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Mouse Anti-LIMD1 Recombinant Antibody (CBYJL-1707) (CBMAB-L1587-YJ)

Provided herein is a Mouse monoclonal antibody, which binds to Lim Domains Containing 1 (LIMD1). The antibody can be used for immunoassay techniques, such as ELISA, IHC, WB.
See all LIMD1 antibodies

Summary

Host Animal
Mouse
Specificity
Human
Clone
CBYJL-1707
Antibody Isotype
IgG2b, κ
Application
ELISA, IHC, WB

Basic Information

Immunogen
Partial recombinant corresponding to aa 577-675 from LIMD1 (NP_055055.1) with GST tag. MW of the GST tag alone is 26kD.
Specificity
Human
Antibody Isotype
IgG2b, κ
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Buffer
PBS, pH 7.4
Storage
Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Epitope
aa 577-675

Target

Full Name
LIM Domains Containing 1
Introduction
Among its related pathways are Cytoskeletal Signaling and CDK-mediated phosphorylation and removal of Cdc6. Gene Ontology (GO) annotations related to this gene include transcription corepressor activity. An important paralog of this gene is WTIP. Adapter or scaffold protein which participates in the assembly of numerous protein complexes and is involved in several cellular processes such as cell fate determination, cytoskeletal organization, repression of gene transcription, cell-cell adhesion, cell differentiation, proliferation and migration. Positively regulates microRNA (miRNA)-mediated gene silencing and is essential for P-body formation and integrity. LIMD1 acts as a hypoxic regulator by bridging an association between the prolyl hydroxylases and VHL enabling efficient degradation of HIF1A. Acts as a transcriptional corepressor for SNAI1- and SNAI2/SLUG-dependent repression of E-cadherin transcription. It negatively regulates the Hippo signaling pathway and antagonizes phosphorylation of YAP1. LIMD1 inhibits E2F-mediated transcription, and suppresses the expression of the majority of genes with E2F1-responsive elements. LIMD1 regulates osteoblast development, function, differentiation and stress osteoclastogenesis. Enhances the ability of TRAF6 to activate adapter protein complex 1 (AP-1) and negatively regulates the canonical Wnt receptor signaling pathway in osteoblasts. LIMD1 may act as a tumor suppressor by inhibiting cell proliferation.
Entrez Gene ID
8994
UniProt ID
Q9UGP4
Function
Adapter or scaffold protein which participates in the assembly of numerous protein complexes and is involved in several cellular processes such as cell fate determination, cytoskeletal organization, repression of gene transcription, cell-cell adhesion, cell differentiation, proliferation and migration. Positively regulates microRNA (miRNA)-mediated gene silencing and is essential for P-body formation and integrity. Acts as a hypoxic regulator by bridging an association between the prolyl hydroxylases and VHL enabling efficient degradation of HIF1A. Acts as a transcriptional corepressor for SNAI1- and SNAI2/SLUG-dependent repression of E-cadherin transcription. Negatively regulates the Hippo signaling pathway and antagonizes phosphorylation of YAP1. Inhibits E2F-mediated transcription, and suppresses the expression of the majority of genes with E2F1-responsive elements. Regulates osteoblast development, function, differentiation and stress osteoclastogenesis. Enhances the ability of TRAF6 to activate adapter protein complex 1 (AP-1) and negatively regulates the canonical Wnt receptor signaling pathway in osteoblasts. May act as a tumor suppressor by inhibiting cell proliferation.
Biological Process
Cell migrationManual Assertion Based On ExperimentIMP:UniProtKB
Cytoskeleton organizationManual Assertion Based On ExperimentIMP:UniProtKB
Gene silencing by miRNAManual Assertion Based On ExperimentIMP:MGI
Negative regulation of canonical Wnt signaling pathwayISS:UniProtKB
Negative regulation of hippo signalingManual Assertion Based On ExperimentIDA:UniProtKB
Negative regulation of osteoblast differentiationISS:UniProtKB
Negative regulation of transcription, DNA-templatedManual Assertion Based On ExperimentIDA:UniProtKB
Osteoblast developmentISS:UniProtKB
P-body assemblyManual Assertion Based On ExperimentIMP:MGI
PhosphorylationManual Assertion Based On ExperimentIDA:UniProtKB
Positive regulation of gene silencing by miRNAManual Assertion Based On ExperimentIMP:UniProtKB
Regulation of cell shapeManual Assertion Based On ExperimentIMP:UniProtKB
Regulation of transcription, DNA-templatedManual Assertion Based On ExperimentIBA:GO_Central
Response to hypoxiaManual Assertion Based On ExperimentIDA:UniProtKB
Cellular Location
Cytoplasm
Nucleus
Cytoplasm, P-body
Cell junction, adherens junction
Cell junction, focal adhesion
Shuttles between cytoplasm and nucleus but is localized predominantly to the cytoplasm. Found in the nucleus but not nucleoli. Colocalizes with VCL in the focal adhesions. Down-regulation and/or elimination of its expression from the nucleus of neoplastic cells correlates strongly with poor patient prognosis and aggressive forms of breast carcinoma. Conversely, strong nuclear localization correlates with low-tumor grade and better patient prognosis.
PTM
Phosphorylated during mitosis.

