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Mouse Anti-KDM4A Recombinant Antibody (13D6) (CBMAB-K0686-LY)

This product is antibody recognizes KDM4A. The antibody 13D6 immunoassay techniques such as: WB, IP, ELISA, FC, IHC-P, ICC/IF.
See all KDM4A antibodies
Published Data
Fig.1 Western blotting carried out using anti-KDM4A and anti-KDM4C antibodies in sorted FLK1/VE-cadherin-double-positive cells derived from mESCs at day 6 of differentiation.
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Fig.2 Flow cytometry assessment of cell surface expression of Flk1 and VE-cadherin on day 6 of differentiation following treatment with scrambled, Kdm4a.
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Fig.3 Unsorted differentiated cells were suspended in Matrigel containing VEGF and injected subcutaneous into nude mice. Matrigel plugs were harvested on day 7 and assessed by H&E staining.
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Fig.4 Immunostaining was performed using anti-CD31 and anti-VE-cadherin antibodies.
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Summary

Host Animal
Mouse
Specificity
Mouse, Human
Clone
13D6
Antibody Isotype
IgG
Application
WB, IP, ELISA, FC, IHC-P, ICC/IF

Basic Information

Specificity
Mouse, Human
Antibody Isotype
IgG
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Concentration
1 mg/mL
Purity
> 95% Purity determined by SDS-PAGE.
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name
Lysine Demethylase 4A
Introduction
This gene is a member of the Jumonji domain 2 (JMJD2) family and encodes a protein containing a JmjN domain, a JmjC domain, a JD2H domain, two TUDOR domains, and two PHD-type zinc fingers. This nuclear protein functions as a trimethylation-specific demethylase, converting specific trimethylated histone residues to the dimethylated form, and as a transcriptional repressor. [provided by RefSeq, Apr 2009]
Entrez Gene ID
Human9682
Mouse230674
UniProt ID
HumanO75164
MouseQ8BW72
Function
Histone demethylase that specifically demethylates 'Lys-9' and 'Lys-36' residues of histone H3, thereby playing a central role in histone code (PubMed:26741168).
Does not demethylate histone H3 'Lys-4', H3 'Lys-27' nor H4 'Lys-20'. Demethylates trimethylated H3 'Lys-9' and H3 'Lys-36' residue, while it has no activity on mono- and dimethylated residues. Demethylation of Lys residue generates formaldehyde and succinate. Participates in transcriptional repression of ASCL2 and E2F-responsive promoters via the recruitment of histone deacetylases and NCOR1, respectively3 Publications
Isoform 2
Crucial for muscle differentiation, promotes transcriptional activation of the Myog gene by directing the removal of repressive chromatin marks at its promoter. Lacks the N-terminal demethylase domain.
Biological Process
Cardiac muscle hypertrophy in response to stressIEA:Ensembl
Chromatin remodelingManual Assertion Based On ExperimentIBA:GO_Central
Histone demethylationManual Assertion Based On ExperimentIDA:UniProtKB
Histone H3-K36 demethylationManual Assertion Based On ExperimentIDA:UniProtKB
Histone H3-K9 demethylationManual Assertion Based On ExperimentIMP:UniProtKB
Negative regulation of astrocyte differentiationIEA:Ensembl
Negative regulation of autophagyManual Assertion Based On ExperimentIMP:ParkinsonsUK-UCL
Negative regulation of cell deathIEA:Ensembl
Negative regulation of gene expressionManual Assertion Based On ExperimentIMP:ParkinsonsUK-UCL
Negative regulation of histone H3-K9 trimethylationIEA:Ensembl
Negative regulation of transcription, DNA-templatedManual Assertion Based On ExperimentIDA:UniProtKB
Positive regulation of gene expressionIEA:Ensembl
Positive regulation of neuron differentiationIEA:Ensembl
Response to nutrient levelsIEA:Ensembl
Cellular Location
Nucleus
PTM
Ubiquitinated by RNF8 and RNF168 following DNA damage, leading to its degradation. Degradation promotes accessibility of H4K20me2 mark for DNA repair protein TP53BP1, which is then recruited.
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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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