Mouse Anti-LAT Recombinant Antibody (CBYJL-1203) (CBMAB-L0712-YJ)

Name: Mouse Anti-LAT Recombinant Antibody (CBYJL-1203)
SKU: CBMAB-L0712-YJ
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

CBYJL-1203

Application

IHC-P, IHC-Fr

Specificity

Human

Antibody Isotype

IgG1

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Buffer

PBS, pH 7.4

Storage

Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

More Infomation

Target

Full Name

linker for activation of T cells

Introduction

LAT is phosphorylated by ZAP-70/Syk protein tyrosine kinases following activation of the T-cell antigen receptor (TCR) signal transduction pathway. This transmembrane protein localizes to lipid rafts and plays a role as a docking site for SH2 domain-containing proteins. Upon phosphorylation, this protein recruits multiple adaptor proteins and downstream signaling molecules into multimolecular signaling complexes located near the site of TCR engagement. Alternative splicing results in multiple transcript variants encoding different isoforms. Diseases associated with LAT include Immunodeficiency 52 and Erythrokeratodermia Variabilis Et Progressiva 2. Among its related pathways are Immune response Fc epsilon RI pathway and T cell receptor signaling pathway.

Entrez Gene ID

27040

UniProt ID

O43561

Alternative Names

IMD521; pp36; LAT

Function

Required for TCR (T-cell antigen receptor)- and pre-TCR-mediated signaling, both in mature T-cells and during their development. Involved in FCGR3 (low affinity immunoglobulin gamma Fc region receptor III)-mediated signaling in natural killer cells and FCER1 (high affinity immunoglobulin epsilon receptor)-mediated signaling in mast cells. Couples activation of these receptors and their associated kinases with distal intracellular events such as mobilization of intracellular calcium stores, PKC activation, MAPK activation or cytoskeletal reorganization through the recruitment of PLCG1, GRB2, GRAP2, and other signaling molecules.

Biological Process

Adaptive immune responseIEA:UniProtKB-KW
Calcium-mediated signalingManual Assertion Based On ExperimentIMP:HGNC-UCL
Gene expressionIEA:Ensembl
Immune responseManual Assertion Based On ExperimentIDA:HGNC-UCL
Inflammatory responseManual Assertion Based On ExperimentIBA:GO_Central
Integrin-mediated signaling pathwayManual Assertion Based On ExperimentIDA:HGNC-UCL
Intracellular signal transductionManual Assertion Based On ExperimentIDA:HGNC-UCL
Lymphocyte homeostasisIEA:Ensembl
Mast cell degranulationIEA:UniProtKB-KW
Positive regulation of protein kinase activityManual Assertion Based On ExperimentIEP:CACAO
Ras protein signal transductionManual Assertion Based On ExperimentIMP:HGNC-UCL
Regulation of T cell activationManual Assertion Based On ExperimentIMP:HGNC-UCL
T cell activationManual Assertion Based On ExperimentTAS:UniProtKB

Cellular Location

Cell membrane
Present in lipid rafts.

Involvement in disease

Immunodeficiency 52 (IMD52):
An autosomal recessive primary immunodeficiency characterized by T-cell abnormalities, resulting in severe combined immunodeficiency, autoimmune disease, progressive lymphopenia and hypogammaglobulinemia, and lymphoproliferation with splenomegaly. Patients develop severe recurrent infections from infancy.

Topology

Extracellular: 1-4
Helical: 5-27
Cytoplasmic: 28-262

PTM

Phosphorylated on tyrosines by ZAP70 upon TCR activation, or by SYK upon other immunoreceptor activation; which leads to the recruitment of multiple signaling molecules. Is one of the most prominently tyrosine-phosphorylated proteins detected following TCR engagement. May be dephosphorylated by PTPRJ. Phosphorylated by ITK leading to the recruitment of VAV1 to LAT-containing complexes.
Palmitoylation of Cys-26 and Cys-29 is required for raft targeting and efficient phosphorylation.

Lo, W. L., Kuhlmann, M., Rizzuto, G., Ekiz, H. A., Kolawole, E. M., Revelo, M. P., ... & Weiss, A. (2023). A single-amino acid substitution in the adaptor LAT accelerates TCR proofreading kinetics and alters T-cell selection, maintenance and function. Nature immunology, 24(4), 676-689.

Lo, W. L., & Weiss, A. (2021). Adapting T cell receptor ligand discrimination capability via LAT. Frontiers in immunology, 12, 673196.

Li, Y., Tunbridge, H. M., Britton, G. J., Hill, E. V., Sinai, P., Cirillo, S., ... & Wülfing, C. (2021). A LAT-Based Signaling Complex in the Immunological Synapse as Determined with Live Cell Imaging Is Less Stable in T Cells with Regulatory Capability. Cells, 10(2), 418.

Liu, C., Xu, X., Han, L., Wan, X., Zheng, L., Li, C., ... & Wang, H. (2020). LRCH1 deficiency enhances LAT signalosome formation and CD8+ T cell responses against tumors and pathogens. Proceedings of the National Academy of Sciences, 117(32), 19388-19398.

Lo, W. L., Shah, N. H., Rubin, S. A., Zhang, W., Horkova, V., Fallahee, I. R., ... & Weiss, A. (2019). Slow phosphorylation of a tyrosine residue in LAT optimizes T cell ligand discrimination. Nature immunology, 20(11), 1481-1493.

Fan, D., Li, J., Li, Y., Guo, Y., Zhang, X., Wang, W., ... & Ji, Z. (2019). Protein 4.1 R negatively regulates CD8+ T‐cell activation by modulating phosphorylation of linker for activation of T cells. Immunology, 157(4), 312-321.

Arbulo-Echevarria, M. M., Narbona-Sánchez, I., Fernandez-Ponce, C. M., Vico-Barranco, I., Rueda-Ygueravide, M. D., Dustin, M. L., ... & Aguado, E. (2018). A stretch of negatively charged amino acids of linker for activation of t-cell adaptor has a dual role in t-cell antigen receptor intracellular signaling. Frontiers in immunology, 9, 115.

Carpier, J. M., Zucchetti, A. E., Bataille, L., Dogniaux, S., Shafaq-Zadah, M., Bardin, S., ... & Hivroz, C. (2018). Rab6-dependent retrograde traffic of LAT controls immune synapse formation and T cell activation. Journal of Experimental Medicine, 215(4), 1245-1265.

Wang, L. N., Gao, M. H., Wang, B., Cong, B. B., & Zhang, S. C. (2018). A role for GPI-CD59 in promoting T-cell signal transduction via LAT. Oncology Letters, 15(4), 4873-4881.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

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For research use only. Not intended for any clinical use.

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