Mouse Anti-LYVE1 Recombinant Antibody (4G1) (CBMAB-L2523-YJ)

Name: Mouse Anti-LYVE1 Recombinant Antibody (4G1)
SKU: CBMAB-L2523-YJ
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

4G1

Application

WB, FC, ELISA

Immunogen

Recombinant human LYVE-1 (25-235 aa) purified from E. coli.

Specificity

Human

Antibody Isotype

IgG2b

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Buffer

PBS, pH 7.4

Storage

Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Epitope

aa 25-235

More Infomation

Target

Full Name

Lymphatic Vessel Endothelial Hyaluronan Receptor 1

Introduction

LYVE1 is a type I integral membrane glycoprotein. LYVE1 acts as a receptor and binds to both soluble and immobilized hyaluronan. This protein may play a role in lymphatic hyaluronan transport and have a role in tumor metastasis.

Entrez Gene ID

10894

UniProt ID

Q9Y5Y7

Alternative Names

HAR; XLKD1; LYVE-1; CRSBP-1; lymphatic vessel endothelial hyaluronic acid receptor 1; cell surface retention sequence binding protein-1; extracellular link domain-containing 1; extracellular link domain-containing protein 1; hyaluronic acid receptor

Function

Ligand-specific transporter trafficking between intracellular organelles (TGN) and the plasma membrane. Plays a role in autocrine regulation of cell growth mediated by growth regulators containing cell surface retention sequence binding (CRS). May act as a hyaluronan (HA) transporter, either mediating its uptake for catabolism within lymphatic endothelial cells themselves, or its transport into the lumen of afferent lymphatic vessels for subsequent re-uptake and degradation in lymph nodes (PubMed:10037799).
Binds to pericelluar hyaluronan matrices deposited on the surface of leukocytes and facilitates cell adhesion and migration through lymphatic endothelium (PubMed:26823460).

Biological Process

Anatomical structure morphogenesisManual Assertion Based On ExperimentTAS:ProtInc
Cell-matrix adhesionManual Assertion Based On ExperimentTAS:ProtInc
Glycosaminoglycan catabolic processIEA:Ensembl
Positive regulation of cellular extravasationManual Assertion Based On ExperimentIDA:UniProtKB
Response to woundingManual Assertion Based On ExperimentTAS:ProtInc

Cellular Location

Cell membrane
Localized to the plasma membrane and in vesicles near extranuclear membranes which may represent trans-Golgi network (TGN) and endosomes/prelysosomeal compartments. Undergoes ligand-dependent internalization and recycling at the cell surface. Localizes at cell-cell junctions.

Topology

Extracellular: 20-238
Helical: 239-259
Cytoplasmic: 260-322

PTM

O-glycosylated.

Anstee, J. E., Feehan, K. T., Opzoomer, J. W., Dean, I., Muller, H. P., Bahri, M., ... & Arnold, J. N. (2023). LYVE-1+ macrophages form a collaborative CCR5-dependent perivascular niche that influences chemotherapy responses in murine breast cancer. Developmental Cell, 58(17), 1548-1561.

Burger, F., Baptista, D., Roth, A., Brandt, K. J., da Silva, R. F., Montecucco, F., ... & Miteva, K. (2022). Single-cell RNA-seq reveals a crosstalk between hyaluronan receptor LYVE-1-expressing macrophages and vascular smooth muscle cells. Cells, 11(3), 411.

Kieu, T. Q., Tazawa, K., Kawashima, N., Noda, S., Fujii, M., Nara, K., ... & Okiji, T. (2022). Kinetics of LYVE-1-positive M2-like macrophages in developing and repairing dental pulp in vivo and their pro-angiogenic activity in vitro. Scientific reports, 12(1), 5176.

Jauch, A. S., Wohlfeil, S. A., Weller, C., Dietsch, B., Häfele, V., Stojanovic, A., ... & Géraud, C. (2022). Lyve-1 deficiency enhances the hepatic immune microenvironment entailing altered susceptibility to melanoma liver metastasis. Cancer Cell International, 22(1), 1-17.

Karam, M., Janbon, H., Malkinson, G., & Brunet, I. (2022). Heterogeneity and developmental dynamics of LYVE-1 perivascular macrophages distribution in the mouse brain. Journal of Cerebral Blood Flow & Metabolism, 42(10), 1797-1812.

Sethy, C., Goutam, K., Das, B., Dash, S. R., & Kundu, C. N. (2021). Nectin-4 promotes lymphangiogenesis and lymphatic metastasis in breast cancer by regulating CXCR4-LYVE-1 axis. Vascular Pharmacology, 140, 106865.

Ulndreaj, A., Li, A., Chen, Y., Besla, R., Pacheco, S., Althagafi, M. G., ... & Byrne, J. S. (2021). Adventitial recruitment of Lyve-1− macrophages drives aortic aneurysm in an angiotensin-2-based murine model. Clinical Science, 135(10), 1295-1309.

Brezovakova, V., & Jadhav, S. (2020). Identification of Lyve‐1 positive macrophages as resident cells in meninges of rats. Journal of Comparative Neurology, 528(12), 2021-2032.

Cho, H., Shen, G. Q., Wang, X., Wang, F., Archacki, S., Li, Y., ... & Wang, Q. K. (2019). Long noncoding RNA ANRIL regulates endothelial cell activities associated with coronary artery disease by up-regulating CLIP1, EZR, and LYVE1 genes. Journal of biological chemistry, 294(11), 3881-3898.

Jackson, D. G. (2019). Hyaluronan in the lymphatics: the key role of the hyaluronan receptor LYVE-1 in leucocyte trafficking. Matrix Biology, 78, 219-235.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

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