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Mouse Anti-LYAR Recombinant Antibody (CBYJL-2224) (CBMAB-L2472-YJ)

Provided herein is a Mouse monoclonal antibody, which binds to Ly1 Antibody Reactive (LYAR). The antibody can be used for immunoassay techniques, such as WB, ICC.
See all LYAR antibodies

Summary

Host Animal
Mouse
Specificity
Human
Clone
CBYJL-2224
Antibody Isotype
IgG2b
Application
WB, ICC

Basic Information

Immunogen
E. coli-derived recombinant human LYAR, Lys288-Lys379, Accession # Q9NX58.
Specificity
Human
Antibody Isotype
IgG2b
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Buffer
PBS, Trehalose
Storage
Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
Ly1 antibody reactive
Introduction
Diseases associated with LYAR include Neuroblastoma.
Entrez Gene ID
55646
UniProt ID
Q9NX58
Alternative Names
ZLYAR; ZC2HC2; cell growth-regulating nucleolar protein; Ly1 antibody reactive homolog
Function
Plays a role in the maintenance of the appropriate processing of 47S/45S pre-rRNA to 32S/30S pre-rRNAs and their subsequent processing to produce 18S and 28S rRNAs (PubMed:24495227).
Also acts at the level of transcription regulation. Along with PRMT5, binds the gamma-globin (HBG1/HBG2) promoter and represses its expression (PubMed:25092918).
In neuroblastoma cells, may also repress the expression of oxidative stress genes, including CHAC1, HMOX1, SLC7A11, ULBP1 and SNORD41 that encodes a small nucleolar RNA (PubMed:28686580).
Preferentially binds to a DNA motif containing 5'-GGTTAT-3' (PubMed:25092918).
Negatively regulates the antiviral innate immune response by targeting IRF3 and impairing its DNA-binding activity (PubMed:31413131).
In addition, inhibits NF-kappa-B-mediated expression of pro-inflammatory cytokines (PubMed:31413131).
Stimulates phagocytosis of photoreceptor outer segments by retinal pigment epithelial cells (By similarity).
Prevents nucleolin/NCL self-cleavage, maintaining a normal steady-state level of NCL protein in undifferentiated embryonic stem cells (ESCs), which in turn is essential for ESC self-renewal (By similarity).
Biological Process
Erythrocyte developmentManual Assertion Based On ExperimentIMP:UniProtKB
Innate immune responseIEA:UniProtKB-KW
Negative regulation of innate immune responseManual Assertion Based On ExperimentIDA:ARUK-UCL
Negative regulation of transcription by RNA polymerase IIManual Assertion Based On ExperimentIDA:ARUK-UCL
Positive regulation of phagocytosisISS:UniProtKB
Positive regulation of transcription by RNA polymerase IManual Assertion Based On ExperimentIDA:ARUK-UCL
rRNA processingManual Assertion Based On ExperimentIMP:UniProtKB
Cellular Location
Nucleus
Nucleus, nucleolus
Cytoplasm
Cell projection, cilium, photoreceptor outer segment
Component of pre-ribosomal particles, including pre-40S, pre-60S and pre-90S (PubMed:24495227).
Associated with cytoplasmic ribosomes, but not polysomes, as a component of the 60S subunit (PubMed:24990247).
In the retina, predominantly expressed in photoreceptor outer segments (By similarity).
In the nucleolus, colocalizes with nucleolin/NCL, therefore may reside in the dense fibrillar component (DFC) (By similarity).

Zuccato, C., Cosenza, L. C., Zurlo, M., Breveglieri, G., Bianchi, N., Lampronti, I., ... & Gambari, R. (2023). The rs368698783 (G> A) Polymorphism Affecting LYAR Binding to the Aγ-Globin Gene Is Associated with High Fetal Hemoglobin (HbF) in β-Thalassemia Erythroid Precursor Cells Treated with HbF Inducers. International Journal of Molecular Sciences, 24(1), 776.

Chen, L., Jin, C., Liu, H., Feng, R., Li, Z., & Zhang, J. (2021). Analysis of the role of Ly-1 antibody reactive in different cancer types. Bioengineered, 12(2), 9452-9462.

Lu, X. N., Ju, G. J., Wang, Y. X., Wang, Y. L., Wang, K., Chen, J. L., ... & Zang, Q. W. (2021). LYAR promotes the proliferation of non-small cell lung cancer and is associated with poor prognosis. Folia Histochemica et Cytobiologica, 59(4), 282-290.

Sun, X., Wang, G., Ding, P., & Li, S. (2020). LINC00355 promoted the progression of lung squamous cell carcinoma through regulating the miR-466/LYAR axis. Brazilian Journal of Medical and Biological Research, 53.

Izumikawa, K., Ishikawa, H., Yoshikawa, H., Fujiyama, S., Watanabe, A., Aburatani, H., ... & Takahashi, N. (2019). LYAR potentiates rRNA synthesis by recruiting BRD2/4 and the MYST-type acetyltransferase KAT7 to rDNA. Nucleic Acids Research, 47(19), 10357-10372.

Gemmo, C., Breveglieri, G., Marzaro, G., Lampronti, I., Cosenza, L. C., Gasparello, J., ... & Gambari, R. (2019). Surface plasmon resonance based analysis of the binding of LYAR protein to the rs368698783 (G> A) polymorphic Aγ-globin gene sequences mutated in β-thalassemia. Analytical and bioanalytical chemistry, 411, 7699-7707.

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For research use only. Not intended for any clinical use.

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