Rabbit Anti-LPXN Recombinant Antibody (CBYCL-448) (CBMAB-L0349-YC)

Provided herein is a Rabbit monoclonal antibody against Human LPXN. The antibody can be used for immunoassay techniques, such as WB, IP.
See all LPXN antibodies

Datasheet

Summary

Host Animal

Rabbit

Specificity

Human

Clone

CBYCL-448

Antibody Isotype

IgG

Application

WB, IP

Basic Information

Specificity

Human

Antibody Isotype

IgG

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Storage

Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name

leupaxin

Introduction

LPXN is preferentially expressed in hematopoietic cells and belongs to the paxillin protein family. Similar to other members of this focal-adhesion-associated adaptor-protein family, it has four leucine-rich LD-motifs in the N-terminus and four LIM domains in the C-terminus. It may function in cell type-specific signaling by associating with PYK2, a member of focal adhesion kinase family. As a substrate for a tyrosine kinase in lymphoid cells, this protein may also function in, and be regulated by, tyrosine kinase activity.

Entrez Gene ID

9404

UniProt ID

O60711

Alternative Names

4933402K05Rik; A530083L21Rik; AV278559; LDPL

Function

Transcriptional coactivator for androgen receptor (AR) and serum response factor (SRF). Contributes to the regulation of cell adhesion, spreading and cell migration and acts as a negative regulator in integrin-mediated cell adhesion events. Suppresses the integrin-induced tyrosine phosphorylation of paxillin (PXN). May play a critical role as an adapter protein in the formation of the adhesion zone in osteoclasts. Negatively regulates B-cell antigen receptor (BCR) signaling.

Biological Process

Cell adhesion1 PublicationNAS:ProtInc
Endothelial cell migrationManual Assertion Based On ExperimentIBA:GO_Central
Negative regulation of B cell receptor signaling pathwayManual Assertion Based On ExperimentIDA:UniProtKB
Negative regulation of cell adhesionManual Assertion Based On ExperimentIDA:UniProtKB
Protein-containing complex assemblyManual Assertion Based On ExperimentTAS:ProtInc
Regulation of cell adhesion mediated by integrinIEA:Ensembl
Signal transductionManual Assertion Based On ExperimentTAS:ProtInc
Substrate adhesion-dependent cell spreadingManual Assertion Based On ExperimentIBA:GO_Central
Transforming growth factor beta receptor signaling pathwayManual Assertion Based On ExperimentIBA:GO_Central

Cellular Location

Cytoplasm
Cell junction, focal adhesion
Nucleus
Cytoplasm, perinuclear region
Cell projection, podosome
Cell membrane
Shuttles between the cytoplasm and nucleus. Recruited to the cell membrane following B-cell antigen receptor (BCR) cross-linking in B-cells. Enhanced focal adhesion kinase activity (PTK2/FAK) attenuates its nuclear accumulation and limits its ability to enhance serum response factor (SRF)-dependent gene transcription. Targeting to focal adhesions is essential for its tyrosine phosphorylation in response to bombesin.

PTM

Phosphorylated on tyrosine residues. Phosphorylation on Tyr-72 is important for its inhibitory function. Bombesin stimulates phosphorylation on Tyr-22, Tyr-62 and Tyr-72.

References

Teng, F., Chen, Y., Liao, F., Wang, Y., Jiang, Y. Q., & Wang, Z. Q. (2023). Leupaxin: A Prospective Therapeutic Target for Esophageal Squamous Carcinoma Treatment.

Teng, F., Chen, Y., Liao, F., Wang, Y., Jiang, Y., & Wang, Z. (2023). Elucidating the Correlation between Leupaxin as a Prognostic Biomarker and Immunotherapeutic Efficacy in Esophageal Squamous Cell Carcinoma.

Khan, A., Li, W., Ambreen, A., Wei, D. Q., Wang, Y., & Mao, Y. (2022). A protein coupling and molecular simulation analysis of the clinical mutants of androgen receptor revealed a higher binding for Leupaxin, to increase the prostate cancer invasion and motility. Computers in Biology and Medicine, 146, 105537.

Bonaud, A., Clare, S., Bisio, V., Sowerby, J. M., Yao, S., Ostergaard, H., ... & Espéli, M. (2020). Leupaxin expression is dispensable for B cell immune responses. Frontiers in Immunology, 11, 466.

Klapproth, S., Bromberger, T., Türk, C., Krüger, M., & Moser, M. (2019). A kindlin-3–leupaxin–paxillin signaling pathway regulates podosome stability. Journal of Cell Biology, 218(10), 3436-3454.

Zhu, G. H., Dai, H. P., Shen, Q., & Zhang, Q. (2019). Downregulation of LPXN expression by siRNA decreases the malignant proliferation and transmembrane invasion of SHI‑1 cells. Oncology Letters, 17(1), 135-140.

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Protocols

Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Associated Products

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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