Mouse Anti-LDB1 Antibody (1C5) (CBMAB-0611-YC)

Provided herein are mouse monoclonal antibodies against Human LDB1. The antibody clone 1C5 can be used for immunoassay techniques, such as IP and MA.
See all LDB1 antibodies

Datasheet

Summary

Host Animal

Mouse

Specificity

Human

Clone

1C5

Antibody Isotype

IgG2b

Application

IP, MA

Basic Information

Immunogen

Recombinant protein

Specificity

Human

Antibody Isotype

IgG2b

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Supernatant

Storage

Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name

LDB1

Introduction

LDB1 (LIM domain binding 1) is a protein that in humans is encoded by the LDB1 gene. LDB1 has been shown to interact with LMO4, TCF3, TAL1 and CBFA2T3.

Entrez Gene ID

8861

UniProt ID

Q86U70

Alternative Names

NLI; CLIM2; LDB-1; CLIM-2

Function

Binds to the LIM domain of a wide variety of LIM domain-containing transcription factors. May regulate the transcriptional activity of LIM-containing proteins by determining specific partner interactions. Plays a role in the development of interneurons and motor neurons in cooperation with LHX3 and ISL1. Acts synergistically with LHX1/LIM1 in axis formation and activation of gene expression. Acts with LMO2 in the regulation of red blood cell development, maintaining erythroid precursors in an immature state (By similarity).

Biological Process

Anterior/posterior axis specificationIEA:Ensembl
Cellular component assemblyIEA:Ensembl
Cerebellar Purkinje cell differentiationIEA:Ensembl
Epithelial structure maintenanceIEA:Ensembl
Gastrulation with mouth forming secondIEA:Ensembl
Hair follicle developmentIEA:Ensembl
Histone H3-K4 acetylationISS:BHF-UCL
Mesendoderm developmentIEA:Ensembl
Negative regulation of erythrocyte differentiationISS:UniProtKB
Negative regulation of transcription by RNA polymerase IIManual Assertion Based On ExperimentIBA:GO_Central
Negative regulation of transcription, DNA-templatedManual Assertion Based On ExperimentIDA:UniProtKB
Nervous system developmentManual Assertion Based On ExperimentIBA:GO_Central
Neuron differentiationISS:UniProtKB
Positive regulation of cell adhesionIEA:Ensembl
Positive regulation of hemoglobin biosynthetic processISS:BHF-UCL
Positive regulation of transcription by RNA polymerase IIManual Assertion Based On ExperimentIMP:BHF-UCL
Regulation of cell migrationISS:UniProtKB
Regulation of focal adhesion assemblyISS:UniProtKB
Regulation of kinase activityISS:UniProtKB
Regulation of transcription elongation from RNA polymerase II promoterISS:BHF-UCL
Somatic stem cell population maintenanceIEA:Ensembl
Transcription by RNA polymerase IIIEA:Ensembl
Transcription-dependent tethering of RNA polymerase II gene DNA at nuclear peripheryISS:BHF-UCL
Wnt signaling pathwayIEA:Ensembl

Cellular Location

Nucleus

PTM

Ubiquitinated by RLIM/RNF12, leading to its degradation by the proteasome.

References

Zhu, M., Jiang, B., Zuo, H., Wang, X., Ge, H., & Huang, Z. (2022). LIM-Domain-Binding Protein 1 Mediates Cell Proliferation and Drug Resistance in Colorectal Cancer. Frontiers in Surgery, 8, 790380.

Wang, H., Kim, J., Wang, Z., Yan, X. X., Dean, A., & Xu, W. (2020). Crystal structure of human LDB1 in complex with SSBP2. Proceedings of the National Academy of Sciences, 117(2), 1042-1048.

Huang, Z., Yang, P., Ge, H., Yang, C., Cai, Y., Chen, Z., ... & Wang, H. (2020). RING finger protein 38 mediates LIM domain binding 1 degradation and regulates cell growth in colorectal cancer. OncoTargets and therapy, 371-379.

Liu, G., & Dean, A. (2019). Enhancer long-range contacts: The multi-adaptor protein LDB1 is the tie that binds. Biochimica et Biophysica Acta (BBA)-Gene Regulatory Mechanisms, 1862(6), 625-633.

Li, L., Mitra, A., Zhao, B., Choi, S., Lee, J., Stamos, D., ... & Dave, U. (2019). LIM Domain Protein 1 (Ldb1) Is Required for Lmo2 Oncogene-Induced Thymocyte Self-Renewal and T-Cell Leukemia in a Mouse Model of Human T-ALL.

Jorgensen-Muga, K. (2018). Functional role of LIM domain binding protein 1 in murine female reproductive physiology and uterine hemoglobin biosynthesis (Doctoral dissertation, Washington State University).

Ahmed, S., Pryce, B. R., Al-Zahrani, K. N., & Sabourin, L. A. (2018). The LIM domain binding protein 1, Ldb1, has distinct roles in Neu-induced mammary tumorigenesis. Biochimica et Biophysica Acta (BBA)-Molecular Cell Research, 1865(11), 1590-1597.

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Protocols

Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Associated Products

Related Products

Mouse Anti-LDB1 Recombinant Antibody (2G9)

Mouse Anti-LDB1 Recombinant Antibody (CBYCL-179)

Mouse Anti-LDB1 Recombinant Antibody (CBLC231-LY)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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