Rabbit Anti-KDM4A Recombinant Antibody (C37E5) (CBMAB-CP1268-LY)

Name: Rabbit Anti-KDM4A Recombinant Antibody (C37E5)
SKU: CBMAB-CP1268-LY
Rating: 5 (1 reviews)

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Datasheet

SDS

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Basic Information

Host Animal

Rabbit

Clone

C37E5

Application

WB, IP, IHC-P, IF (ICC)

Immunogen

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Val444 of human JMJD2A protein.

Specificity

Human

Antibody Isotype

IgG

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

100 µg/ml BSA, 50% glycerol

Preservative

0.02% sodium azide

Purity

> 95% Purity determined by SDS-PAGE.

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

More Infomation

Target

Full Name

Lysine Demethylase 4A

Introduction

This gene is a member of the Jumonji domain 2 (JMJD2) family and encodes a protein containing a JmjN domain, a JmjC domain, a JD2H domain, two TUDOR domains, and two PHD-type zinc fingers. This nuclear protein functions as a trimethylation-specific demethylase, converting specific trimethylated histone residues to the dimethylated form, and as a transcriptional repressor. [provided by RefSeq, Apr 2009]

Entrez Gene ID

9682

UniProt ID

O75164

Alternative Names

Lysine Demethylase 4A; JmjC Domain-Containing Histone Demethylation Protein 3A; Jumonji C Domain-Containing Histone Demethylase 3A; Jumonji Domain-Containing Protein 2A; Lysine (K)-Specific Demethylase 4A; Jumonji Domain Containing 2A; Tudor Domain Containing 14A; Jumonji Domain Containing 2; JMJD2A;

Function

Histone demethylase that specifically demethylates 'Lys-9' and 'Lys-36' residues of histone H3, thereby playing a central role in histone code (PubMed:26741168).
Does not demethylate histone H3 'Lys-4', H3 'Lys-27' nor H4 'Lys-20'. Demethylates trimethylated H3 'Lys-9' and H3 'Lys-36' residue, while it has no activity on mono- and dimethylated residues. Demethylation of Lys residue generates formaldehyde and succinate. Participates in transcriptional repression of ASCL2 and E2F-responsive promoters via the recruitment of histone deacetylases and NCOR1, respectively3 Publications
Isoform 2
Crucial for muscle differentiation, promotes transcriptional activation of the Myog gene by directing the removal of repressive chromatin marks at its promoter. Lacks the N-terminal demethylase domain.

Biological Process

Cardiac muscle hypertrophy in response to stressIEA:Ensembl
Chromatin remodelingManual Assertion Based On ExperimentIBA:GO_Central
Histone demethylationManual Assertion Based On ExperimentIDA:UniProtKB
Histone H3-K36 demethylationManual Assertion Based On ExperimentIDA:UniProtKB
Histone H3-K9 demethylationManual Assertion Based On ExperimentIMP:UniProtKB
Negative regulation of astrocyte differentiationIEA:Ensembl
Negative regulation of autophagyManual Assertion Based On ExperimentIMP:ParkinsonsUK-UCL
Negative regulation of cell deathIEA:Ensembl
Negative regulation of gene expressionManual Assertion Based On ExperimentIMP:ParkinsonsUK-UCL
Negative regulation of histone H3-K9 trimethylationIEA:Ensembl
Negative regulation of transcription, DNA-templatedManual Assertion Based On ExperimentIDA:UniProtKB
Positive regulation of gene expressionIEA:Ensembl
Positive regulation of neuron differentiationIEA:Ensembl
Response to nutrient levelsIEA:Ensembl

Cellular Location

Nucleus

PTM

Ubiquitinated by RNF8 and RNF168 following DNA damage, leading to its degradation. Degradation promotes accessibility of H4K20me2 mark for DNA repair protein TP53BP1, which is then recruited.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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