Mouse Anti-Lypd8 Recombinant Antibody (CBYJL-2574) (CBMAB-L3008-YJ)

Provided herein is a Mouse monoclonal antibody, which binds to Ly6/Plaur Domain Containing 8 (Lypd8). The antibody can be used for immunoassay techniques, such as IHC-Fr, FC, IHC-P.
See all Lypd8 antibodies

Datasheet

Summary

Host Animal

Mouse

Specificity

Mouse

Clone

CBYJL-2574

Antibody Isotype

IgG2b, κ

Application

IHC-Fr, FC, IHC-P

Basic Information

Immunogen

Mouse colon tissue.

Specificity

Mouse

Antibody Isotype

IgG2b, κ

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Buffer

PBS, pH 7.2

Preservative

0.09% Sodium Azide

Storage

Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name

Ly6/Plaur Domain Containing 8

Introduction

Lypd8 encodes a member of the Ly6/PLAUR family of cysteine-rich proteins that plays an important role in the protection of colonic epithelium from flagellated microbiota. LY6/PLAUR domain containing 8 undergoes proteolytic processing to generate a mature, glycosylphosphatidylinositol-anchored protein that is localized to the apical surface of the colonic epithelial cells. Mice lacking LY6/PLAUR domain containing 8 are sensitive to chemically induced intestinal inflammation.

Entrez Gene ID

70163

UniProt ID

Q9D7S0

Alternative Names

2210415F13Rik; ly6/PLAUR domain-containing protein 8

Function

Secreted protein specifically required to prevent invasion of Gram-negative bacteria in the inner mucus layer of the colon epithelium, a portion of the large intestine which is free of commensal microbiota. Prevents invasion of flagellated microbiota by binding to the flagellum of bacteria, such as P.mirabilis, thereby inhibiting bacterial motility in the intestinal lumen. Segregation of intestinal bacteria and epithelial cells in the colon is required to preserve intestinal homeostasis.

Biological Process

Defense response to Gram-negative bacteriumISS:UniProtKB

Cellular Location

Cell membrane
Secreted
Secreted into the lumen of the colon following cleavage of the GPI-anchor.

PTM

Highly N-glycosylated. Not O-glycosylated.
GPI-anchored. The GPI-anchor is cleaved, leading to secretion into the colonic lumen.

References

Xu, J., Qian, J., Zhang, W., Chen, E., Zhang, G., Cao, G., ... & Song, Z. (2021). [Corrigendum] LYPD8 regulates the proliferation and migration of colorectal cancer cells through inhibiting the secretion of IL‑6 and TNF‑α. oncology reports, 45(5), 1-4.

Hsu, C. C., Okumura, R., Motooka, D., Sasaki, R., Nakamura, S., Iida, T., & Takeda, K. (2021). Alleviation of colonic inflammation by Lypd8 in a mouse model of inflammatory bowel disease. International Immunology, 33(7), 359-372.

Ara, T., Hashimoto, D., Hayase, E., Noizat, C., Kikuchi, R., Hasegawa, Y., ... & Teshima, T. (2020). Intestinal goblet cells protect against GVHD after allogeneic stem cell transplantation via Lypd8. Science Translational Medicine, 12(550), eaaw0720.

Okumura, R., Kodama, T., Hsu, C. C., Sahlgren, B. H., Hamano, S., Kurakawa, T., ... & Takeda, K. (2020). Lypd8 inhibits attachment of pathogenic bacteria to colonic epithelia. Mucosal Immunology, 13(1), 75-85.

Okada, T., Kanda, T., Ueda, N., Ikebuchi, Y., Hashiguchi, K., Nakao, K., & Isomoto, H. (2020). IL-8 and LYPD8 expression levels are associated with the inflammatory response in the colon of patients with ulcerative colitis. Biomedical reports, 12(4), 193-198.

Xu, J., Qian, J., Zhang, W., Chen, E., Zhang, G., Cao, G., ... & Song, Z. (2019). LYPD8 regulates the proliferation and migration of colorectal cancer cells through inhibiting the secretion of IL‑6 and TNF‑α Corrigendum in/10.3892/or. 2021.8004. Oncology reports, 41(4), 2389-2395.

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Protocols

Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Associated Products

Related Products

Mouse Anti-LYPD8 Recombinant Antibody (CBYJL-2247)

Mouse Anti-Lypd8 Recombinant Antibody (CBYJL-2575)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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