Mouse Anti-KRT18 Recombinant Antibody (M30) (CBMAB-C4007-CQ)

Name: Mouse Anti-KRT18 Recombinant Antibody (M30)
SKU: CBMAB-C4007-CQ
Rating: 5 (1 reviews)

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Datasheet

SDS

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Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

M30

Application

WB, ICC, FC, IHC

Immunogen

Keratin 18 (K18) fragments purified from supernatant from human carcinoma cell line WiDr CCL218

Specificity

Human

Antibody Isotype

IgG2b

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

More Infomation

Target

Full Name

Keratin 18

Introduction

KRT18 encodes the type I intermediate filament chain keratin 18. Keratin 18, together with its filament partner keratin 8, are perhaps the most commonly found members of the intermediate filament gene family. They are expressed in single layer epithelial tissues of the body. Mutations in this gene have been linked to cryptogenic cirrhosis. Two transcript variants encoding the same protein have been found for this gene.

Entrez Gene ID

3875

UniProt ID

P05783

Alternative Names

Keratin 18; Cell Proliferation-Inducing Gene 46 Protein; Keratin 18, Type I; CK-18; CYK18; K18;

Function

Involved in the uptake of thrombin-antithrombin complexes by hepatic cells (By similarity).
When phosphorylated, plays a role in filament reorganization. Involved in the delivery of mutated CFTR to the plasma membrane. Together with KRT8, is involved in interleukin-6 (IL-6)-mediated barrier protection.

Biological Process

Anatomical structure morphogenesisManual Assertion Based On ExperimentTAS:ProtInc
Cell cycleIEA:UniProtKB-KW
Extrinsic apoptotic signaling pathwayIEA:Ensembl
Golgi to plasma membrane CFTR protein transportManual Assertion Based On ExperimentIDA:UniProtKB
Hepatocyte apoptotic processIEA:Ensembl
Intermediate filament cytoskeleton organizationManual Assertion Based On ExperimentIDA:UniProtKB
Negative regulation of apoptotic processManual Assertion Based On ExperimentIDA:UniProtKB
Tumor necrosis factor-mediated signaling pathwayIEA:Ensembl

Cellular Location

Cytoplasm, perinuclear region
Nucleus, nucleolus

Involvement in disease

Cirrhosis (CIRRH):
A liver disease characterized by severe panlobular liver-cell swelling with Mallory body formation, prominent pericellular fibrosis, and marked deposits of copper. Clinical features include abdomen swelling, jaundice and pulmonary hypertension.

PTM

Phosphorylation at Ser-34 increases during mitosis. Hyperphosphorylated at Ser-53 in diseased cirrhosis liver. Phosphorylation increases by IL-6.
Proteolytically cleaved by caspases during epithelial cell apoptosis. Cleavage occurs at Asp-238 by either caspase-3, caspase-6 or caspase-7.
O-GlcNAcylation increases solubility, and decreases stability by inducing proteasomal degradation.

Cui, J., Ma, Q., Zhang, C., Li, Y., Liu, J., Xie, K., ... & Tang, C. (2023). Keratin 18 Depletion as a Possible Mechanism for the Induction of Apoptosis and Ferroptosis in the Rat Hippocampus After Hypobaric Hypoxia. Neuroscience, 513, 64-75.

Wang, P. B., Chen, Y., Ding, G. R., Du, H. W., & Fan, H. Y. (2021). Keratin 18 induces proliferation, migration, and invasion in gastric cancer via the MAPK signalling pathway. Clinical and Experimental Pharmacology and Physiology, 48(1), 147-156.

Yin, S., Song, M., Zhao, R., Liu, X., Kang, W. K., Lee, J. M., ... & Lee, M. H. (2020). Xanthohumol inhibits the growth of keratin 18-overexpressed esophageal squamous cell carcinoma in vitro and in vivo. Frontiers in cell and developmental biology, 8, 366.

Muriel, J. M., O’Neill, A., Kerr, J. P., Kleinhans-Welte, E., Lovering, R. M., & Bloch, R. J. (2020). Keratin 18 is an integral part of the intermediate filament network in murine skeletal muscle. American Journal of Physiology-Cell Physiology, 318(1), C215-C224.

Sirniö, P., Väyrynen, J. P., Mutt, S. J., Herzig, K. H., Walkowiak, J., Klintrup, K., ... & Tuomisto, A. (2020). Systemic inflammation is associated with circulating cell death released keratin 18 fragments in colorectal cancer. Oncoimmunology, 9(1), 1783046.

Golob-Schwarzl, N., Bettermann, K., Mehta, A. K., Kessler, S. M., Unterluggauer, J., Krassnig, S., ... & Haybaeck, J. (2019). High keratin 8/18 ratio predicts aggressive hepatocellular cancer phenotype. Translational oncology, 12(2), 256-268.

Zhang, J., Hu, S., & Li, Y. (2019). KRT18 is correlated with the malignant status and acts as an oncogene in colorectal cancer. Bioscience reports, 39(8), BSR20190884.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

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