Mouse Anti-LMCD1 Recombinant Antibody (2F12) (CBMAB-L1754-YJ)

Name: Mouse Anti-LMCD1 Recombinant Antibody (2F12)
SKU: CBMAB-L1754-YJ
Rating: 5 (1 reviews)

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Datasheet

SDS

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Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

2F12

Application

FC, WB

Specificity

Human

Antibody Isotype

IgG1

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Buffer

PBS, pH 7.3, 1% BSA, 50% Glycerol

Preservative

0.02% Sodium Azide

Storage

Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

More Infomation

Target

Full Name

LIM and cysteine rich domains 1

Introduction

LMCD1 is a member of the LIM-domain family of zinc finger proteins. LMCD1 contains an N-terminal cysteine-rich domain and two C-terminal LIM domains. The presence of LIM domains suggests involvement in protein-protein interactions. LMCD1 may act as a co-regulator of transcription along with other transcription factors. Alternate splicing results in multiple transcript variants of this gene. Among its related pathways are Surfactant metabolism and Metabolism of proteins. Gene Ontology (GO) annotations related to this gene include transcription corepressor activity. An important paralog of this gene is TES.

Entrez Gene ID

29995

UniProt ID

Q9NZU5

Alternative Names

LIM And Cysteine Rich Domains 1 2 3 5Dyxin 2 3 4LIM And Cysteine-Rich Domains Protein 1 3

Function

Transcriptional cofactor that restricts GATA6 function by inhibiting DNA-binding, resulting in repression of GATA6 transcriptional activation of downstream target genes. Represses GATA6-mediated trans activation of lung- and cardiac tissue-specific promoters. Inhibits DNA-binding by GATA4 and GATA1 to the cTNC promoter (By similarity).
Plays a critical role in the development of cardiac hypertrophy via activation of calcineurin/nuclear factor of activated T-cells signaling pathway.

Biological Process

Negative regulation of transcription by RNA polymerase IIIEA:Ensembl
Positive regulation of calcineurin-NFAT signaling cascadeManual Assertion Based On ExperimentIMP:UniProtKB
Regulation of cardiac muscle hypertrophyManual Assertion Based On ExperimentIMP:UniProtKB

Cellular Location

Cytoplasm
Nucleus
May shuttle between the cytoplasm and the nucleus.

Bogatkevich, G. S., Atanelishvili, I., Bogatkevich, A. M., & Silver, R. M. (2023). Critical Role of LMCD1 in Promoting Profibrotic Characteristics of Lung Myofibroblasts in Experimental and Scleroderma‐Associated Lung Fibrosis. Arthritis & Rheumatology, 75(3), 438-448.

Ye, Z., Chen, G., Hou, C., Jiang, Z., Wang, E., & Wang, J. (2022). LMCD1 facilitates the induction of pluripotency via cell proliferation, metabolism, and epithelial‐mesenchymal transition. Cell Biology International, 46(9), 1409-1422.

Yu, R., Tian, M., He, P., Chen, J., Zhao, Z., Zhang, Y., & Zhang, B. (2022). Suppression of LMCD1 ameliorates renal fibrosis by blocking the activation of ERK pathway. Biochimica et Biophysica Acta (BBA)-Molecular Cell Research, 1869(4), 119200.

He, J. W., Li, D. J., Zhou, J. H., Zhu, Y. L., & Yu, B. Q. (2020). SP1-mediated upregulation of lncRNA LMCD1-AS1 functions a ceRNA for miR-106b-5p to facilitate osteosarcoma progression. Biochemical and Biophysical Research Communications, 526(3), 670-677.

Jin, X., Zhang, S., Ding, T., Zhao, P., Zhang, C., Zhang, Y., & Li, W. (2020). Testicular Lmcd1 regulates phagocytosis by Sertoli cells through modulation of NFAT1/Txlna signaling pathway. Aging Cell, 19(10), e13217.

Shao, J., Xu, Y., Li, H., Chen, L., Wang, W., Shen, D., & Chen, J. (2020). LMCD1 antisense RNA 1 (LMCD1-AS1) potentiates thyroid cancer cell growth and stemness via a positive feedback loop of LMCD1-AS1/miR-1287-5p/GLI2. Annals of Translational Medicine, 8(22).

Yu, J., Zhang, B., Zhang, H., Qi, Y., Wang, Y., Wang, W., ... & Wang, Y. (2019). E2F1-induced upregulation of long non-coding RNA LMCD1-AS1 facilitates cholangiocarcinoma cell progression by regulating miR-345–5p/COL6A3 pathway. Biochemical and biophysical research communications, 512(2), 150-155.

Zhu, B., Xue, F., Zhang, C., & Li, G. (2019). LMCD1 promotes osteogenic differentiation of human bone marrow stem cells by regulating BMP signaling. Cell death & disease, 10(9), 647.

Ferreira, D. M., Cheng, A. J., Agudelo, L. Z., Cervenka, I., Chaillou, T., Correia, J. C., ... & Ruas, J. L. (2019). LIM and cysteine-rich domains 1 (LMCD1) regulates skeletal muscle hypertrophy, calcium handling, and force. Skeletal Muscle, 9, 1-19.

Wang, T., Fu, X., Jin, T., Zhang, L., Liu, B., Wu, Y., ... & Ye, L. (2019). Aspirin targets P4HA2 through inhibiting NF-κB and LMCD1-AS1/let-7g to inhibit tumour growth and collagen deposition in hepatocellular carcinoma. EBioMedicine, 45, 168-180.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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For research use only. Not intended for any clinical use.

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