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Rat Anti-Lag3 Recombinant Antibody (CBYJL-2434) (CBMAB-L2771-YJ)

Provided herein is a Rat monoclonal antibody, which binds to Lymphocyte-Activation Gene 3 (Lag3). The antibody can be used for immunoassay techniques, such as FC.
See all Lag3 antibodies

Summary

Host Animal
Rat
Specificity
Mouse
Clone
CBYJL-2434
Antibody Isotype
IgG2b
Application
FC

Basic Information

Specificity
Mouse
Antibody Isotype
IgG2b
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Buffer
PBS, 5% trehalose
Storage
Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Epitope
aa 23-442

Target

Full Name
lymphocyte activating 3
Introduction
Lag3 is involved in lymphocyte activation. Lag3 binds to HLA class-II antigens (By similarity).
Entrez Gene ID
UniProt ID
Alternative Names
Ly66; CD223; LAG-3; lymphocyte activation gene 3 protein
Function
Lymphocyte activation gene 3 protein: Inhibitory receptor on antigen activated T-cells (PubMed:7805750, PubMed:8647185, PubMed:20421648).
Delivers inhibitory signals upon binding to ligands, such as FGL1 (By similarity).
FGL1 constitutes a major ligand of LAG3 and is responsible for LAG3 T-cell inhibitory function (By similarity).
Following TCR engagement, LAG3 associates with CD3-TCR in the immunological synapse and directly inhibits T-cell activation (By similarity).
May inhibit antigen-specific T-cell activation in synergy with PDCD1/PD-1, possibly by acting as a coreceptor for PDCD1/PD-1 (By similarity).
Negatively regulates the proliferation, activation, effector function and homeostasis of both CD8(+) and CD4(+) T-cells (PubMed:7805750, PubMed:8647185, PubMed:20421648).
Also mediates immune tolerance: constitutively expressed on a subset of regulatory T-cells (Tregs) and contributes to their suppressive function (By similarity).
Also acts as a negative regulator of plasmacytoid dendritic cell (pDCs) activation (By similarity).
Binds MHC class II (MHC-II); the precise role of MHC-II-binding is however unclear (PubMed:8647185).
Secreted lymphocyte activation gene 3 protein
May function as a ligand for MHC class II (MHC-II) on antigen-presenting cells (APC), promoting APC activation/maturation and driving Th1 immune response.
Biological Process
Adaptive immune responseIEA:UniProtKB-KW
Cell surface receptor signaling pathwayISS:UniProtKB
Negative regulation of interleukin-2 productionIEA:Ensembl
Negative regulation of regulatory T cell differentiationISS:UniProtKB
Plasmacytoid dendritic cell activationISS:UniProtKB
Positive regulation of natural killer cell mediated cytotoxicityIEA:Ensembl
Regulation of immune responseISS:UniProtKB
Cellular Location
Lymphocyte activation gene 3 protein:
Cell membrane
Secreted lymphocyte activation gene 3 protein:
Secreted
Produced following cleavage of the main chain.
Topology
Extracellular: 23-450
Helical: 451-471
Cytoplasmic: 472-525
PTM
Lymphocyte activation gene 3 protein
Proteolytically cleaved by ADAM10 and ADAM17 within the connecting peptide region, leading to release of Secreted lymphocyte activation gene 3 protein (sLAG-3). ADAM10 mediates constitutive cleavage, but cleavage increases following T-cell activation, whereas shedding by ADAM17 is induced by TCR signaling in a PRKCQ-dependent manner.

Zhao, L., Wang, H., Xu, K., Liu, X., & He, Y. (2022). Update on lymphocyte-activation gene 3 (LAG-3) in cancers: From biological properties to clinical applications. Chinese Medical Journal, 135(10), 1203-1212.

Lythgoe, M. P., Liu, D. S. K., Annels, N. E., Krell, J., & Frampton, A. E. (2021). Gene of the month: Lymphocyte-activation gene 3 (LAG-3). Journal of Clinical Pathology, 74(9), 543-547.

Slevin, S. M., Garner, L. C., Lahiff, C., Tan, M., Wang, L. M., Ferry, H., ... & Keshav, S. (2020). Lymphocyte activation gene (LAG)-3 is associated with mucosal inflammation and disease activity in ulcerative colitis. Journal of Crohn's and Colitis, 14(10), 1446-1461.

Gebauer, F., Krämer, M., Bruns, C., Schlößer, H. A., Thelen, M., Lohneis, P., ... & Quaas, A. (2020). Lymphocyte activation gene-3 (LAG3) mRNA and protein expression on tumour infiltrating lymphocytes (TILs) in oesophageal adenocarcinoma. Journal of cancer research and clinical oncology, 146, 2319-2327.

Atkinson, V., Khattak, A., Haydon, A., Eastgate, M., Roy, A., Prithviraj, P., ... & Triebel, F. (2020). Eftilagimod alpha, a soluble lymphocyte activation gene-3 (LAG-3) protein plus pembrolizumab in patients with metastatic melanoma. Journal for immunotherapy of cancer, 8(2).

Yu, X., Huang, X., Chen, X., Liu, J., Wu, C., Pu, Q., ... & Zhou, L. (2019, August). Characterization of a novel anti-human lymphocyte activation gene 3 (LAG-3) antibody for cancer immunotherapy. In MAbs (Vol. 11, No. 6, pp. 1139-1148). Taylor & Francis.

Ruffo, E., Wu, R. C., Bruno, T. C., Workman, C. J., & Vignali, D. A. (2019, April). Lymphocyte-activation gene 3 (LAG3): The next immune checkpoint receptor. In Seminars in immunology (Vol. 42, p. 101305). Academic Press.

Saleh, R. R., Peinado, P., Fuentes-Antrás, J., Pérez-Segura, P., Pandiella, A., Amir, E., & Ocaña, A. (2019). Prognostic value of lymphocyte-activation gene 3 (LAG3) in cancer: a meta-analysis. Frontiers in oncology, 9, 1040.

Zhang, Y., Liu, Y. D., Luo, Y. L., Liu, B. L., Huang, Q. T., Wang, F., & Zhong, Q. (2018). Prognostic value of lymphocyte activation gene-3 (LAG-3) expression in esophageal squamous cell carcinoma. Journal of Cancer, 9(22), 4287.

Wang, Y., Dong, T., Xuan, Q., Zhao, H., Qin, L., & Zhang, Q. (2018). Lymphocyte-activation gene-3 expression and prognostic value in neoadjuvant-treated triple-negative breast cancer. Journal of breast cancer, 21(2), 124-133.

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For research use only. Not intended for any clinical use.

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