MAEA Antibodies

Background

The MAEA gene encodes a protein called the Macrophage Erythroblast Attacher, which is mainly expressed in the cell nucleus and participates in transcriptional regulation and the process of cell mitosis as an RNA-binding protein. It maintains genomic stability by regulating the spindle assembly checkpoint mechanism to ensure the accurate separation of chromosomes. Mutations in this gene are associated with human developmental abnormalities and bone marrow failure syndrome, especially playing a key role in certain hereditary blood diseases. Since its first identification in 1997, the structural and functional research of MAEA has deepened people's understanding of the mechanisms of cell cycle regulation and hematopoietic development. Its complex molecular interaction network has become an important model for cancer and genetic disease research.

Structure Function Application Advantage Our Products

Structure of MAEA

MAEA is a nucleoprotein with a molecular weight of approximately 65 kDa, and its precise molecular weight varies slightly among different species.

Species	Human	Mouse	Zebrafish
Molecular Weight (kDa)	65.2	64.8	63.5
Primary Structural Differences	Contains nuclear localization signal and RNA binding domain	Amino acid substitutions are present in the C-terminal domain	Lack of mammalian specific regulatory sequences

This protein is composed of 543 amino acids and forms a functional dimer through its coiled helical domain. The secondary structure of MAEA contains typical α -helices and β -folds, among which the conserved RNA recognition motif (RRM) constitutes the key nucleic acid binding interface. The nuclear localization signal at the N-terminal of the protein ensures its intracnuclear localization, while the oligomerization domain at the C-terminal mediates the assembly of the protein complex. This multi-level structural feature enables it to effectively regulate gene expression and maintain genomic stability.

Fig. 1:Protein-protein interactions network for MAEA and its related proteins. Fig. 1 Protein-protein interactions network for MAEA and its related proteins.¹

Key structural properties of MAEA:

Nuclear protein complexes composed of multiple domains
Conserved RNA recognition modalities and nuclear localization signals
Coiled helical dimerization interface

Functions of MAEA

The main function of the MAEA gene is to maintain genomic stability and regulate the cell cycle process. At the same time, it also participates in key cellular activities such as transcriptional regulation and RNA metabolism.

Function	Description
Mitotic regulation	Ensure accurate chromosome separation through spindle assembly checkpoints to prevent aneuploidy formation.
Transcriptional regulation	As a component of the nucleoprotein complex, it regulates the expression of genes related to cell differentiation.
Red blood cell maturation	In the process of hematopoiesis, it mediates the interaction between macrophages and erythroblasts and promotes the maturation of red blood cells.
DNA damage response	Participate in the stress response of cells to DNA damage and maintain genomic integrity.
RNA metabolism	After RNA binding ability to participate in transcription regulation, and the influence of gene expression.

MAEA coordinates multiple cellular processes by forming a dynamic protein complex network, and its dysfunction is closely related to bone marrow failure, developmental abnormalities and tumorigenesis.

Applications of MAEA and MAEA Antibody in Literature

1. Wei, Qiaozhi, et al. "MAEA is an E3 ubiquitin ligase promoting autophagy and maintenance of haematopoietic stem cells." Nature Communications 12.1 (2021): 2522. https://doi.org/10.1038/s41467-021-22749-1

This article finds that membrane-associated E3 ubiquitin ligase MAEA is crucial for maintaining the homeostasis of hematopoietic stem cells. The absence of MAEA can lead to resting impairment of stem cells, abnormal proliferation and lymphatic differentiation defects by stabilizing the expression of cytokine receptors (such as MPL and FLT3) and hindering autophagic flow. However, activating autophagy or inhibiting receptor kinases can reverse this phenotype.

2. Feng, Yanchun, et al. "The E3 ubiquitin ligase MAEA promotes macrophage phagocytosis and inhibits gastrointestinal cancer progression by mediating PARP1 ubiquitination and degradation." International Journal of Biological Sciences 21.4 (2025): 1784. https://doi.org/10.7150/ijbs.102796

Research has found that the E3 ubiquitin ligase MAEA inhibits tumor proliferation and chemotherapy resistance in gastrointestinal cancers by mediating the ubiquitination and degradation of PARP1. Meanwhile, MAEA can inhibit the polarization of M2-type macrophages and enhance their phagocytic activity, thereby curbing tumor development. Low expression of MAEA indicates a poor prognosis for patients, and its mechanism provides a new target for the treatment of GIC.

3. Cai, Xuan, et al. "Common variants in MAEA gene contributed the susceptibility to osteoporosis in Han Chinese postmenopausal women." Journal of orthopaedic surgery and research 16.1 (2021): 38. https://doi.org/10.1186/s13018-020-02140-4

This study found in the Han Chinese population that the rs6815464 polymorphism of the MAEA gene was significantly associated with the risk of postmenopausal osteoporosis. Its C allele can reduce the risk of the disease and is associated with a decrease in MAEA protein levels in the blood, suggesting that MAEA plays an important role in the onset of osteoporosis.

4. Piloto, Ana Margarida, et al. "Plastic antibodies tailored on quantum dots for an optical detection of myoglobin down to the femtomolar range." Scientific reports 8.1 (2018): 4944. https://doi.org/10.3389/fonc.2025.1597750

Research has found that in osteosarcoma patients with a low chemotherapy-induced necrosis rate, the expression of E3 ubiquitin ligase MAEA is significantly upregulation and has been identified as a core hub protein. This indicates that MAEA may play an important role in the chemotherapy resistance process of osteosarcoma, providing a new potential therapeutic target for improving the therapeutic effect of such patients.

5. Jiang, Ke, et al. "Optimization of hydrogen production in Enterobacter aerogenes by Complex I peripheral fragments destruction and maeA overexpression." Microbial Cell Factories 22.1 (2023): 137. https://doi.org/10.1186/s12934-023-02155-6

This study collaboratively modified the metabolic pathways of Escherichia coli by knocking out the nuoEFG gene and overexpressing the maeA gene. This strategy significantly enhanced the hydrogen production capacity of the strain, with the formic acid pathway flux increasing by 257% and the NADH pathway flux rising by 13%. Ultimately, the total hydrogen output was raised by 18%, providing a new idea for multi-gene combination modification in microbial hydrogen production.

Creative Biolabs: MAEA Antibodies for Research

Creative Biolabs specializes in the production of high-quality MAEA antibodies for research and industrial applications. Our portfolio includes monoclonal antibodies tailored for ELISA, Flow Cytometry, Western blot, immunohistochemistry, and other diagnostic methodologies.

Custom MAEA Antibody Development: Tailor-made solutions to meet specific research requirements.
Bulk Production: Large-scale antibody manufacturing for industry partners.
Technical Support: Expert consultation for protocol optimization and troubleshooting.
Aliquoting Services: Conveniently sized aliquots for long-term storage and consistent experimental outcomes.

For more details on our MAEA antibodies, custom preparations, or technical support, contact us at email.

Reference

Cai, Xuan, et al. "Common variants in MAEA gene contributed the susceptibility to osteoporosis in Han Chinese postmenopausal women." Journal of orthopaedic surgery and research 16.1 (2021): 38. https://doi.org/10.1186/s13018-020-02140-4