Rabbit Anti-LDHC Recombinant Antibody (
9F2) (V2LY-0725-LY1434)

Name: Rabbit Anti-LDHC Recombinant Antibody (9F2)
SKU: V2LY-0725-LY1434
Rating: 5 (1 reviews)

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Tested Data

Rabbit Anti-LDHC Recombinant Antibody (9F2) (V2LY-0725-LY1434) in FC

Overlay Peak curve showing Hela cells stained with V2LY-0725-LY1434 (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*106cells) for 45min at 4°C. The secondary antibody used was FITC-conjugated Goat Anti-rabbit IgG(H+L) at 1:200 dilution for 35min at 4°C.Control antibody (green line) was rabbit IgG (1ug/1*106cells) used under the same conditions. Acquisition of>10,000 events was performed.

Datasheet

SDS

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Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Rabbit

Clone

9F2

Application

ELISA, FC

Immunogen

A synthesized peptide derived from human LDHC.

Host Species

Rabbit

Specificity

Human

Antibody Isotype

IgG

Clonality

Monoclonal Antibody

Application Notes

Application	Note
FC	1:50-1:200

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, glycerol

Preservative

Sodium azide

Concentration

Batch dependent

Purity

> 95% Purity determined by SDS-PAGE.

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

More Infomation

Target

Full Name

lactate dehydrogenase C

Entrez Gene ID

3948

UniProt ID

P07864

Function

Possible role in sperm motility.

Biological Process

ATP biosynthetic processIEA:Ensembl
Carbohydrate metabolic processIEA:InterPro
Flagellated sperm motilityIEA:Ensembl
Lactate biosynthetic process from pyruvateIEA:Ensembl
Lactate oxidationIEA:Ensembl

Cellular Location

Cytoplasm

Naik, A., & Decock, J. (2022). Targeting of lactate dehydrogenase C dysregulates the cell cycle and sensitizes breast cancer cells to DNA damage response targeted therapy. Molecular Oncology, 16(4), 885-903.

Thomas, R., Shaath, H., Naik, A., Toor, S. M., Elkord, E., & Decock, J. (2020). Identification of two HLA-A* 0201 immunogenic epitopes of lactate dehydrogenase C (LDHC): potential novel targets for cancer immunotherapy. Cancer immunology, immunotherapy, 69, 449-463.

Dodo, M., Kitamura, H., Shima, H., Saigusa, D., Wati, S. M., Ota, N., ... & Motohashi, H. (2019). Lactate dehydrogenase C is required for the protein expression of a sperm-specific isoform of lactate dehydrogenase A. The journal of biochemistry, 165(4), 323-334.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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