Mouse Anti-NCAPD3 Recombinant Antibody (1D5) (CBMAB-A4674-LY)

The product is antibody recognizes KIAA0056. The antibody 1D5 immunoassay techniques such as: WB, ELISA.
See all NCAPD3 antibodies

Datasheet

Summary

Host Animal

Mouse

Specificity

Human

Clone

1D5

Antibody Isotype

IgG1, κ

Application

WB, ELISA

Basic Information

Immunogen

KIAA0056 (AAH11408.1, 1 a.a. ~ 341 a.a) full length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.

Specificity

Human

Antibody Isotype

IgG1, κ

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Purity

> 95% Purity determined by SDS-PAGE.

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name

Non-SMC Condensin II Complex Subunit D3

Introduction

Condensin complexes I and II play essential roles in mitotic chromosome assembly and segregation. Both condensins contain 2 invariant structural maintenance of chromosome (SMC) subunits, SMC2 (MIM 605576) and SMC4 (MIM 605575), but they contain different sets of non-SMC subunits. NCAPD3 is 1 of 3 non-SMC subunits that define condensin II (Ono et al., 2003 [PubMed 14532007]).[supplied by OMIM

Entrez Gene ID

23310

UniProt ID

P42695

Alternative Names

CAP-D3; FLJ42888; KIAA0056; MGC104671; hCAP-D3; hHCP-6; hcp-6

Function

Regulatory subunit of the condensin-2 complex, a complex which establishes mitotic chromosome architecture and is involved in physical rigidity of the chromatid axis (PubMed:14532007).

May promote the resolution of double-strand DNA catenanes (intertwines) between sister chromatids. Condensin-mediated compaction likely increases tension in catenated sister chromatids, providing directionality for type II topoisomerase-mediated strand exchanges toward chromatid decatenation. Specifically required for decatenation of centromeric ultrafine DNA bridges during anaphase. Early in neurogenesis, may play an essential role to ensure accurate mitotic chromosome condensation in neuron stem cells, ultimately affecting neuron pool and cortex size (PubMed:27737959).

Biological Process

Cell division Source: UniProtKB-KW
Meiotic chromosome condensation Source: GO_Central
Mitotic chromosome condensation Source: UniProtKB

Cellular Location

Nucleus

Involvement in disease

Microcephaly 22, primary, autosomal recessive (MCPH22):
A form of microcephaly, a disease defined as a head circumference more than 3 standard deviations below the age, sex and ethnically matched mean. Brain weight is markedly reduced and the cerebral cortex is disproportionately small.

References

Lu, T., Zhou, X., Yang, J., Cai, Y., Ding, M., Chen, X., ... & Wang, X. (2023). NCAPD3 Prompts Diffuse Large B-Cell Lymphoma Progression through Modulating SIRT1 Expression in H3K9 Monomethylation-Dependent Manner. Blood, 142, 4363.

Jing, Z., Liu, Q., Xie, W., Wei, Y., Liu, J., Zhang, Y., ... & Liu, P. (2022). NCAPD3 promotes prostate cancer progression by up-regulating EZH2 and MALAT1 through STAT3 and E2F1. Cellular signalling, 92, 110265.

Yin, Y., Liu, Q., Shao, Y., He, X., Zhu, Q., Lu, S., & Liu, P. (2022). Regulatory mechanism of androgen receptor on NCAPD3 gene expression in prostate cancer. The Prostate, 82(1), 26-40.

Jing, Z., Liu, Q., He, X., Jia, Z., Xu, Z., Yang, B., & Liu, P. (2022). NCAPD3 enhances Warburg effect through c-myc and E2F1 and promotes the occurrence and progression of colorectal cancer. Journal of Experimental & Clinical Cancer Research, 41(1), 1-18.

Kheirkhah, S., Javanzad, M., Hoseinzadeh, M., Mehrabani, Z. H. A., Mohammadzadeh, N., & Monfaredan, A. (2020). Monitoring prostate cancer (PCa) with appraise the gene expression of PRUNE2, NCAPD3 and ASPA and their connection with age, family history and tumor stage. Gene Reports, 21, 100840.

Yang, J., Li, Y., Zhang, Y., Zhou, X., Zhao, Y., Han, Y., ... & Wang, X. (2019). NCAPD3 Regulates Tumor Growth and Predicts Prognosis in Diffuse Large B-Cell Lymphoma. Blood, 134, 5045.

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Protocols

Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Associated Products

Alternative Versions

Mouse Anti-NCAPD3 Recombinant Antibody (1D5)

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Mouse Anti-NCAPD3 Recombinant Antibody (1D5)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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