Rabbit Anti-MAGI3 Recombinant Antibody (CBFYM-1340) (CBMAB-M1499-FY)

This product is rabbit antibody that recognizes MAGI3. The antibody CBFYM-1340 can be used for immunoassay techniques such as: IF, IP, WB.
See all MAGI3 antibodies

Datasheet

Summary

Host Animal

Rabbit

Specificity

Human

Clone

CBFYM-1340

Application

IF, IP, WB

Basic Information

Immunogen

Synthetic peptide corresponding to residues surrounding the fusion site of MAGI3-Akt3 Fusion protein

Specificity

Human

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

HEPES, pH 7.5, 0.15 M NaCl, 0.01% BSA, 50% glycerol

Preservative

0.02% Sodium azide

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name

MEMBRANE ASSOCIATED GUANYLATE KINASE, WW AND PDZ DOMAIN CONTAINING 3

Introduction

MAGI3 is a Protein Coding gene. Diseases associated with MAGI3 include Lymphocytic Colitis. Among its related pathways are Sertoli-Sertoli Cell Junction Dynamics and Ras signaling pathway. Gene Ontology annotations related to this gene include frizzled binding and guanylate kinase activity. An important paralog of this gene is MAGI2.

Entrez Gene ID

260425

UniProt ID

Q5TCQ9

Alternative Names

Membrane Associated Guanylate Kinase, WW And PDZ Domain Containing 3; Membrane-Associated Guanylate Kinase Inverted 3; MAGI-3; Membrane-Associated Guanylate Kinase, WW And PDZ Domain-Containing Protein 3; Membrane-Associated Guanylate Kinase-Related 3; DJ730K3.2; KIAA1634

Function

Acts as a scaffolding protein at cell-cell junctions, thereby regulating various cellular and signaling processes. Cooperates with PTEN to modulate the kinase activity of AKT1. Its interaction with PTPRB and tyrosine phosphorylated proteins suggests that it may link receptor tyrosine phosphatase with its substrates at the plasma membrane. In polarized epithelial cells, involved in efficient trafficking of TGFA to the cell surface. Regulates the ability of LPAR2 to activate ERK and RhoA pathways. Regulates the JNK signaling cascade via its interaction with FZD4 and VANGL2.

Biological Process

Apoptotic process1 PublicationNAS:UniProtKB
Intracellular signal transduction1 PublicationNAS:UniProtKB
Regulation of JNK cascadeIEA:InterPro
Signal transductionManual Assertion Based On ExperimentIBA:GO_Central

Cellular Location

Cell membrane
Cell junction, tight junction
Nucleus
Concentrates in specific sites at the plasma membrane and in the nucleus. In epithelial cells, it localizes at tight junctions (By similarity).

PTM

Ubiquitinated following interaction with HPV E6 protein, leading to its degradation by the proteasome. Degradation is independent of E6AP ubiquitin ligase complex.

References

HE, J., Gu, S., Liu, H., Zhang, L., Lu, W., Zhao, C., ... & Feng, D. (2022). Low MAGI3 expression promotes Wnt/β-catenin signaling to mediate metastasis and rapalogs resistance of clear cell renal cell carcinoma.

Wang, H., Yang, W., Qin, Q., Yang, X., Yang, Y., Liu, H., ... & He, J. (2022). E3 ubiquitin ligase MAGI3 degrades c-Myc and acts as a predictor for chemotherapy response in colorectal cancer. Molecular Cancer, 21(1), 1-19.

Xu, M., & Yu, T. (2022). MiR-20b-5p contributes to the dysfunction of vascular smooth muscle cells by targeting MAGI3 in hypertension. Journal of Molecular Histology, 53(2), 187-197.

Yang, Z., Liu, H., Song, R., Lu, W., Wang, H., Gu, S., ... & He, J. (2021). Reduced MAGI3 level by HPV18E6 contributes to Wnt/β‐catenin signaling activation and cervical cancer progression. FEBS Open bio, 11(11), 3051-3062.

Zhang, Q., Ye, F., Guo, S., Xiao, W., Zhang, J., Qu, Y., & Zhang, J. (2021). Knockdown of lncRNA RMST protect against myocardial infarction through regulating miR-5692 and MAGI3 axis. American Journal of Translational Research, 13(4), 3906.

Weng, Q., Chen, M., Yang, W., Li, J., Fan, K., Xu, M., ... & Ji, J. (2020). Integrated analyses identify miR‐34c‐3p/MAGI3 axis for the Warburg metabolism in hepatocellular carcinoma. The FASEB Journal, 34(4), 5420-5434.

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Protocols

Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Associated Products

Related Products

Mouse Anti-MAGI3 Recombinant Antibody (CBFYM-1341)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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