Mouse Anti-LFNG Recombinant Antibody (1F9) (CBMAB-A4930-LY)

Name: Mouse Anti-LFNG Recombinant Antibody (1F9)
SKU: CBMAB-A4930-LY
Rating: 5 (1 reviews)

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Datasheet

SDS

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Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

1F9

Application

WB, ELISA

Immunogen

LFNG (AAH14851, 1 a.a. ~ 250 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.

Specificity

Human, Mouse

Antibody Isotype

IgG2a, κ

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Purity

> 95% Purity determined by SDS-PAGE.

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

More Infomation

Target

Full Name

LFNG O-fucosylpeptide 3-beta-N-acetylglucosaminyltransferase

Introduction

This gene encodes a member of the glycosyltransferase superfamily. The encoded protein is a single-pass type II Golgi membrane protein that functions as a fucose-specific glycosyltransferase, adding an N-acetylglucosamine to the fucose residue of a group of signaling receptors involved in regulating cell fate decisions during development. Mutations in this gene have been associated with autosomal recessive spondylocostal dysostosis 3. Alternatively spliced transcript variants that encode different isoforms have been described, however, not all variants have been fully characterized. [provided by RefSeq]

Entrez Gene ID

Human	3955
Mouse	16848

UniProt ID

Human	Q8NES3
Mouse	O09010

Alternative Names

SCDO3

Function

Glycosyltransferase that initiates the elongation of O-linked fucose residues attached to EGF-like repeats in the extracellular domain of Notch molecules. Modulates NOTCH1 activity by modifying O-fucose residues at specific EGF-like domains resulting in inhibition of NOTCH1 activation by JAG1 and enhancement of NOTCH1 activation by DLL1 via an increase in its binding to DLL1 (By similarity).
Decreases the binding of JAG1 to NOTCH2 but not that of DLL1 (PubMed:11346656).
Essential mediator of somite segmentation and patterning (By similarity).

Biological Process

Animal organ morphogenesis1 PublicationNAS:UniProtKB
Compartment pattern specificationIEA:Ensembl
Marginal zone B cell differentiationISS:UniProtKB
Negative regulation of Notch signaling pathway involved in somitogenesisISS:UniProtKB
Ovarian follicle developmentIEA:Ensembl
Positive regulation of meiotic cell cycleIEA:Ensembl
Positive regulation of Notch signaling pathwayIEA:Ensembl
Positive regulation of protein bindingIEA:Ensembl
Regulation of Notch signaling pathwayISS:CAFA
Regulation of somitogenesisManual Assertion Based On ExperimentIMP:UniProtKB
SomitogenesisISS:UniProtKB
T cell differentiationISS:UniProtKB

Cellular Location

Golgi apparatus membrane

Involvement in disease

Spondylocostal dysostosis 3, autosomal recessive (SCDO3):
A condition of variable severity associated with vertebral and rib segmentation defects. The main skeletal malformations include fusion of vertebrae, hemivertebrae, fusion of certain ribs, and other rib malformations. Deformity of the chest and spine (severe scoliosis, kyphoscoliosis and lordosis) is a natural consequence of the malformation and leads to a dwarf-like appearance. As the thorax is small, infants frequently have respiratory insufficiency and repeated respiratory infections resulting in life-threatening complications in the first year of life.

Topology

Cytoplasmic: 1-8
Helical: 9-29
Lumenal: 30-379

PTM

A soluble form may be derived from the membrane form by proteolytic processing.

Otomo, N., Mizumoto, S., Lu, H. F., Takeda, K., Campos-Xavier, B., Mittaz-Crettol, L., ... & Ikegawa, S. (2019). Identification of novel LFNG mutations in spondylocostal dysostosis. Journal of human genetics, 64(3), 261-264.

Del Castillo Velasco‐Herrera, M., van der Weyden, L., Nsengimana, J., Speak, A. O., Sjöberg, M. K., Bishop, D. T., ... & Adams, D. J. (2018). Comparative genomics reveals that loss of lunatic fringe (LFNG) promotes melanoma metastasis. Molecular Oncology, 12(2), 239-255.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

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