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Mouse Anti-KNTC1 Recombinant Antibody (10D9) (CBMAB-K1460-LY)

This product is antibody recognizes KNTC1. The antibody 10D9 immunoassay techniques such as: ELISA, WB.
See all KNTC1 antibodies
Published Data
Fig.1 hRod and hZw10 form a complex in vivo.
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Fig.2 Immunofluorescence staining of HeLa cells with anti-hRod antibodies during early prometaphase.
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Fig.3 Representative images of prophase and metaphase figures from WT and rodZ3-derived syncytial embryos stained.
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Fig.4 Levels of Rod, Zw10 and Zwilch are similar in extracts of WT and rodZ3-derived syncytial embryos.
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Summary

Host Animal
Mouse
Specificity
Human
Clone
10D9
Antibody Isotype
IgG
Application
ELISA, WB

Basic Information

Immunogen
KNTC1 (NP_055523, 2100aa-2209aa) partial recombinant protein with GST tag. MW of the GST tag alone is 26kD
Specificity
Human
Antibody Isotype
IgG
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Purity
> 95% Purity determined by SDS-PAGE.
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name
kinetochore associated 1
Introduction
This gene encodes a protein that is one of many involved in mechanisms to ensure proper chromosome segregation during cell division. Experimental evidence indicated that the encoded protein functioned in a similar manner to that of the Drosophila rough deal protein. [provided by RefSeq, Jul 2008]
Entrez Gene ID
9735
UniProt ID
P50748
Alternative Names
Kinetochore Associated 1; ROD; Rough Deal Homolog; Centromere/Kinetochore Protein; Kinetochore-Associated Protein 1; Rough Deal Homolog (Drosophila); Rough Deal Homolog; KIAA0166; HsROD; HRod;
Function
Essential component of the mitotic checkpoint, which prevents cells from prematurely exiting mitosis. Required for the assembly of the dynein-dynactin and MAD1-MAD2 complexes onto kinetochores (PubMed:11146660, PubMed:11590237, PubMed:15824131).
Its function related to the spindle assembly machinery is proposed to depend on its association in the mitotic RZZ complex.
Biological Process
Cell divisionIEA:UniProtKB-KW
Mitotic sister chromatid segregationManual Assertion Based On ExperimentIBA:GO_Central
Mitotic spindle assembly checkpoint signalingManual Assertion Based On ExperimentIDA:UniProtKB
Protein localization to kinetochore involved in kinetochore assemblyManual Assertion Based On ExperimentIBA:GO_Central
Protein-containing complex assembly1 PublicationNAS:UniProtKB
Regulation of exit from mitosis1 PublicationNAS:UniProtKB
Cellular Location
Cytoplasm
Nucleus
Chromosome, centromere, kinetochore
Cytoplasm, cytoskeleton, spindle
Dynamic pattern of localization during the cell cycle. At interphase, uniformly distributed throughout the cytoplasm and nucleus. By prophase and until late stages of prometaphase, a fraction of the total pool is concentrated at kinetochores. By metaphase, detected at kinetochores, along spindle fibers and most prominently at the poles. By late anaphase until the end of telophase, no longer detectable on kinetochores or along spindle fibers, but still present at the spindle poles.

Liu, L., Chen, H., Chen, X., Yao, C., Shen, W., & Jia, C. (2023). KNTC1 as a putative tumor oncogene in pancreatic cancer. Journal of Cancer Research and Clinical Oncology, 149(7), 3023-3031.

Yang, F., & Yuan, C. (2023). KNTC1 knockdown inhibits proliferation and metastases of liver cancer. 3 Biotech, 13(9), 309.

Wang, J., Ding, Z., Shu, W., & Zhuge, Y. (2023). The role of KNTC1 in the regulation of proliferation, migration and tumorigenesis in colorectal cancer. Cellular Signalling, 108, 110728.

Tong, H., Liu, X., Peng, C., Shen, B., & Zhu, Z. (2023). Silencing of KNTC1 inhibits hepatocellular carcinoma cells progression via suppressing PI3K/Akt pathway. Cellular Signalling, 101, 110498.

Pan, W., Wang, S., Liu, X., Wang, M., Han, X., Tian, X., ... & Wang, X. (2023). KNTC1, regulated by HPV E7, inhibits cervical carcinogenesis partially through Smad2. Experimental Cell Research, 423(1), 113458.

Zhang, W., Long, J., Tang, P., Chen, K., Guo, G., Yu, Z., ... & Xu, Z. (2023). SYT7 regulates the progression of chronic lymphocytic leukemia through interacting and regulating KNTC1. Biomarker Research, 11(1), 58.

Liu, R., Liu, R., Guo, Z., Ren, J., Huang, J., Luo, Q., & Tan, Q. (2022). shRNA‑mediated knockdown of KNTC1 inhibits non-small-cell lung cancer through regulating PSMB8. Cell Death & Disease, 13(8), 685.

Zhengxiang, Z., Yunxiang, T., Zhiping, L., & Zhimin, Y. (2021). KNTC1 knockdown suppresses cell proliferation of colon cancer. 3 Biotech, 11(6), 262.

Huang, H., Fan, X., Qiao, Y., Yang, M., & Ji, Z. (2021). Knockdown of KNTC1 inhibits the proliferation, migration and tumorigenesis of human bladder cancer cells and induces apoptosis. Critical Reviews™ in Eukaryotic Gene Expression, 31(1).

Liu, C. T., Min, L., Wang, Y. J., Li, P., Wu, Y. D., & Zhang, S. T. (2019). shRNA‑mediated knockdown of KNTC1 suppresses cell viability and induces apoptosis in esophageal squamous cell carcinoma. International Journal of Oncology, 54(3), 1053-1060.

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For research use only. Not intended for any clinical use.

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