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Rat Anti-Lamp1 Recombinant Antibody (CBYJL-2520) (CBMAB-L2926-YJ)

Provided herein is a Rat monoclonal antibody, which binds to Lysosomal-Associated Membrane Protein 1 (Lamp1). The antibody can be used for immunoassay techniques, such as FC, IHC, IP, WB.
See all Lamp1 antibodies

Summary

Host Animal
Rat
Specificity
Mouse
Clone
CBYJL-2520
Antibody Isotype
IgG2a, κ
Application
FC, IHC, IP, WB

Basic Information

Immunogen
Plasma membrane fraction of mouse embryo NIH3T3 cell line.
Specificity
Mouse
Antibody Isotype
IgG2a, κ
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Buffer
PBS, pH 7.4
Preservative
Sodium Azide
Storage
Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
LAMP1
Introduction
Lysosomal-associated membrane protein 1 presents carbohydrate ligands to selectins. Lysosomal-associated membrane protein 1 is also implicated in tumor cell metastasis.
Entrez Gene ID
UniProt ID
Alternative Names
P2B; Perk; LGP-A; CD107a; Lamp-1; LGP-120; lysosome-associated membrane glycoprotein 1; 120 kDa lysosomal membrane glycoprotein; CD107 antigen-like family member A; LYSOSOME-ASSOCIATED MEMBRANE GLYCOPROTEIN 1 PRECURSOR (LAMP-1) (LGP-A) (LGP-120) (CD107A) (P2B); Lamp I; lysosomal membrane glycoprotein 1; lysosomal membrane glycoprotein A; lysosome-associated membrane protein 1
Function
Lysosomal membrane glycoprotein which plays an important role in lysosome biogenesis, autophagy, and cholesterol homeostasis (By similarity).
Plays also an important role in NK-cells cytotoxicity (PubMed:23632890, PubMed:2022921).
Mechanistically, participates in cytotoxic granule movement to the cell surface and perforin trafficking to the lytic granule (PubMed:23632890).
In addition, protects NK-cells from degranulation-associated damage induced by their own cytotoxic granule content (PubMed:23847195).
Presents carbohydrate ligands to selectins. Also implicated in tumor cell metastasis.
(Microbial infection) Acts as a receptor for Lassa virus glycoprotein (PubMed:24970085).
Promotes also fusion of the virus with host membrane in less acidic endosomes (PubMed:29295909).
(Microbial infection) Supports the FURIN-mediated cleavage of mumps virus fusion protein F by interacting with both FURIN and the unprocessed form but not the processed form of the viral protein F.
Biological Process
Establishment of protein localization to organelleManual Assertion Based On ExperimentIMP:UniProtKB
Golgi to lysosome transportManual Assertion Based On ExperimentIMP:UniProtKB
Granzyme-mediated programmed cell death signaling pathwayManual Assertion Based On ExperimentIMP:GO_Central
Positive regulation of natural killer cell degranulationManual Assertion Based On ExperimentIMP:UniProtKB
Positive regulation of natural killer cell mediated cytotoxicityManual Assertion Based On ExperimentIMP:UniProtKB
Protein stabilizationISS:CAFA
Regulation of organelle transport along microtubuleManual Assertion Based On ExperimentIMP:UniProtKB
Cellular Location
Cell membrane
Endosome membrane
Lysosome membrane
Late endosome membrane
Cytolytic granule membrane
This protein shuttles between lysosomes, endosomes, and the plasma membrane (By similarity).
Colocalizes with OSBPL1A at the late endosome (PubMed:16176980).
Topology
Lumenal: 29-382
Helical: 383-405
Cytoplasmic: 406-417
PTM
O- and N-glycosylated; some of the 18 N-linked glycans are polylactosaminoglycans. The glycosylation of N-76 is essential for Lassa virus entry into cells.

Chaudhry, N., Sica, M., Surabhi, S., Hernandez, D. S., Mesquita, A., Selimovic, A., ... & Jenny, A. (2022). Lamp1 mediates lipid transport, but is dispensable for autophagy in Drosophila. Autophagy, 18(10), 2443-2458.

Nassal, J. P., Murphy, F. H., Toonen, R. F., & Verhage, M. (2022). Differential axonal trafficking of Neuropeptide Y-, LAMP1-, and RAB7-tagged organelles in vivo. Elife, 11, e81721.

Baba, K., Kuwada, S., Nakao, A., Li, X., Okuda, N., Nishida, A., ... & Kataoka, T. (2020). Different localization of lysosomal-associated membrane protein 1 (LAMP1) in mammalian cultured cell lines. Histochemistry and cell biology, 153, 199-213.

Chen, H., Li, L., Hu, J., Zhao, Z., Ji, L., Cheng, C., ... & Sun, B. (2019). UBL4A inhibits autophagy-mediated proliferation and metastasis of pancreatic ductal adenocarcinoma via targeting LAMP1. Journal of Experimental & Clinical Cancer Research, 38, 1-18.

Deng, L., Liu, G., Zheng, C., Zhang, L., Kang, Y., & Yang, F. (2019). Circ-LAMP1 promotes T-cell lymphoblastic lymphoma progression via acting as a ceRNA for miR-615-5p to regulate DDR2 expression. Gene, 701, 146-151.

Wang, X., Li, C., Wang, Q., Li, W., Guo, D., Zhang, X., ... & Wang, Y. (2019). Tanshinone IIA restores dynamic balance of autophagosome/autolysosome in doxorubicin-induced cardiotoxicity via targeting beclin1/LAMP1. Cancers, 11(7), 910.

Hulseberg, C. E., Fénéant, L., Szymańska, K. M., & White, J. M. (2018). Lamp1 increases the efficiency of Lassa virus infection by promoting fusion in less acidic endosomal compartments. MBio, 9(1), 10-1128.

Cheng, X. T., Xie, Y. X., Zhou, B., Huang, N., Farfel-Becker, T., & Sheng, Z. H. (2018). Characterization of LAMP1-labeled nondegradative lysosomal and endocytic compartments in neurons. Journal of Cell Biology, 217(9), 3127-3139.

Cheng, X. T., Xie, Y. X., Zhou, B., Huang, N., Farfel-Becker, T., & Sheng, Z. H. (2018). Revisiting LAMP1 as a marker for degradative autophagy-lysosomal organelles in the nervous system. Autophagy, 14(8), 1472-1474.

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For research use only. Not intended for any clinical use.

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We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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