Mouse Anti-LMX1B Recombinant Antibody (CBYJL-1891) (CBMAB-L1898-YJ)

Name: Mouse Anti-LMX1B Recombinant Antibody (CBYJL-1891)
SKU: CBMAB-L1898-YJ
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

CBYJL-1891

Application

ELISA, IHC-P, WB

Immunogen

LMX1B (NP_002307, 1 a.a. ~ 110 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.Immunogen sequence: MLDGIKMEEH ALRPGPATLG VLLGSDCPHP AVCEGCQRPI SDRFLMRVNE SSWHEECLQC AACQQALTTS CYFRDRKLYC KQDYQQLFAAKCSGCMEKIA PTEFVMRALE.

Specificity

Human

Antibody Isotype

IgG2a, κ

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Buffer

PBS, pH 7.4

Storage

Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Epitope

aa 1-110

More Infomation

Target

Full Name

LMX1B

Introduction

LMX1B is a member of LIM-homeodomain family of proteins containing two N-terminal zinc-binding LIM domains, 1 homeodomain, and a C-terminal glutamine-rich domain. It plays a role as a transcription factor, and is essential for the normal development of dorsal limb structures, the glomerular basement membrane, the anterior segment of the eye, and dopaminergic and serotonergic neurons. Mutations in this gene are associated with nail-patella syndrome. Alternatively spliced transcript variants encoding different isoforms have been described for this gene.

Entrez Gene ID

4010

UniProt ID

O60663

Alternative Names

NPS1; LMX1.2

Function

Transcription factor involved in the regulation of podocyte-expressed genes (PubMed:24042019, PubMed:28059119).
Essential for the specification of dorsal limb fate at both the zeugopodal and autopodal levels.

Biological Process

Dopaminergic neuron differentiationISS:ParkinsonsUK-UCL
Dorsal/ventral pattern formationISS:UniProtKB
Neuron differentiationISS:UniProtKB
Positive regulation of transcription by RNA polymerase IIISS:ParkinsonsUK-UCL
Regulation of transcription by RNA polymerase IIManual Assertion Based On ExperimentIBA:GO_Central
Regulation of transcription, DNA-templatedManual Assertion Based On ExperimentIDA:UniProtKB

Cellular Location

Nucleus

Involvement in disease

Nail-patella syndrome (NPS):
Disease that cause abnormal skeletal patterning and renal dysplasia.
Focal segmental glomerulosclerosis 10 (FSGS10):
An autosomal dominant form of focal segmental glomerulosclerosis, a renal pathology defined by the presence of segmental sclerosis in glomeruli and resulting in proteinuria, reduced glomerular filtration rate and progressive decline in renal function. Renal insufficiency often progresses to end-stage renal disease, a highly morbid state requiring either dialysis therapy or kidney transplantation.

Pereira, A. C., Dasgupta, K., Ho, T. V., Pacheco-Vergara, M., Kim, J., Kataria, N., ... & Jeong, J. (2023). Lineage-specific mutation of Lmx1b provides new insights into distinct regulation of suture development in different areas of the calvaria. Frontiers in Physiology, 14.

Kournoutis, A., & Johansen, T. (2023). LC3B is a cofactor for LMX1B-mediated transcription of autophagy genes in dopaminergic neurons. Journal of Cell Biology, 222(5), e202303008.

Jiménez-Moreno, N., Kollareddy, M., Stathakos, P., Moss, J. J., Antón, Z., Shoemark, D. K., ... & Lane, J. D. (2023). ATG8-dependent LMX1B-autophagy crosstalk shapes human midbrain dopaminergic neuronal resilience. Journal of Cell Biology, 222(5), e201910133.

Kim, K., Kim, J. H., Kim, I., Seong, S., Han, J. E., Lee, K. B., ... & Kim, N. (2022). Transcription factor Lmx1b negatively regulates osteoblast differentiation and bone formation. International Journal of Molecular Sciences, 23(9), 5225.

Chizhikov, V. V., Iskusnykh, I. Y., Fattakhov, N., & Fritzsch, B. (2021). Lmx1a and Lmx1b are redundantly required for the development of multiple components of the mammalian auditory system. Neuroscience, 452, 247-264.

Meer, E., Qin, V. L., Gudiseva, H. V., McGeehan, B., Salowe, R., Pistilli, M., ... & O’Brien, J. M. (2021). LMX1B locus associated with low-risk baseline glaucomatous features in the POAAGG study. Genes, 12(8), 1252.

Tolman, N. G., Balasubramanian, R., Macalinao, D. G., Kearney, A. L., MacNicoll, K. H., Montgomery, C. L., ... & John, S. W. (2021). Genetic background modifies vulnerability to glaucoma-related phenotypes in Lmx1b mutant mice. Disease Models & Mechanisms, 14(2), dmm046953.

Haro, E., Petit, F., Pira, C. U., Spady, C. D., Lucas-Toca, S., Yorozuya, L. I., ... & Oberg, K. C. (2021). Identification of limb-specific Lmx1b auto-regulatory modules with Nail-patella syndrome pathogenicity. Nature communications, 12(1), 5533.

Lei, L., Oh, G., Sutherland, S., Abra, G., Higgins, J., Sibley, R., ... & Kambham, N. (2020). Myelin bodies in LMX1B-associated nephropathy: potential for misdiagnosis. Pediatric Nephrology, 35, 1647-1657.

Donovan, L. J., Spencer, W. C., Kitt, M. M., Eastman, B. A., Lobur, K. J., Jiao, K., ... & Deneris, E. S. (2019). Lmx1b is required at multiple stages to build expansive serotonergic axon architectures. Elife, 8, e48788.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

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