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Mouse Anti-PLA2G15 Recombinant Antibody (3B11) (CBMAB-A5142-LY)

The product is antibody recognizes LYPLA3. The antibody 3B11 immunoassay techniques such as: WB, ELISA.
See all PLA2G15 antibodies

Summary

Host Animal
Mouse
Specificity
Human, Mouse, Rat
Clone
3B11
Antibody Isotype
IgG2a, κ
Application
WB, ELISA

Basic Information

Immunogen
LYPLA3 (NP_036452, 314 a.a. ~ 412 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Specificity
Human, Mouse, Rat
Antibody Isotype
IgG2a, κ
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Purity
> 95% Purity determined by SDS-PAGE.
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name
Phospholipase A2 group XV
Introduction
Lysophospholipases are enzymes that act on biological membranes to regulate the multifunctional lysophospholipids. The protein encoded by this gene hydrolyzes lysophosphatidylcholine to glycerophosphorylcholine and a free fatty acid. This enzyme is present in the plasma and thought to be associated with high-density lipoprotein. A later paper contradicts the function of this gene. It demonstrates that this gene encodes a lysosomal enzyme instead of a lysophospholipase and has both calcium-independent phospholipase A2 and transacylase activities. [provided by RefSeq]
Entrez Gene ID
Human23659
Mouse192654
Rat361401
UniProt ID
HumanQ8NCC3
MouseQ8VEB4
RatQ675A5
Alternative Names
ACS; DKFZp564A0122; GXVPLA2; LLPL; LPLA2; LYPLA3
Function
Has dual calcium-independent phospholipase and O-acyltransferase activities with a potential role in glycerophospholipid homeostasis and remodeling of acyl groups of lipophilic alcohols present in acidic cellular compartments (PubMed:10092508, PubMed:11790796, PubMed:20410020, PubMed:23958596, PubMed:29724779, PubMed:25727495).
Catalyzes hydrolysis of the ester bond of the fatty acyl group attached at sn-1 or sn-2 position of phospholipids (phospholipase A1 or A2 activity) and transfer it to the hydroxyl group at the first carbon of lipophilic alcohols (O-acyltransferase activity) (PubMed:10092508, PubMed:11790796, PubMed:20410020, PubMed:23958596, PubMed:29724779, PubMed:25727495).
Among preferred fatty acyl donors are phosphatidylcholines, phosphatidylethanolamines, phosphatidylglycerols and phosphatidylserines (PubMed:29724779).
Favors sn-2 over sn-1 deacylation of unsaturated fatty acyl groups of phosphatidylcholines and phosphatidylethanolamines (By similarity).
Among preferred fatty acyl acceptors are natural lipophilic alcohols including short-chain ceramide N-acetyl-sphingosine (C2 ceramide), alkylacylglycerols, monoacylglycerols, and acylethanolamides such as anandamide and oleoylethanolamide (PubMed:29724779).
Selectively hydrolyzes the sn-1 fatty acyl group of truncated oxidized phospholipids and may play a role in detoxification of reactive oxidized phospholipids during oxidative stress (PubMed:30830753).
Required for normal phospholipid degradation in alveolar macrophages with potential implications in pulmonary surfactant clearance (By similarity).
At neutral pH, hydrolyzes the sn-1 fatty acyl group of the lysophosphatidylcholines (PubMed:10092508).
Biological Process
Ceramide metabolic processManual Assertion Based On ExperimentIDA:UniProtKB
Diacylglycerol biosynthetic processISS:UniProtKB
Fatty acid catabolic processManual Assertion Based On ExperimentTAS:ProtInc
Glycerophospholipid metabolic processManual Assertion Based On ExperimentIDA:UniProtKB
Lipid metabolic processManual Assertion Based On ExperimentIBA:GO_Central
Phosphatidylcholine catabolic processISS:UniProtKB
Phosphatidylcholine metabolic processManual Assertion Based On ExperimentIDA:UniProtKB
Phosphatidylethanolamine catabolic processISS:UniProtKB
Phosphatidylglycerol metabolic processManual Assertion Based On ExperimentIDA:UniProtKB
Phosphatidylserine metabolic processManual Assertion Based On ExperimentIDA:UniProtKB
Phospholipid metabolic processTAS:Reactome
Cellular Location
Lysosome
Secreted
Membrane
PTM
N-glycosylated (PubMed:11790796, PubMed:23958596).
N-glycosylation is important for maturation of the enzyme and normal subcellular location (PubMed:23958596).
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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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