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Mouse Anti-LASP1 Recombinant Antibody (8C6) (CBMAB-L0706-YJ)

Provided herein is a Mouse monoclonal antibody, which binds to Lim And Sh3 Protein 1 (LASP1). The antibody can be used for immunoassay techniques, such as WB, IM.
See all LASP1 antibodies
Published Data
Fig.1 Lasp1+/+ and Lasp1−/− MEFs were fixed and stained with a zyxin polyclonal antibody and Cy3-tagged donkey anti-rabbit secondary antibody to localize zyxin, a Lasp1 monoclonal antibody, and Cy5-tagged donkey anti-mouse antibody to localize Lasp1.
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Fig.2 Effects of chronic loss of Lasp1 on zyxin and LPP expression as assessed by Western blotting.
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Fig.3 Western blots of gastric mucosal extracts from the same mice showing differences in the Lasp-1 signal compared with the β-actin signal.
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Fig.4 Immunolocalization of Lasp-1 in cultured mouse osteoclasts.
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Summary

Host Animal
Mouse
Specificity
Human, Mouse, Rat, Rabbit
Clone
8C6
Antibody Isotype
IgG1
Application
WB, IM

Basic Information

Immunogen
Clone 8C6 was raised against a KLH-coupled peptide from a conserved region of LASP-1.
Specificity
Human, Mouse, Rat, Rabbit
Antibody Isotype
IgG1
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Buffer
PBS, pH 7.4
Storage
Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
LIM and SH3 protein 1
Introduction
LASP1 is a member of a subfamily of LIM proteins, characterized by a LIM motif and a domain of Src homology region 3, and also a member of the nebulin family of actin-binding proteins. LASP1 is a cAMP and cGMP dependent signaling protein and binds to the actin cytoskeleton at extensions of the cell membrane. LASP1 has been linked to metastatic breast cancer, hematopoetic tumors such as B-cell lymphomas, and colorectal cancer.
Entrez Gene ID
Human3927
Mouse16796
Rat29278
Rabbit100009568
UniProt ID
HumanQ14847
MouseQ61792
RatQ99MZ8
RabbitO77506
Alternative Names
Lasp-1; MLN50
Function
Plays an important role in the regulation of dynamic actin-based, cytoskeletal activities. Agonist-dependent changes in LASP1 phosphorylation may also serve to regulate actin-associated ion transport activities, not only in the parietal cell but also in certain other F-actin-rich secretory epithelial cell types (By similarity).
Biological Process
Ion transportISS:UniProtKB
Cellular Location
Cytoplasm, cell cortex
Cytoplasm, cytoskeleton
Associated with the F-actin rich cortical cytoskeleton.

Butt, E., Howard, C. M., & Raman, D. (2022). LASP1 in cellular signaling and gene expression: more than just a cytoskeletal regulator. Cells, 11(23), 3817.

Xue, Q., Jiang, H., Wang, J., & Wei, D. (2021). LASP1 induces epithelial-mesenchymal transition in lung cancer through the TGF-β1/Smad/Snail pathway. Canadian Respiratory Journal, 2021.

Chen, N., Han, X., Bai, X., Yin, B., & Wang, Y. (2020). LASP1 induces colorectal cancer proliferation and invasiveness through Hippo signaling and Nanog mediated EMT. American journal of translational research, 12(10), 6490.

Yin, L., Chen, Y., Zhou, Y., Deng, G., Han, Y., Guo, C., ... & Shen, H. (2019). Increased long noncoding RNA LASP1‐AS is critical for hepatocellular carcinoma tumorigenesis via upregulating LASP1. Journal of cellular physiology, 234(8), 13493-13509.

Zhang, Z., Lin, W., Gao, L., Chen, K., Yang, C., Zhuang, L., ... & Lin, J. (2019). Hsa_circ_0004370 promotes esophageal cancer progression through miR-1294/LASP1 pathway. Bioscience Reports, 39(5), BSR20182377.

Zhou, R., Shao, Z., Liu, J., Zhan, W., Gao, Q., Pan, Z., ... & Zhao, L. (2018). COPS5 and LASP1 synergistically interact to downregulate 14‐3‐3σ expression and promote colorectal cancer progression via activating PI3K/AKT pathway. International journal of cancer, 142(9), 1853-1864.

Shi, J., Guo, J., & Li, X. (2018). Role of LASP-1, a novel SOX9 transcriptional target, in the progression of lung cancer. International journal of oncology, 52(1), 179-188.

Gao, Q., Tang, L., Wu, L., Li, K., Wang, H., Li, W., ... & Zhao, L. (2018). LASP1 promotes nasopharyngeal carcinoma progression through negatively regulation of the tumor suppressor PTEN. Cell Death & Disease, 9(3), 393.

Butt, E., & Raman, D. (2018). New frontiers for the cytoskeletal protein LASP1. Frontiers in oncology, 8, 391.

Liu, Y., Gao, Y., Li, D., He, L., Lao, I. W., Hao, B., ... & Cao, Y. (2018). LASP1 promotes glioma cell proliferation and migration and is negatively regulated by miR-377-3p. Biomedicine & Pharmacotherapy, 108, 845-851.

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For research use only. Not intended for any clinical use.

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We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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