Mouse Anti-LIME1 Recombinant Antibody (CBYJL-1710) (CBMAB-L1591-YJ)

Name: Mouse Anti-LIME1 Recombinant Antibody (CBYJL-1710)
SKU: CBMAB-L1591-YJ
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

CBYJL-1710

Application

IHC-P, WB

Specificity

Human

Antibody Isotype

IgG1

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Buffer

PBS, pH 7.3, 1% BSA, 50% Glycerol

Preservative

0.02% Sodium Azide

Storage

Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Epitope

aa 30-295

More Infomation

Target

Full Name

Lck interacting transmembrane adaptor 1

Introduction

LIME1 is a transmembrane adaptor protein that links the T and B-cell receptor stimulation to downstream signaling pathways via its association with the Src family kinases Lck and Lyn, respectively. Alternative splicing of this gene results in multiple transcript variants. LIME1 involved in BCR (B-cell antigen receptor)-mediated signaling in B-cells and TCR (T-cell antigen receptor)-mediated T-cell signaling in T-cells. In absence of TCR signaling, may be involved in CD4-mediated inhibition of T-cell activation. Couples activation of these receptors and their associated kinases with distal intracellular events such as calcium mobilization or MAPK activation through the recruitment of PLCG2, GRB2, GRAP2, and other signaling molecules.

Entrez Gene ID

54923

UniProt ID

Q9H400

Alternative Names

LIME; dJ583P15.4

Function

Involved in BCR (B-cell antigen receptor)-mediated signaling in B-cells and TCR (T-cell antigen receptor)-mediated T-cell signaling in T-cells. In absence of TCR signaling, may be involved in CD4-mediated inhibition of T-cell activation. Couples activation of these receptors and their associated kinases with distal intracellular events such as calcium mobilization or MAPK activation through the recruitment of PLCG2, GRB2, GRAP2, and other signaling molecules.

Biological Process

Adaptive immune responseIEA:UniProtKB-KW
B cell receptor signaling pathwayManual Assertion Based On ExperimentIBA:GO_Central
Regulation of I-kappaB kinase/NF-kappaB signalingManual Assertion Based On ExperimentIBA:GO_Central
Regulation of MAP kinase activityManual Assertion Based On ExperimentIBA:GO_Central
Regulation of NIK/NF-kappaB signalingManual Assertion Based On ExperimentIBA:GO_Central
Regulation of phosphatidylinositol 3-kinase signalingManual Assertion Based On ExperimentIBA:GO_Central
Regulation of release of sequestered calcium ion into cytosolManual Assertion Based On ExperimentIBA:GO_Central
Regulation of transcription by RNA polymerase IIManual Assertion Based On ExperimentIBA:GO_Central
T cell receptor signaling pathwayIEA:InterPro

Cellular Location

Cell membrane
Present in lipid rafts. Recruited to the immunological synapse upon conjugation of T-cell with antigen-presenting cell.

Topology

Extracellular: 1-6
Helical: 7-27
Cytoplasmic: 28-295

PTM

Palmitoylation of Cys-28 and Cys-31 is required for raft targeting.
Phosphorylated on tyrosines upon TCR activation and/or CD4 coreceptor stimulation, or upon BCR stimulation; which leads to the recruitment of SH2-containing proteins.

Li, S. C., Kuo, H. C., Huang, L. H., Chou, W. J., Lee, S. Y., Chan, W. C., & Wang, L. J. (2021). DNA methylation in LIME1 and SPTBN2 genes is associated with attention deficit in children. Children, 8(2), 92.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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Mouse Anti-LIME1 Recombinant Antibody (CBYCL-335) (CAT#: CBMAB-L0236-YC)

Mouse Anti-LIME1 Recombinant Antibody (CBYJL-1711) (CAT#: CBMAB-L1593-YJ)

Mouse Anti-LIME1 Recombinant Antibody (CBYCL-334) (CAT#: CBMAB-L0235-YC)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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