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Mouse Anti-KDM5A Recombinant Antibody (1H2) (CBMAB-A4616-LY)

The product is antibody recognizes JARID1A. The antibody 1H2 immunoassay techniques such as: WB, ELISA.
See all KDM5A antibodies
Published Data
Fig.1 Evaluation of GC-1 cells over expressing Kdm5a.
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Fig.2 GC-1 cells were transfected with control, Gfp and Kdm5aþGfp expression vectors.
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Summary

Host Animal
Mouse
Specificity
Human
Clone
1H2
Antibody Isotype
IgG2a, κ
Application
WB, ELISA

Basic Information

Immunogen
JARID1A (NP_005047, 191 a.a. ~ 290 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Specificity
Human
Antibody Isotype
IgG2a, κ
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Purity
> 95% Purity determined by SDS-PAGE.
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name
Lysine Demethylase 5A
Introduction
The protein encoded by this gene is a ubiquitously expressed nuclear protein. It binds directly, with several other proteins, to retinoblastoma protein which regulates cell proliferation. This protein also interacts with rhombotin-2 which functions distinctly in erythropoiesis and in T-cell leukemogenesis. Rhombotin-2 is thought to either directly affect the activity of the encoded protein or may indirectly modulate the functions of the retinoblastoma protein by binding to this protein. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq]
Entrez Gene ID
5927
UniProt ID
P29375
Alternative Names
KDM5A; RBBP2; RBP2
Function
Histone demethylase that specifically demethylates 'Lys-4' of histone H3, thereby playing a central role in histone code. Does not demethylate histone H3 'Lys-9', H3 'Lys-27', H3 'Lys-36', H3 'Lys-79' or H4 'Lys-20'. Demethylates trimethylated and dimethylated but not monomethylated H3 'Lys-4'. Regulates specific gene transcription through DNA-binding on 5'-CCGCCC-3' motif (PubMed:18270511).
May stimulate transcription mediated by nuclear receptors. Involved in transcriptional regulation of Hox proteins during cell differentiation (PubMed:19430464).
May participate in transcriptional repression of cytokines such as CXCL12. Plays a role in the regulation of the circadian rhythm and in maintaining the normal periodicity of the circadian clock. In a histone demethylase-independent manner, acts as a coactivator of the CLOCK-ARNTL/BMAL1-mediated transcriptional activation of PER1/2 and other clock-controlled genes and increases histone acetylation at PER1/2 promoters by inhibiting the activity of HDAC1 (By similarity).
Seems to act as a transcriptional corepressor for some genes such as MT1F and to favor the proliferation of cancer cells (PubMed:27427228).
Biological Process
Chromatin remodelingManual Assertion Based On ExperimentIBA:GO_Central
Circadian regulation of gene expressionISS:UniProtKB
Histone H3-K4 demethylationManual Assertion Based On ExperimentIDA:CAFA
Negative regulation of histone deacetylase activityISS:UniProtKB
Negative regulation of transcription by RNA polymerase IIIEA:Ensembl
Positive regulation of transcription, DNA-templatedManual Assertion Based On ExperimentIDA:UniProtKB
Regulation of DNA-binding transcription factor activityManual Assertion Based On ExperimentIMP:CAFA
Cellular Location
Nucleus, nucleolus. Occupies promoters of genes involved in RNA metabolism and mitochondrial function.
Involvement in disease
A chromosomal aberration involving KDM5A has been found in M5 type acute myeloid leukemia. Translocation t(11;12)(p15;p13) with NUP98.
Chromosomal aberrations involving KDM5A have been found in M7 type childhood acute myeloid leukemia. Translocation t(11;12)(p15;p13) with NUP98

Xu, S., Wang, S., Xing, S., Yu, D., Rong, B., Gao, H., ... & Lan, F. (2021). KDM5A suppresses PML-RARα target gene expression and APL differentiation through repressing H3K4me2. Blood Advances, 5(17), 3241-3253.

Noort, S., Wander, P., Alonzo, T. A., Smith, J., Ries, R. E., Gerbing, R. B., ... & Meshinchi, S. (2021). The clinical and biological characteristics of NUP98-KDM5A pediatric acute myeloid leukemia. Haematologica, 106(2), 630.

Gaillard, S., Charasson, V., Ribeyre, C., Salifou, K., Pillaire, M. J., Hoffmann, J. S., ... & Vandromme, M. (2021). KDM5A and KDM5B histone-demethylases contribute to HU-induced replication stress response and tolerance. Biology Open, 10(5), bio057729.

Yang, G. J., Zhu, M. H., Lu, X. J., Liu, Y. J., Lu, J. F., Leung, C. H., ... & Chen, J. (2021). The emerging role of KDM5A in human cancer. Journal of hematology & oncology, 14(1), 1-18.

Yang, G. J., Wu, J., Miao, L., Zhu, M. H., Zhou, Q. J., Lu, X. J., ... & Chen, J. (2021). Pharmacological inhibition of KDM5A for cancer treatment. European Journal of Medicinal Chemistry, 226, 113855.

Kirtana, R., Manna, S., & Patra, S. K. (2020). Molecular mechanisms of KDM5A in cellular functions: Facets during development and disease. Experimental Cell Research, 396(2), 112314.

Wang, L., Gao, Y., Zhang, G., Li, D., Wang, Z., Zhang, J., ... & Liao, X. (2020). Enhancing KDM5A and TLR activity improves the response to immune checkpoint blockade. Science Translational Medicine, 12(560), eaax2282.

Yang, G. J., Ko, C. N., Zhong, H. J., Leung, C. H., & Ma, D. L. (2019). Structure-based discovery of a selective KDM5A inhibitor that exhibits anti-cancer activity via inducing cell cycle arrest and senescence in breast cancer cell lines. Cancers, 11(1), 92.

Mitsui, E., Yoshida, S., Shinoda, Y., Matsumori, Y., Tsujii, H., Tsuchida, M., ... & Mizukami, T. (2019). Identification of ryuvidine as a KDM5A inhibitor. Scientific Reports, 9(1), 9952.

Shokri, G., Doudi, S., Fathi-Roudsari, M., Kouhkan, F., & Sanati, M. H. (2018). Targeting histone demethylases KDM5A and KDM5B in AML cancer cells: a comparative view. Leukemia research, 68, 105-111.

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For research use only. Not intended for any clinical use.

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