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Mouse Anti-LMX1B Recombinant Antibody (50.5A5) (CBMAB-0233-CN)

This product is a mouse antibody that recognizes LMX1B of chicken. The antibody 50.5A5 can be used for immunoassay techniques such as: IF, IHC.
See all LMX1B antibodies

Summary

Host Animal
Mouse
Specificity
Chicken, Mouse, Planaria
Clone
50.5A5
Antibody Isotype
IgG1, κ
Application
IF, IHC

Basic Information

Immunogen
Recombinant 6× His-tagged LMX1 protein fragment (LIM domains and homeodomain)
Specificity
Chicken, Mouse, Planaria
Antibody Isotype
IgG1, κ
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Purity
>95%, as determined by SDS-PAGE analysis
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
LMX1B
Introduction
This gene encodes a member of LIM-homeodomain family of proteins containing two N-terminal zinc-binding LIM domains, 1 homeodomain, and a C-terminal glutamine-rich domain. It functions as a transcription factor, and is essential for the normal development of dorsal limb structures, the glomerular basement membrane, the anterior segment of the eye, and dopaminergic and serotonergic neurons. The protein specifies dorsal cell fate during limb development.
Entrez Gene ID
Chicken396312
Mouse16917
UniProt ID
ChickenP53413
MouseO88609
Alternative Names
LMX; LMX1
Function
Transcription factor involved in the regulation of podocyte-expressed genes (PubMed:24042019, PubMed:28059119).
Essential for the specification of dorsal limb fate at both the zeugopodal and autopodal levels.
Biological Process
Dopaminergic neuron differentiationISS:ParkinsonsUK-UCL
Dorsal/ventral pattern formationISS:UniProtKB
Neuron differentiationISS:UniProtKB
Positive regulation of transcription by RNA polymerase IIISS:ParkinsonsUK-UCL
Regulation of transcription by RNA polymerase IIManual Assertion Based On ExperimentIBA:GO_Central
Regulation of transcription, DNA-templatedManual Assertion Based On ExperimentIDA:UniProtKB
Cellular Location
Nucleus
Involvement in disease
Nail-patella syndrome (NPS):
Disease that cause abnormal skeletal patterning and renal dysplasia.
Focal segmental glomerulosclerosis 10 (FSGS10):
An autosomal dominant form of focal segmental glomerulosclerosis, a renal pathology defined by the presence of segmental sclerosis in glomeruli and resulting in proteinuria, reduced glomerular filtration rate and progressive decline in renal function. Renal insufficiency often progresses to end-stage renal disease, a highly morbid state requiring either dialysis therapy or kidney transplantation.

Pereira, A. C., Dasgupta, K., Ho, T. V., Pacheco-Vergara, M., Kim, J., Kataria, N., ... & Jeong, J. (2023). Lineage-specific mutation of Lmx1b provides new insights into distinct regulation of suture development in different areas of the calvaria. Frontiers in Physiology, 14.

Kournoutis, A., & Johansen, T. (2023). LC3B is a cofactor for LMX1B-mediated transcription of autophagy genes in dopaminergic neurons. Journal of Cell Biology, 222(5), e202303008.

Jiménez-Moreno, N., Kollareddy, M., Stathakos, P., Moss, J. J., Antón, Z., Shoemark, D. K., ... & Lane, J. D. (2023). ATG8-dependent LMX1B-autophagy crosstalk shapes human midbrain dopaminergic neuronal resilience. Journal of Cell Biology, 222(5), e201910133.

Kim, K., Kim, J. H., Kim, I., Seong, S., Han, J. E., Lee, K. B., ... & Kim, N. (2022). Transcription factor Lmx1b negatively regulates osteoblast differentiation and bone formation. International Journal of Molecular Sciences, 23(9), 5225.

Chizhikov, V. V., Iskusnykh, I. Y., Fattakhov, N., & Fritzsch, B. (2021). Lmx1a and Lmx1b are redundantly required for the development of multiple components of the mammalian auditory system. Neuroscience, 452, 247-264.

Meer, E., Qin, V. L., Gudiseva, H. V., McGeehan, B., Salowe, R., Pistilli, M., ... & O’Brien, J. M. (2021). LMX1B locus associated with low-risk baseline glaucomatous features in the POAAGG study. Genes, 12(8), 1252.

Tolman, N. G., Balasubramanian, R., Macalinao, D. G., Kearney, A. L., MacNicoll, K. H., Montgomery, C. L., ... & John, S. W. (2021). Genetic background modifies vulnerability to glaucoma-related phenotypes in Lmx1b mutant mice. Disease Models & Mechanisms, 14(2), dmm046953.

Haro, E., Petit, F., Pira, C. U., Spady, C. D., Lucas-Toca, S., Yorozuya, L. I., ... & Oberg, K. C. (2021). Identification of limb-specific Lmx1b auto-regulatory modules with Nail-patella syndrome pathogenicity. Nature communications, 12(1), 5533.

Lei, L., Oh, G., Sutherland, S., Abra, G., Higgins, J., Sibley, R., ... & Kambham, N. (2020). Myelin bodies in LMX1B-associated nephropathy: potential for misdiagnosis. Pediatric Nephrology, 35, 1647-1657.

Donovan, L. J., Spencer, W. C., Kitt, M. M., Eastman, B. A., Lobur, K. J., Jiao, K., ... & Deneris, E. S. (2019). Lmx1b is required at multiple stages to build expansive serotonergic axon architectures. Elife, 8, e48788.

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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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