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Rabbit Anti-KLF1 Recombinant Antibody (EG1799) (CBMAB-EN2147-LY)

The product is antibody recognizes KLF. The antibody EG1799 immunoassay techniques such as: WB: 1:500~1:1000 ELISA: 1:20000.
See all KLF1 antibodies

Summary

Host Animal
Rabbit
Specificity
Human, Mouse, Rat
Clone
EG1799
Antibody Isotype
IgG
Application
WB: 1:500~1:1000 ELISA: 1:20000

Basic Information

Immunogen
The antibody was produced against synthesized peptide derived from internal of human KLF.
Specificity
Human, Mouse, Rat
Antibody Isotype
IgG
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name
Kruppel like factor 15
Introduction
This gene encodes a hematopoietic-specific transcription factor that induces high-level expression of adult beta-globin and other erythroid genes. The zinc-finger protein binds to the DNA sequence CCACACCCT found in the beta hemoglobin promoter. Heterozygous loss-of-function mutations in this gene result in the dominant In(Lu) blood phenotype.
Entrez Gene ID
Human10661
Mouse16596
Rat304666
UniProt ID
HumanQ13351
MouseP46099
RatG3V6F3
Alternative Names
EKLF; EKLF/KLF1
Function
ranscription regulator of erythrocyte development that probably serves as a general switch factor during erythropoiesis. Is a dual regulator of fetal-to-adult globin switching. Binds to the CACCC box in the beta-globin gene promoter and acts as a preferential activator of this gene. Furthermore, it binds to the BCL11A promoter and activates expression of BCL11A, which in turn represses the HBG1 and HBG2 genes. This dual activity ensures that, in most adults, fetal hemoglobin levels are low. Able to activate CD44 and AQP1 promoters. When sumoylated, acts as a transcriptional repressor by promoting interaction with CDH2/MI2beta and also represses megakaryocytic differentiation.
Biological Process
Cellular response to peptideIEA:Ensembl
Erythrocyte differentiationManual Assertion Based On ExperimentIMP:UniProtKB
Maternal process involved in female pregnancyIEA:Ensembl
Positive regulation of transcription, DNA-templatedManual Assertion Based On ExperimentIDA:UniProtKB
Regulation of transcription by RNA polymerase IIManual Assertion Based On ExperimentIBA:GO_Central
Regulation of transcription, DNA-templatedManual Assertion Based On ExperimentIMP:UniProtKB
Cellular Location
Nucleus. Colocalizes with SUMO1 in nuclear speckles.
Involvement in disease
Anemia, congenital dyserythropoietic, 4 (CDAN4):
A blood disorder characterized by ineffective erythropoiesis and hemolysis resulting in anemia. Circulating erythroblasts and erythroblasts in the bone marrow show various morphologic abnormalities. Affected individuals with CDA4 also have increased levels of fetal hemoglobin.
PTM
Acetylated; can be acetylated on both Lys-274 and Lys-288. Acetylation on Lys-274 (by CBP) appears to be the major site affecting EKLF transactivation activity (By similarity).
Sumoylated; sumoylation, promoted by PIAS1, leads to repression of megakaryocyte differentiation. Also promotes the interaction with the CDH4 subunit of the NuRD repression complex (By similarity).
Phosphorylated primarily on serine residues in the transactivation domain. Phosphorylation on Thr-23 is critical for the transactivation activity (By similarity).

Suzuki, H., Ogawa, T., Fujita, S., Sone, R., & Kawahara, A. (2023). Cooperative contributions of the klf1 and klf17 genes in zebrafish primitive erythropoiesis. Scientific Reports, 13(1), 12279.

Hermeling, J. C., Herholz, M., Baumann, L., Cores, E. C., Zečić, A., Hoppe, T., ... & Trifunovic, A. (2022). Mitochondria-originated redox signalling regulates KLF-1 to promote longevity in Caenorhabditis elegans. Redox Biology, 58, 102533.

Kulczynska-Figurny, K., Bieker, J. J., & Siatecka, M. (2020). Severe anemia caused by dominant mutations in Krüppel-like factor 1 (KLF1). Mutation Research/Reviews in Mutation Research, 786, 108336.

Kulczynska, K., Bieker, J. J., & Siatecka, M. (2019). A Krüppel-like factor 1 (KLF1) mutation associated with severe congenital dyserythropoietic anemia alters its DNA-binding specificity. Molecular and cellular biology.

Herholz, M., Cepeda, E., Baumann, L., Kukat, A., Hermeling, J., Maciej, S., ... & Trifunovic, A. (2019). KLF-1 orchestrates a xenobiotic detoxification program essential for longevity of mitochondrial mutants. Nature Communications, 10(1), 3323.

Teruya, S., Okamura, T., Komai, T., Inoue, M., Iwasaki, Y., Sumitomo, S., ... & Fujio, K. (2018). Egr2-independent, Klf1-mediated induction of PD-L1 in CD4+ T cells. Scientific Reports, 8(1), 7021.

Gillinder, K. R., Magor, G., Bell, C., Ilsley, M. D., Huang, S., & Perkins, A. (2018). KLF1 acts as a pioneer transcription factor to open chromatin and facilitate recruitment of GATA1. Blood, 132, 501.

Keller, J., Vege, S., Horn, T., Keller, M. A., Leger, R. M., Aeschlimann, J., ... & Westhoff, C. M. (2018). Novel mutations in KLF1 encoding the In (Lu) phenotype reflect a diversity of clinical presentations. Transfusion, 58(1), 196-199.

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For research use only. Not intended for any clinical use.

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