules, 126, 318-325.

Jones, D., Wilson, L., Thomas, H., Gaughan, L., & Wade, M. A. (2019). The histone demethylase enzymes KDM3A and KDM4B co-operatively regulate chromatin transactions of the estrogen receptor in breast cancer. Cancers, 11(8), 1122.

Kim, H., Kim, D., Choi, S. A., Kim, C. R., Oh, S. K., Pyo, K. E., ... & Baek, S. H. (2018). KDM3A histone demethylase functions as an essential factor for activation of JAK2− STAT3 signaling pathway. Proceedings of the National Academy of Sciences, 115(46), 11766-11771.

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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

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