Mouse Anti-MAEA (AA 12-66) Recombinant Antibody (CBFYE-1499) (CBMAB-E2132-FY)

This product is mouse antibody that recognizes MAEA. The antibody CBFYE-1499 can be used for immunoassay techniques such as: WB, ICC.
See all MAEA antibodies

Datasheet

Summary

Host Animal

Mouse

Specificity

Human, Mouse, Rat

Clone

CBFYE-1499

Antibody Isotype

IgG1

Application

WB, ICC

Basic Information

Immunogen

E.coli-derived recombinant human EMP/MAEA, Met12-Ser66

Specificity

Human, Mouse, Rat

Antibody Isotype

IgG1

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Lyophilized

Buffer

PBS, trehalose

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Epitope

AA 12-66

Target

Full Name

MACROPHAGE ERYTHROBLAST ATTACHER

Introduction

This gene encodes a protein that mediates the attachment of erythroblasts to macrophages. This attachment promotes terminal maturation and enucleation of erythroblasts, presumably by suppressing apoptosis. The encoded protein is an integral membrane protein with the N-terminus on the extracellular side and the C-terminus on the cytoplasmic side of the cell. Alternative splicing results in multiple transcript variants.

Entrez Gene ID

Human	10296
Mouse	59003
Rat	298982

UniProt ID

Human	Q7L5Y9
Mouse	Q4VC33
Rat	Q5RKJ1

Alternative Names

Macrophage Erythroblast Attacher; Cell Proliferation-Inducing Gene 5 Protein; Human Lung Cancer Oncogene 10 Protein; Erythroblast Macrophage Protein; HLC-10; EMP; GID Complex Subunit 9, FYV10 Homolog (S. Cerevisiae)

Function

Core component of the CTLH E3 ubiquitin-protein ligase complex that selectively accepts ubiquitin from UBE2H and mediates ubiquitination and subsequent proteasomal degradation of the transcription factor HBP1. MAEA and RMND5A are both required for catalytic activity of the CTLH E3 ubiquitin-protein ligase complex. MAEA is required for normal cell proliferation. The CTLH E3 ubiquitin-protein ligase complex is not required for the degradation of enzymes involved in gluconeogenesis, such as FBP1 (By similarity).
Plays a role in erythroblast enucleation during erythrocyte maturation and in the development of mature macrophages (By similarity).
Mediates the attachment of erythroid cell to mature macrophages; this MAEA-mediated contact inhibits erythroid cell apoptosis (By similarity).
Participates in erythroblastic island formation, which is the functional unit of definitive erythropoiesis. Associates with F-actin to regulate actin distribution in erythroblasts and macrophages (By similarity).
May contribute to nuclear architecture and cells division events (By similarity).

Biological Process

Cell adhesionIEA:Ensembl
Cell cycleIEA:UniProtKB-KW
Cell divisionIEA:UniProtKB-KW
Cytoskeleton organizationIEA:Ensembl
Enucleate erythrocyte developmentIEA:Ensembl
Erythrocyte maturationIEA:UniProtKB-KW
Negative regulation of myeloid cell apoptotic processIEA:Ensembl
Proteasome-mediated ubiquitin-dependent protein catabolic processManual Assertion Based On ExperimentIBA:GO_Central

Cellular Location

Cytoplasm
Nucleus, nucleoplasm
Nucleus matrix
Cell membrane
Cytoplasm, cytoskeleton
Detected in a nuclear, speckled-like pattern (By similarity).
Localized with condensed chromatin at prophase; Detected in nuclear spindle poles at metaphase and in the contractile ring during telophase and cytokinesis (By similarity).
Present in cytoplasm, nuclear matrix and at the cell surface in macrophages; predominantly nuclear in immature macrophages and predominantly detected at the cell surface in mature macrophages. Colocalizes with F-actin in macrophages (By similarity).

PTM

Autoubiquitinated as component of the CTLH E3 ubiquitin-protein ligase complex (in vitro).

References

Zhan, Q., Wang, J., Zhang, H., & Zhang, L. (2023). E3 ubiquitin ligase on the biological properties of hematopoietic stem cell. Journal of Molecular Medicine, 1-14.

Lu, T., Li, Y., Pan, M., Yu, D., Wang, Z., Liu, C., & Hu, G. (2022). TBC1D14 inhibits autophagy to suppress lymph node metastasis in head and neck squamous cell carcinoma by downregulating macrophage erythroblast attacher. International Journal of Biological Sciences, 18(5), 1795.

Wei, Q., Pinho, S., Dong, S., Pierce, H., Li, H., Nakahara, F., ... & Frenette, P. S. (2021). MAEA is an E3 ubiquitin ligase promoting autophagy and maintenance of haematopoietic stem cells. Nature communications, 12(1), 2522.

Maitland, M. E., Onea, G., Chiasson, C. A., Wang, X. U., Ma, J., Moor, S. E., ... & Schild-Poulter, C. (2019). The mammalian CTLH complex is an E3 ubiquitin ligase that targets its subunit muskelin for degradation. Scientific reports, 9(1), 9864.

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Protocols

Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Associated Products

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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