Rabbit Anti-KLK3 Recombinant Antibody (
7D4) (V2LY-0725-LY1227)

Name: Rabbit Anti-KLK3 Recombinant Antibody (7D4)
SKU: V2LY-0725-LY1227
Rating: 5 (1 reviews)

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Tested Data

Rabbit Anti-KLK3 Recombinant Antibody (7D4) (V2LY-0725-LY1227) in IHC

IHC image of V2LY-0725-LY1227 diluted at 1:100 and staining in paraffin-embedded human prostate tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.

Datasheet

SDS

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Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Rabbit

Clone

7D4

Application

ELISA, IHC

Immunogen

A synthesized peptide derived from human PSA/KLK3.

Host Species

Rabbit

Specificity

Human

Antibody Isotype

IgG

Clonality

Monoclonal Antibody

Application Notes

Application	Note
IHC	1:50-1:200

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, glycerol

Preservative

Sodium azide

Concentration

Batch dependent

Purity

> 95% Purity determined by SDS-PAGE.

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

More Infomation

Target

Full Name

Kallikrein Related Peptidase 3

Entrez Gene ID

354

UniProt ID

P07288

Function

Hydrolyzes semenogelin-1 thus leading to the liquefaction of the seminal coagulum.

Biological Process

Cellular protein metabolic processTAS:Reactome
Negative regulation of angiogenesis1 PublicationNAS:UniProtKB
Positive regulation of antibacterial peptide productionManual Assertion Based On ExperimentIDA:UniProtKB
ProteolysisManual Assertion Based On ExperimentIMP:UniProtKB
Regulation of systemic arterial blood pressureManual Assertion Based On ExperimentIBA:GO_Central
Zymogen activationManual Assertion Based On ExperimentIBA:GO_Central

Cellular Location

Secreted

Raimondo, S., Gentile, M., Esposito, G., Gentile, T., Ferrara, I., Crescenzo, C., ... & Montano, L. (2021). Could kallikrein-related serine peptidase 3 be an early biomarker of environmental exposure in young women?. International Journal of Environmental Research and Public Health, 18(16), 8833.

Koistinen, H., Künnapuu, J., & Jeltsch, M. (2021). KLK3 in the Regulation of Angiogenesis—Tumorigenic or Not?. International Journal of Molecular Sciences, 22(24), 13545.

Motamedi, R. K., Sarhangi, N., Afshari, M., Sattari, M., Jamaldini, S. H., Samzadeh, M., ... & Hasanzad, M. (2019). Kallikarein‐related peptidase 3 common genetic variant and the risk of prostate cancer. Journal of Cellular Biochemistry, 120(9), 14822-14830.

Ding, W. H., Ren, K. W., Yue, C., Zou, J. G., Zuo, L., Zhang, L. F., ... & Mi, Y. Y. (2018). Association between three genetic variants in kallikrein 3 and prostate cancer risk. Bioscience reports, 38(6), BSR20181151.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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