Mouse Anti-KNTC1 Recombinant Antibody (10D9) (CBMAB-K1460-LY)

Name: Mouse Anti-KNTC1 Recombinant Antibody (10D9)
SKU: CBMAB-K1460-LY
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

10D9

Application

ELISA, WB

Immunogen

KNTC1 (NP_055523, 2100aa-2209aa) partial recombinant protein with GST tag. MW of the GST tag alone is 26kD

Specificity

Human

Antibody Isotype

IgG

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Purity

> 95% Purity determined by SDS-PAGE.

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

More Infomation

Target

Full Name

kinetochore associated 1

Introduction

This gene encodes a protein that is one of many involved in mechanisms to ensure proper chromosome segregation during cell division. Experimental evidence indicated that the encoded protein functioned in a similar manner to that of the Drosophila rough deal protein. [provided by RefSeq, Jul 2008]

Entrez Gene ID

9735

UniProt ID

P50748

Alternative Names

Kinetochore Associated 1; ROD; Rough Deal Homolog; Centromere/Kinetochore Protein; Kinetochore-Associated Protein 1; Rough Deal Homolog (Drosophila); Rough Deal Homolog; KIAA0166; HsROD; HRod;

Function

Essential component of the mitotic checkpoint, which prevents cells from prematurely exiting mitosis. Required for the assembly of the dynein-dynactin and MAD1-MAD2 complexes onto kinetochores (PubMed:11146660, PubMed:11590237, PubMed:15824131).
Its function related to the spindle assembly machinery is proposed to depend on its association in the mitotic RZZ complex.

Biological Process

Cell divisionIEA:UniProtKB-KW
Mitotic sister chromatid segregationManual Assertion Based On ExperimentIBA:GO_Central
Mitotic spindle assembly checkpoint signalingManual Assertion Based On ExperimentIDA:UniProtKB
Protein localization to kinetochore involved in kinetochore assemblyManual Assertion Based On ExperimentIBA:GO_Central
Protein-containing complex assembly1 PublicationNAS:UniProtKB
Regulation of exit from mitosis1 PublicationNAS:UniProtKB

Cellular Location

Cytoplasm
Nucleus
Chromosome, centromere, kinetochore
Cytoplasm, cytoskeleton, spindle
Dynamic pattern of localization during the cell cycle. At interphase, uniformly distributed throughout the cytoplasm and nucleus. By prophase and until late stages of prometaphase, a fraction of the total pool is concentrated at kinetochores. By metaphase, detected at kinetochores, along spindle fibers and most prominently at the poles. By late anaphase until the end of telophase, no longer detectable on kinetochores or along spindle fibers, but still present at the spindle poles.

Liu, L., Chen, H., Chen, X., Yao, C., Shen, W., & Jia, C. (2023). KNTC1 as a putative tumor oncogene in pancreatic cancer. Journal of Cancer Research and Clinical Oncology, 149(7), 3023-3031.

Yang, F., & Yuan, C. (2023). KNTC1 knockdown inhibits proliferation and metastases of liver cancer. 3 Biotech, 13(9), 309.

Wang, J., Ding, Z., Shu, W., & Zhuge, Y. (2023). The role of KNTC1 in the regulation of proliferation, migration and tumorigenesis in colorectal cancer. Cellular Signalling, 108, 110728.

Tong, H., Liu, X., Peng, C., Shen, B., & Zhu, Z. (2023). Silencing of KNTC1 inhibits hepatocellular carcinoma cells progression via suppressing PI3K/Akt pathway. Cellular Signalling, 101, 110498.

Pan, W., Wang, S., Liu, X., Wang, M., Han, X., Tian, X., ... & Wang, X. (2023). KNTC1, regulated by HPV E7, inhibits cervical carcinogenesis partially through Smad2. Experimental Cell Research, 423(1), 113458.

Zhang, W., Long, J., Tang, P., Chen, K., Guo, G., Yu, Z., ... & Xu, Z. (2023). SYT7 regulates the progression of chronic lymphocytic leukemia through interacting and regulating KNTC1. Biomarker Research, 11(1), 58.

Liu, R., Liu, R., Guo, Z., Ren, J., Huang, J., Luo, Q., & Tan, Q. (2022). shRNA‑mediated knockdown of KNTC1 inhibits non-small-cell lung cancer through regulating PSMB8. Cell Death & Disease, 13(8), 685.

Zhengxiang, Z., Yunxiang, T., Zhiping, L., & Zhimin, Y. (2021). KNTC1 knockdown suppresses cell proliferation of colon cancer. 3 Biotech, 11(6), 262.

Huang, H., Fan, X., Qiao, Y., Yang, M., & Ji, Z. (2021). Knockdown of KNTC1 inhibits the proliferation, migration and tumorigenesis of human bladder cancer cells and induces apoptosis. Critical Reviews™ in Eukaryotic Gene Expression, 31(1).

Liu, C. T., Min, L., Wang, Y. J., Li, P., Wu, Y. D., & Zhang, S. T. (2019). shRNA‑mediated knockdown of KNTC1 suppresses cell viability and induces apoptosis in esophageal squamous cell carcinoma. International Journal of Oncology, 54(3), 1053-1060.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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For research use only. Not intended for any clinical use.

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