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Mouse Anti-MAK Recombinant Antibody (4E9) (CBMAB-A5179-LY)

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Datasheet
SDS
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Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal
Mouse
Clone
4E9
Application
WB, ELISA
Immunogen
MAK (AAH39825, 358 a.a. ~ 457 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Specificity
Human
Antibody Isotype
IgG2a, κ
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Purity
> 95% Purity determined by SDS-PAGE.
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.
More Infomation

Target

Full Name
male germ cell-associated kinase
Introduction
The product of this gene is a serine/threonine protein kinase related to kinases involved in cell cycle regulation. It is expressed almost exclusively in the testis, primarily in germ cells. Studies of the mouse and rat homologs have localized the kinase to the chromosomes during meiosis in spermatogenesis, specifically to the synaptonemal complex that exists while homologous chromosomes are paired. There is, however, a study of the mouse homolog that has identified high levels of expression in developing sensory epithelia so its function may be more generalized. [provided by RefSeq]
Entrez Gene ID
4117
UniProt ID
P20794
Alternative Names
dJ417M14.2
Function
Essential for the regulation of ciliary length and required for the long-term survival of photoreceptors (By similarity).
Phosphorylates FZR1 in a cell cycle-dependent manner. Plays a role in the transcriptional coactivation of AR. Could play an important function in spermatogenesis. May play a role in chromosomal stability in prostate cancer cells.
Biological Process
Cell differentiationIEA:UniProtKB-KW
Cilium assemblyManual Assertion Based On ExperimentIBA:GO_Central
Intracellular signal transductionManual Assertion Based On ExperimentIBA:GO_Central
Intraciliary transportManual Assertion Based On ExperimentIBA:GO_Central
Negative regulation of non-motile cilium assemblyIEA:Ensembl
Photoreceptor cell maintenanceISS:UniProtKB
Protein autophosphorylationManual Assertion Based On ExperimentIMP:UniProtKB
Protein phosphorylationManual Assertion Based On ExperimentIDA:UniProtKB
Spermatogenesis1 PublicationNAS:UniProtKB
Cellular Location
Nucleus
Cytoplasm, cytoskeleton, microtubule organizing center, centrosome
Cytoplasm, cytoskeleton, spindle
Midbody
Cell projection, cilium, photoreceptor outer segment
Photoreceptor inner segment
Localized in both the connecting cilia and the outer segment axonemes (By similarity).
Localized uniformly in nuclei during interphase, to the mitotic spindle and centrosomes during metaphase and anaphase, and also to midbody at anaphase until telophase.
Involvement in disease
Retinitis pigmentosa 62 (RP62):
A retinal dystrophy belonging to the group of pigmentary retinopathies. Retinitis pigmentosa is characterized by retinal pigment deposits visible on fundus examination and primary loss of rod photoreceptor cells followed by secondary loss of cone photoreceptors. Patients typically have night vision blindness and loss of midperipheral visual field. As their condition progresses, they lose their far peripheral visual field and eventually central vision as well.
PTM
Autophosphorylated. Phosphorylated on serine and threonine residues.

Matsushime, H., Jinno, A., Takagi, N., & Shibuya, M. (2023). A novel mammalian protein kinase gene (mak) is highly expressed in testicular germ cells at and after meiosis. Molecular and Cellular Biology.

Gibson‐Corley, K. N., Cranston, C., Anfinson, K., Kaalberg, E., Han, I., Slusarski, D. C., ... & Tucker, B. A. (2019). Development of gene augmentation strategy for the treatment of MAK associated Retinitis Pigmentosa. The FASEB Journal, 33(S1), 802-20.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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