Mouse Anti-LMO3 Recombinant Antibody (3G7) (CBMAB-A5024-LY)

The product is antibody recognizes LMO3. The antibody 3G7 immunoassay techniques such as: WB, ELISA.
See all LMO3 antibodies

Datasheet

Summary

Host Animal

Mouse

Specificity

Human

Clone

3G7

Antibody Isotype

IgG2b, κ

Application

WB, ELISA

Basic Information

Immunogen

LMO3 (NP_061110, 91 a.a. ~ 145 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.

Specificity

Human

Antibody Isotype

IgG2b, κ

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Purity

> 95% Purity determined by SDS-PAGE.

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name

LIM domain only 3 (rhombotin-like 2)

Entrez Gene ID

55885

UniProt ID

Q8TAP4

Alternative Names

DAT1; MGC26081; RBTN3; RBTNL2; RHOM3; Rhom-3

Biological Process

Negative regulation of ERK1 and ERK2 cascadeManual Assertion Based On ExperimentIDA:UniProtKB
Positive regulation of fat cell differentiationManual Assertion Based On ExperimentIMP:UniProtKB
Positive regulation of glucocorticoid receptor signaling pathwayManual Assertion Based On ExperimentIDA:UniProtKB
Positive regulation of peroxisome proliferator activated receptor signaling pathwayManual Assertion Based On ExperimentIDA:UniProtKB

Cellular Location

Cytoplasm

References

Ohara, Y., Craig, A. J., Liu, H., Yang, S., Moreno, P., Dorsey, T. H., ... & Hussain, S. P. (2023). LMO3 is a suppressor of the basal-like/squamous PDAC subtype and reduces disease aggressiveness of pancreatic cancer through glycerol 3-phosphate metabolism. bioRxiv, 2023-11.

Ling, Z., Long, X., Wu, Y., Li, J., & Feng, M. (2022). LMO3 promotes proliferation and metastasis of papillary thyroid carcinoma cells by regulating LIMK1-mediated cofilin and the β-catenin pathway. Open Medicine, 17(1), 453-462.

Wagner, G., Fenzl, A., Lindroos-Christensen, J., Einwallner, E., Husa, J., Witzeneder, N., ... & Bilban, M. (2021). LMO3 reprograms visceral adipocyte metabolism during obesity. Journal of Molecular Medicine, 99(8), 1151-1171.

Savarese, A., & Lasek, A. W. (2018). Regulation of anxiety-like behavior and Crhr1 expression in the basolateral amygdala by LMO3. Psychoneuroendocrinology, 92, 13-20.

Qiu, Y. S., Jiang, N. N., Zhou, Y., Yu, K. Y., Gong, H. Y., & Liao, G. J. (2018). LMO3 promotes gastric cancer cell invasion and proliferation through Akt-mTOR and Akt-GSK3β signaling. International journal of molecular medicine, 41(5), 2755-2763.

Cheng, Y., Hou, T., Ping, J., Chen, T., & Yin, B. (2018). LMO3 promotes hepatocellular carcinoma invasion, metastasis and anoikis inhibition by directly interacting with LATS1 and suppressing Hippo signaling. Journal of Experimental & Clinical Cancer Research, 37(1), 1-12.

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Protocols

Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Associated Products

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Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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