Li, W. H., Huang, K., Wen, F. B., Cui, G. H., Guo, H. Z., & Zhao, S. (2022). PLOD3 regulates the expression of YAP1 to affect the progression of non-small cell lung cancer via the PKCδ/CDK1/LIMD1 signaling pathway. Laboratory Investigation, 102(4), 440-451.

Wang, Y., Zhang, C., Yang, W., Shao, S., Xu, X., Sun, Y., ... & Wu, C. (2021). LIMD1 phase separation contributes to cellular mechanics and durotaxis by regulating focal adhesion dynamics in response to force. Developmental Cell, 56(9), 1313-1325.

Landry, N. M., Rattan, S. G., Filomeno, K. L., Meier, T. W., Meier, S. C., Foran, S. J., ... & Dixon, I. M. (2021). SKI activates the Hippo pathway via LIMD1 to inhibit cardiac fibroblast activation. Basic Research in Cardiology, 116, 1-27.

Zeng, X., Wang, H., He, D., Jia, W., & Ma, R. (2020). LIMD1 increases the sensitivity of lung adenocarcinoma cells to cisplatin via the GADD45α/p38 MAPK signaling pathway. Frontiers in Oncology, 10, 969.

Pan, J., Tang, Y., Liu, S., Li, L., Yu, B., Lu, Y., & Wang, Y. (2020). LIMD1‐AS1 suppressed non‐small cell lung cancer progression through stabilizing LIMD1 mRNA via hnRNP U. Cancer Medicine, 9(11), 3829-3839.

Zhou, J., Zhang, L., Zhou, W., Chen, Y., Cheng, Y., & Dong, J. (2019). LIMD 1 phosphorylation in mitosis is required for mitotic progression and its tumor‐suppressing activity. The FEBS Journal, 286(5), 963-974.

Wang, L., Howell, M. E., McPeak, B., Riggs, K., Kohne, C., Yohanon, J. U., ... & Ning, S. (2018). LIMD1 is induced by and required for LMP1 signaling, and protects EBV-transformed cells from DNA damage-induced cell death. Oncotarget, 9(5), 6282.

Zhang, D., Li, S., Yu, W., Chen, C., Liu, T., Sun, Y., ... & Liu, L. (2018). LIMD1 is a survival prognostic marker of gastric cancer and hinders tumor progression by suppressing activation of YAP1. Cancer Management and Research, 4349-4361.

Ibar, C., Kirichenko, E., Keepers, B., Enners, E., Fleisch, K., & Irvine, K. D. (2018). Tension-dependent regulation of mammalian Hippo signaling through LIMD1. Journal of Cell Science, 131(5), jcs214700.

Foxler, D. E., Bridge, K. S., Foster, J. G., Grevitt, P., Curry, S., Shah, K. M., ... & Sharp, T. V. (2018). A HIF–LIMD 1 negative feedback mechanism mitigates the pro‐tumorigenic effects of hypoxia. EMBO molecular medicine, 10(8), e8304.

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For research use only. Not intended for any clinical use.

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