Mouse Anti-KRT20 Recombinant Antibody (CBT3089) (V2LY-0625-LY1337)

Name: Mouse Anti-KRT20 Recombinant Antibody (CBT3089)
SKU: V2LY-0625-LY1337
Rating: 5 (1 reviews)

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Tested Data

Mouse Anti-KRT20 Recombinant Antibody (CBT3089) (V2LY-0625-LY1337) in ELISA

Mouse Anti-KRT20 Recombinant Antibody (CBT3089) (V2LY-0625-LY1337) in WB

Mouse Anti-KRT20 Recombinant Antibody (CBT3089) (V2LY-0625-LY1337) in IHC

Datasheet

SDS

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Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

CBT3089

Application

IHC

Immunogen

Purified recombinant fragment of human KRT20 (AA: 1-150) expressed in E. Coli.

Host Species

Mouse

Specificity

Human

Antibody Isotype

IgG1

Clonality

Monoclonal Antibody

Application Notes

Application	Note
WB	1:500-1:2,000
IHC-P	1:200-1:1,000
ELISA	1:10,000

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS

Preservative

Sodium azide

Concentration

Batch dependent

Purity

> 95% Purity determined by SDS-PAGE.

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

More Infomation

Target

Full Name

Keratin 20

Entrez Gene ID

54474

UniProt ID

P35900

Function

Plays a significant role in maintaining keratin filament organization in intestinal epithelia. When phosphorylated, plays a role in the secretion of mucin in the small intestine (By similarity).

Biological Process

Apoptotic processIEA:UniProtKB-KW
Cellular response to starvationIEA:Ensembl
Intermediate filament organizationManual Assertion Based On ExperimentIMP:UniProtKB
Regulation of protein secretionIEA:Ensembl

Cellular Location

Cytoplasm

PTM

Hyperphosphorylation at Ser-13 ^oCcurs during the early stages of apoptosis but becomes less prominent during the later stages. Phosphorylation at Ser-13 also increases in response to stress brought on by cell injury (By similarity).
Proteolytically cleaved by caspases during apoptosis. Cleavage occurs at Asp-228.

Lupsa, N., Szegedi, Á., Gézsi, A., Vuncs, Z., Masszi, T., Mikala, G., ... & Pós, Z. (2022). Decreased plasma level of cytokeratin 20 (KRT20) is indicative of the emergence and severity of acute GvHD irrespective to the type of organ involvement. Biomedicines, 10(3), 519.

Jütte, H., Reike, M., Wirtz, R. M., Kriegmair, M., Erben, P., Tully, K., ... & Roghmann, F. (2021). Krt20, krt5, esr1 and erbb2 expression can predict pathologic outcome in patients undergoing neoadjuvant chemotherapy and radical cystectomy for muscle-invasive bladder cancer. Journal of personalized medicine, 11(6), 473.

Hung, C. S., Wang, Y. C., Guo, J. W., Yang, R. N., Lee, C. L., Shen, M. H., ... & Liu, C. Y. (2020). Expression pattern of placenta specific 8 and keratin 20 in different types of gastrointestinal cancer. Molecular Medicine Reports, 21(2), 659-666.

Eckstein, M., Wirtz, R. M., Gross-Weege, M., Breyer, J., Otto, W., Stoehr, R., ... & Erben, P. (2018). mRNA-expression of KRT5 and KRT20 defines distinct prognostic subgroups of muscle-invasive urothelial bladder cancer correlating with histological variants. International journal of molecular sciences, 19(11), 3396.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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