Mouse Anti-LDHC Recombinant Antibody (2C8) (CBMAB-L0971-YJ)

Provided herein is a Mouse monoclonal antibody, which binds to Lactate Dehydrogenase C (LDHC). The antibody can be used for immunoassay techniques, such as ELISA, IF.
See all LDHC antibodies

Datasheet

Summary

Host Animal

Mouse

Specificity

Human

Clone

2C8

Antibody Isotype

IgG2a, κ

Application

ELISA, IF

Basic Information

Immunogen

LDHC (NP_002292.1, 71 a.a. ~ 160 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.Immunogen sequence: FFSTSKITSG KDYSVSANSR IVIVTAGARQ QEGETRLALV QRNVAIMKSI IPAIVHYSPD CKILVVSNPV DILTYIVWKI SGLPVTRVIG.

Specificity

Human

Antibody Isotype

IgG2a, κ

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Buffer

PBS, pH 7.4

Storage

Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Epitope

aa 71-160

Target

Full Name

lactate dehydrogenase C

Introduction

Lactate dehydrogenase C catalyzes the conversion of L-lactate and NAD to pyruvate and NADH in the final step of anaerobic glycolysis. LDHC is testis-specific and belongs to the lactate dehydrogenase family. Two transcript variants have been detected which differ in the 5' untranslated region.

Entrez Gene ID

3948

UniProt ID

P07864

Alternative Names

CT32; LDH3; LDHX

Function

Possible role in sperm motility.

Biological Process

ATP biosynthetic processIEA:Ensembl
Carbohydrate metabolic processIEA:InterPro
Flagellated sperm motilityIEA:Ensembl
Lactate biosynthetic process from pyruvateIEA:Ensembl
Lactate oxidationIEA:Ensembl

Cellular Location

Cytoplasm

References

Naik, A., & Decock, J. (2022). Targeting of lactate dehydrogenase C dysregulates the cell cycle and sensitizes breast cancer cells to DNA damage response targeted therapy. Molecular Oncology, 16(4), 885-903.

Thomas, R., Shaath, H., Naik, A., Toor, S. M., Elkord, E., & Decock, J. (2020). Identification of two HLA-A* 0201 immunogenic epitopes of lactate dehydrogenase C (LDHC): potential novel targets for cancer immunotherapy. Cancer immunology, immunotherapy, 69, 449-463.

Dodo, M., Kitamura, H., Shima, H., Saigusa, D., Wati, S. M., Ota, N., ... & Motohashi, H. (2019). Lactate dehydrogenase C is required for the protein expression of a sperm-specific isoform of lactate dehydrogenase A. The journal of biochemistry, 165(4), 323-334.

Q & As

Ask a question We look forward to hearing from you.

0 reviews or Q&As

Purchasing FAQs
Technical FAQs

Review & reward

Have you used Mouse Anti-LDHC Recombinant Antibody (2C8)?
Submit a review and get a Coupon or an Amazon gift card. 20% off Coupon

$30 eGift Card
Submit a review

Protocols

Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Associated Products

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

Online Inquiry

Documents

Datasheet
SDS
Request for COA

Request bulk or custom quote

Second antibody and isotype control

Contact us

Tel: (USA)
(UK)
Fax:

Global Locations

Email: