Rabbit Anti-KRT12 Recombinant Antibody (BA0169) (CBMAB-0438CQ)

Name: Rabbit Anti-KRT12 Recombinant Antibody (BA0169)
SKU: CBMAB-0438CQ
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Rabbit

Clone

BA0169

Application

IF, IHC-P, WB

Immunogen

Human Keratin 12 aa 250 to the C-terminus

Specificity

Human, Mouse, Rat

Antibody Isotype

IgG

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Purity

>95% as determined by analysis by SDS-PAGE

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

More Infomation

Target

Full Name

Keratin 12

Introduction

KRT12 encodes the type I intermediate filament chain keratin 12, expressed in corneal epithelia. Mutations in this gene lead to Meesmann corneal dystrophy. Kao et al. (1996) stated that the corneal-specific nature of keratin-12 gene expression signifies keratin-12 plays a unique role in maintaining normal corneal epithelial function. Nishida et al. (1997) presented the exon-intron boundary structure of the KRT12 gene. The gene contains 8 exons and spans approximately 6 kb of genomic DNA.

Entrez Gene ID

Human	3859
Mouse	268482
Rat	360625

UniProt ID

Human	Q99456
Mouse	Q64291
Rat	Q6IFW5

Alternative Names

K12

Function

Involved in corneal epithelium organization, integrity and corneal keratin expression.

Biological Process

Cornea development in camera-type eyeManual Assertion Based On ExperimentIMP:UniProtKB
Morphogenesis of an epitheliumManual Assertion Based On ExperimentIMP:UniProtKB
Visual perceptionManual Assertion Based On ExperimentTAS:ProtInc

Cellular Location

Cytosol
Extracellular exosome
Intermediate filament

Involvement in disease

Corneal dystrophy, Meesmann 1 (MECD1):
A form of Meesmann corneal dystrophy, a corneal disease characterized by fragility of the anterior corneal epithelium. Histological examination shows a disorganized and thickened epithelium with widespread cytoplasmic vacuolation and numerous small, round, debris-laden intraepithelial cysts. Patients are usually asymptomatic until adulthood when rupture of the corneal microcysts may cause erosions, producing clinical symptoms such as photophobia, contact lens intolerance and intermittent diminution of visual acuity. Rarely, subepithelial scarring causes irregular corneal astigmatism and permanent visual impairment. MECD1 inheritance is autosomal dominant.

Shi, L., Stachon, T., Käsmann-Kellner, B., Seitz, B., Szentmáry, N., & Latta, L. (2020). Keratin 12 mRNA expression could serve as an early corneal marker for limbal explant cultures. Cytotechnology, 72(2), 239-245.

Latta, L., Viestenz, A., Stachon, T., Colanesi, S., Szentmáry, N., Seitz, B., & Käsmann-Kellner, B. (2018). Human aniridia limbal epithelial cells lack expression of keratins K3 and K12. Experimental Eye Research, 167, 100-109.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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Rabbit Anti-KRT12 Recombinant Antibody (BA0170) (CAT#: CBMAB-0439CQ)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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